Metabolomics of temperature stress

Guy, Charles L.; Kaplan, Fatma; Kopka, Joachim; Selbig, Joachim; Hincha, Dirk K.

doi:10.1111/j.1399-3054.2007.00999.x

Cited by 455 publications

(352 citation statements)

References 78 publications

Supporting

Mentioning

314

Contrasting

Unclassified

Order By: Relevance

“…Metabolite fingerprints to cold stress were obtained for Drosophila melanogaster individuals (Korn et al, 2010) and showed that the strategies to cold adaptation might be the same within all members of the kingdom. Janská et al (2011) confirmed all the significant metabolic variations, stirring in cold acclimatization, reenforcing the impression that the combination of cryoprotectant molecules is of great valve (Guy et al, 2008). Amongst these cryo-protectans, sugars, sugar alcohols and proline were exposed to be of higher significance.…”

Section: Metabolomics Profiling In Crop Plantssupporting

confidence: 49%

Advances in detection of stress tolerance in plants through metabolomics approaches

Khan¹,

Ali²,

Shahid³

et al. 2017

Plant Omics

View full text Add to dashboard Cite

Heat and drought stresses are presently the principal risk on world's food quantity, limiting yield. Both of these two stresses affect plants metabolism, physiological and morphological processes, which ultimately reduces the productivity. The plant cell develops different stress induced self-defence mechanisms to reduce the effect of stresses. These defence mechanisms are developed by modifying gene expression pattern, which results in qualitative and quantitative deviations in proteins synthesis, leading to the modulation of certain metabolic and defensive pathways. New metabolic profiling technologies offer a great opportunity for biologist to understand defence mechanism of plants under stress conditions. Metabolomics technologies presently enabled the using of different multi-variate analyses, generated from various hyphenated and chromatographic discovery systems, such as gas or liquid chromatography together with mass spectrometry, or nuclear magnetic resonance (NMR) based methods. Investigation and mining of metabolomics data can be done through a blend of different statistical methods, such as independent component analysis and analysis of variance. Metabolomics in combination with gene expression, protein interaction and other different regulatory pathways can be useful to diverse organisms with trivial alterations. In recent time, this technology has been used to investigate drought tolerance in plant crops to find particular stress related patterns in metabolic expression. These studies identified the vital roles of primary and secondary metabolites associated with abiotic stress tolerance.

show abstract

Section: Metabolomics Profiling In Crop Plantssupporting

confidence: 49%

Advances in detection of stress tolerance in plants through metabolomics approaches

Khan¹,

Ali²,

Shahid³

et al. 2017

Plant Omics

View full text Add to dashboard Cite

show abstract

“…Despite the suitability of this species for studying temperature stress (stress can be applied homogenously and rapidly to all cells, vegetative cells are undifferentiated, the genome is sequenced (82), gene families are smaller compared with higher plants), to our knowledge, there are no studies about cold stress in C. reinhardtii. Former studies in plant biology have described mechanisms implied in the cold response such as metabolomic changes (30,53,(83)(84)(85), integration of metabolomic and proteomic phenotypes (30), anatomical changes, sugar accumulation (15,26), late embryogenesis abundant (LEA), and heat shock (HSP) proteins mediating stabilization of membranes and proteins (65,86), and the activation of C-repeatbinding factor and dehydration responsive element-binding factor 1 (CBF/DREB) responsive pathways (2,9). Most of these studies have been focused on individual mechanisms with comparison of control versus stressed plants.…”

