Metabolomics of transgenic maize combining Fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extraction

León, Carlos; Rodríguez‐Meizoso, Irene; Lucio, Marianna; García‐Cañas, Virginia; Ibáñez, Elena; Schmitt‐Kopplin, Philippe; Cifuentes, Alejandro

doi:10.1016/j.chroma.2009.04.092

Cited by 93 publications

(54 citation statements)

References 48 publications

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“…Using a different MON810 line, grown in a growth chamber, Piccioni et al (2009) identified 40 water-soluble metabolites and found a higher concentration for five compounds in the GE extracts (all different from those of Manetti et al [2006]). Leon et al (2009) found increases in some metabolites from specific metabolisms (purine, amino acid, arachidonic acid, linoleic acid) in three field-grown MON810 lines compared with their controls. There were only 10 metabolites with increased levels when two different technologies were compared.…”

Section: Maizementioning

confidence: 99%

“…for GE crop assessment but would provide little or no added value for food safety (Chassy, 2010), since it does not yet surpass the currently used analytical methods (Harrigan et al, 2010). In addition, when studies have used different metabolomic technologies simultaneously, discrepancies in the results were obvious (Leon et al, 2009). …”

Section: Metabolomics Versus Traditional Analytical Chemistrymentioning

confidence: 99%

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Evaluation of Genetically Engineered Crops Using Transcriptomic, Proteomic, and Metabolomic Profiling Techniques

2011

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Section: Maizementioning

confidence: 99%

Section: Metabolomics Versus Traditional Analytical Chemistrymentioning

confidence: 99%

Evaluation of Genetically Engineered Crops Using Transcriptomic, Proteomic, and Metabolomic Profiling Techniques

2011

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“…This kind of information is also very important for the determination of metabolites in genetically modifi ed food (138,198). However, with corresponding sample preparations and in--front separation by liquid chromatography or capillary electrophoresis, FT-ICR MS (199), MALDI-TOF MS (200) and other mass spectrometry techniques (201) have been proven to be equally effi cient analytical tools for these kinds of tests.…”

Section: Hybrid Mass Spectrometers -Orbitrap and Other Instrumentsmentioning

confidence: 99%

Foodomics and Food Safety: Where We Are

Andjelković

Gajdošik

Gašo-Sokač

et al. 2017

Food Technol. Biotechnol.

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SummaryThe power of foodomics as a discipline that is now broadly used for quality assurance of food products and adulteration identifi cation, as well as for determining the safety of food, is presented. Concerning sample preparation and application, maintenance of highly sophisticated instruments for both high-performance and high-throughput techniques, and analysis and data interpretation, special att ention has to be paid to the development of skilled analysts. The obtained data shall be integrated under a strong bioinformatics environment. Modern mass spectrometry is an extremely powerful analytical tool since it can provide direct qualitative and quantitative information about a molecule of interest from only a minute amount of sample. Quality of this information is infl uenced by the sample preparation procedure, the type of mass spectrometer used and the analyst's skills. Technical advances are bringing new instruments of increased sensitivity, resolution and speed to the market. Other methods presented here give additional information and can be used as complementary tools to mass spectrometry or for validation of obtained results. Genomics and transcriptomics, as well as affi nity-based methods, still have a broad use in food analysis. Serious drawbacks of some of them, especially the affi nity-based methods, are the cross-reactivity between similar molecules and the infl uence of complex food matrices. However, these techniques can be used for pre-screening in order to reduce the large number of samples. Great progress has been made in the application of bioinformatics in foodomics. These developments enabled processing of large amounts of generated data for both identifi cation and quantifi cation, and for corresponding modeling.

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“…The assignment of peaks to metabolites not typically associated with a genus or family would almost certainly require extensive validation in a Regulatory environment. Leon et al (2009) utilized FT-MS on the same samples assessed by Levandi et al (2008). This allowed coverage of 5500 mass signals of which approximately 1000 could be assigned an elemental composition.…”

Section: Another Challenge In Establishing a Coherent Literature On Tmentioning

confidence: 99%

Challenges for Metabolomics as a Tool in Safety Assessments

Harrigan¹,

Chassy²

2012

Metabolomics

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Metabolomics of transgenic maize combining Fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extraction

Cited by 93 publications

References 48 publications

Evaluation of Genetically Engineered Crops Using Transcriptomic, Proteomic, and Metabolomic Profiling Techniques

Evaluation of Genetically Engineered Crops Using Transcriptomic, Proteomic, and Metabolomic Profiling Techniques

Foodomics and Food Safety: Where We Are

Challenges for Metabolomics as a Tool in Safety Assessments

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