Metabolomics of the human aqueous humor

Snytnikova, Olga A.; Khlichkina, Anastasiya A.; Yanshole, Lyudmila V.; Yanshole, Vadim V.; Iskakov, I. A.; Egorova, E. V.; Stepakov, Denis A.; Новоселов, В. П.; Tsentalovich, Yuri P.

doi:10.1007/s11306-016-1144-0

Cited by 33 publications

(35 citation statements)

References 33 publications

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“…Dried extracts were re-dissolved in 600 µL of D 2 O (99.9%, Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing 6 µM sodium 4,4-dimethyl-4-silapentane-1-sulfonate (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) as an internal standard and 20 mM deuterated phosphate buffer to maintain pH 7.4. All 1 H NMR measurements were carried out as described previously [37] with the use of the AVANCE III HD 700 MHz NMR spectrometer (Bruker BioSpin, Ettlingen, Germany). The baseline processing and integration were done using the MestReNova v12.0 software.…”

Section: Metabolite Extraction and Nmr Spectroscopymentioning

confidence: 99%

“…The baseline processing and integration were done using the MestReNova v12.0 software. The metabolite signal assignment was confirmed by the addition of authentic compounds into samples and using the Human Metabolome Database [38,39] and our own experience in the metabolomic profiling of animal and human tissues and biofluids [37,[40][41][42][43]. The concentrations of metabolites in samples were calculated by the peak area integration respectively to the internal standard.…”

Section: Metabolite Extraction and Nmr Spectroscopymentioning

confidence: 99%

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Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

et al. 2020

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Growing evidence suggests that intestinal mucosa homeostasis impacts immunity, metabolism, the Central Nervous System (CNS), and behavior. Here, we investigated the effect of the monosaccharide fucose on inflammation, metabolism, intestinal microbiota, and social behavior in the Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. Our data show that chronic colitis is accompanied by the decrease of the serum tryptophan level and the depletion of the intestinal microbiota, specifically tryptophan-producing E. coli and Bifidobacterium. These changes are associated with defects in the male mouse social behavior such as a lack of preference towards female bedding in an odor preference test. The addition of fucose to the test animals' diet altered the bacterial community, increased the abundance of tryptophan-producing E. coli, normalized blood tryptophan levels, and ameliorated social behavior deficits. At the same time, we observed no ameliorating effect of fucose on colon morphology and colitis. Our results suggest a possible mechanism by which intestinal inflammation affects social behavior in male mice. We propose fucose as a promising prebiotic, since it creates a favorable environment for the beneficial bacteria that promote normalization of serum tryptophan level and amelioration of the behavioral abnormalities in the odor preference test.

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Section: Metabolite Extraction and Nmr Spectroscopymentioning

confidence: 99%

Section: Metabolite Extraction and Nmr Spectroscopymentioning

confidence: 99%

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

et al. 2020

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“…AH is secreted by the ciliary epithelium and enters the posterior chamber. Then it flows around the lens and through the pupil into the anterior chamber, where it drains out of the eye . The lens does not have its own blood supply, it is nourished by the AH.…”

Section: Introductionmentioning

confidence: 99%

“…It contains electrolytes, oxygen, carbon dioxide, glucose, urea, antioxidants (e.g., glutathione, pyroglutamic acid, citric acid or ascorbic acid), organic solutes, amino acids (AA), fatty acids, acylcarnitines, lipids and others. Metabolomics extended our knowledge about the metabolic composition of aqueous humor . It aims to identify and quantify small molecule metabolites present in a studied organism or biological component (e.g.…”

Section: Introductionmentioning

confidence: 99%

An exploratory LC‐MS‐based metabolomics study reveals differences in aqueous humor composition between diabetic and non‐diabetic patients with cataract

et al. 2018

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Cataract is the leading cause of blindness worldwide. Epidemiological studies revealed up to a fivefold increased prevalence of cataracts in diabetic subjects. Metabolomics is nowadays frequently implemented to understand pathophysiological processes responsible for disease occurrence and progression. It has also been used recently to study the metabolic composition of aqueous humor (AH). AH is a transparent fluid which fills the anterior and posterior chambers of the eye. It supplies nutrients and removes metabolic waste from avascular tissues in the eye. The aim of this study was to use metabolomics to compare the AH of diabetic and non-diabetic patients undergoing cataract surgery. Several antioxidants (methyltetrahydrofolic acid, taurine, niacinamide, xanthine, and uric acid) were found decreased (-22 to -61%, p-value 0.05-0.003) in AH of diabetics. Also amino acids (AA) and derivatives were found decreased (-21 to -36%, p-value 0.05-0.01) while glycosylated AA increased (+75-98%, p-value 0.03-0.009) in this group of patients. Metformin was detected in AH of people taking this drug. To our knowledge, this is the first metabolomics study aiming to assess differences in AH composition between diabetic and non-diabetic patients with cataract. An increased oxidative stress and perturbations in amino acid metabolism in AH may be responsible for earlier cataract onset in diabetic patients.

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“…The spectra of S. lucioperca lens and gill have recently been published 15 by our group. For the majority of metabolites, the identification was performed according to their NMR spectra available in literature 2 and in our in-house library [9][10][11]15,16 without additional confirmation. In some cases, the signal assignment was unobvious; in these cases, the identification was confirmed by spiking the extract with commercial standard compounds.…”

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confidence: 99%

Most abundant metabolites in tissues of freshwater fish pike-perch (Sander lucioperca)

Tsentalovich

Zelentsova

Yanshole

et al. 2020

Sci Rep

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Quantitative metabolomic analysis was performed for eleven tissues of freshwater fish pike-perch (Sander lucioperca), including gill, heart, liver, kidney, spleen, muscle, brain, milt, lens, aqueous (AH) and vitreous (VH) humors with the use of NMR spectroscopy. The absolute values of concentrations were determined for more than 65 most abundant metabolites in every tissue. It was found that from the metabolomic viewpoint, kidney and gill are the most similar tissues, while the metabolomic compositions of ocular tissues—lens, AH, and VH significantly differ from that of other tissues. The combinations of intracellular osmolytes and antioxidants are specific for every tissue. In particular, the concentration of antioxidant ovothiol A in the lens is much higher than in any other tissue, while the brain enjoys the elevated level of ascorbate. The most abundant osmolyte in the fish spleen, muscle, and heart is taurine, and in the brain, gill, and lens—myo-inositol. Other important osmolytes specific for particular tissues are N-acetyl-histidine, N-acetyl-aspartate, betaine, threonine-phosphoethanolamine, and serine-phosphoethanolamine. The quantitative data obtained in the present work can be used as the baseline metabolite concentrations in the fish tissues to evaluate the influence of seasonal, ecological and other factors on the fish metabolism.

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Metabolomics of the human aqueous humor

Cited by 33 publications

References 33 publications

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

Fucose Ameliorates Tryptophan Metabolism and Behavioral Abnormalities in a Mouse Model of Chronic Colitis

An exploratory LC‐MS‐based metabolomics study reveals differences in aqueous humor composition between diabetic and non‐diabetic patients with cataract

Most abundant metabolites in tissues of freshwater fish pike-perch (Sander lucioperca)

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