Section: Discussionmentioning

confidence: 99%

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Valledor

Furuhashi

Hanak

et al. 2013

Molecular & Cellular Proteomics

128

131

View full text Add to dashboard Cite

Chlamydomonas reinhardtii is one of the most important model organisms nowadays phylogenetically situated between higher plants and animals (Merchant et al. 2007). Stress adaptation of this unicellular model algae is in the focus because of its relevance to biomass and biofuel production. Here, we have studied cold stress adaptation of C. reinhardtii hitherto not described for this algae whereas intensively studied in higher plants. Toward this goal, high throughput mass spectrometry was employed to integrate proteome, metabolome, physiological and cell-morphological changes during a time-course from 0 to 120 h. These data were complemented with RT-qPCR for target genes involved in central metabolism, signaling, and lipid biosynthesis. Using this approach dynamics in central metabolism were linked to cold-stress dependent sugar and autophagy pathways as well as novel genes in C. reinhardtii such as CKIN1, CKIN2 and a hitherto functionally not annotated protein named CKIN3. Cold stress affected extensively the physiology and the organization of the cell. Gluconeogenesis and starch biosynthesis pathways are activated leading to a pronounced starch and sugar accumulation. Quantitative lipid profiles indicate a sharp decrease in the lipophilic fraction and an increase in polyunsaturated fatty acids suggesting this as a mechanism of maintaining membrane fluidity. The proteome is completely remodeled during cold stress: specific candidates of the ribosome and the spliceosome indicate altered biosynthesis and degradation of proteins important for adaptation to low temperatures. Specific proteasome degradation may be mediated by the observed cold-specific changes in the ubiquitinylation system. Sparse partial least squares regression analysis was applied for protein correlation network analysis using proteins as predictors and Fv/Fm, FW, total lipids, and starch as responses. We applied also Granger causality analysis and revealed correlations between proteins and metabolites otherwise not detectable. Twenty percent of the proteins responsive to cold are uncharacterized proteins. This presents a considerable resource for new discoveries in cold stress biology in alga and plants. Molecular &

show abstract

“…When winter rye is grown at warm temperature, plants are killed upon freezing at about −5°C, but upon exposure to low nonfreezing temperatures, they can survive freezing below −20°C. The molecular basis for this phenomenon, known as cold acclimation, is not completely understood, but includes changes in membrane cryobehavior, the production of cryoprotective proteins, and the biosynthesis of low molecular weight cryoprotectants such as sucrose, raffinose, and proline (2,3).…”

mentioning

confidence: 99%

Photoperiodic regulation of the C-repeat binding factor (CBF) cold acclimation pathway and freezing tolerance inArabidopsis thaliana

Lee

Thomashow

2012

Proc. Natl. Acad. Sci. U.S.A.

298

246

View full text Add to dashboard Cite

The CBF (C-repeat binding factor) pathway has a major role in plant cold acclimation, the process whereby certain plants increase in freezing tolerance in response to low nonfreezing temperatures. In Arabidopsis thaliana, the pathway is characterized by rapid cold induction of CBF1, CBF2, and CBF3, which encode transcriptional activators, followed by induction of CBF-targeted genes that impart freezing tolerance. At warm temperatures, CBF transcript levels are low, but oscillate due to circadian regulation with peak expression occurring at 8 h after dawn (zeitgeber time 8; ZT8). Here, we establish that the CBF pathway is also regulated by photoperiod at warm temperatures. At ZT8, CBF transcript levels in short-day (SD; 8-h photoperiod) plants were three-to fivefold higher than in long-day plants (LD; 16-h photoperiod). Moreover, the freezing tolerance of SD plants was greater than that of LD plants. Genetic analysis indicated that phytochrome B (PHYB) and two phytochrome-interacting factors, PIF4 and PIF7, act to down-regulate the CBF pathway and freezing tolerance under LD conditions. Down-regulation of the CBF pathway in LD plants correlated with higher PIF4 and PIF7 transcript levels and greater stability of the PIF4 and PIF7 proteins under LD conditions. Our results indicate that during the warm LD growing season, the CBF pathway is actively repressed by PHYB, PIF4, and PIF7, thus mitigating allocation of energy and nutrient resources toward unneeded frost protection. This repression is relieved by shortening day length resulting in up-regulation of the CBF pathway and increased freezing tolerance in preparation for coming cold temperatures.

show abstract

Metabolomics of temperature stress

Cited by 455 publications

References 78 publications

Advances in detection of stress tolerance in plants through metabolomics approaches

Advances in detection of stress tolerance in plants through metabolomics approaches

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Photoperiodic regulation of the C-repeat binding factor (CBF) cold acclimation pathway and freezing tolerance inArabidopsis thaliana

Contact Info

Product

Resources

About