Metabolomics in Cardiovascular Disease: Towards Clinical Application

Barba, Ignasi; Garcı́a-Dorado, David

doi:10.5772/30677

Cited by 4 publications

(5 citation statements)

References 63 publications

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“…Metabolic effects of low-dose ionizing radiation on the heart cells have not been studied yet. However, many reports indicated that metabolic alterations occur in other human cells irradiated with low or high doses as well as under various pathological conditions (Kell et al, 2005;Barba & Garcia-Dorado, 2012;Laiakis et al, 2012;Goudarzi et al, 2014;Kwon et al, 2014;Jang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Our results seem to be consistent with such statement -the NMR analysis enabled us to detect the metabolic changes in irradiated cells, while standard biological tests revealed to be less sensitive. Thus, NMR opens new possibilities for monitoring changes in metabolites associated with heart damage, and what is more important, for identifying the metabolites that change depending on the toxic agent applied (Barba & Garcia-Dorado, 2012). Several studies showed that various cardiac disorders are represented by different metabolic biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…The levels of some metabolites are altered by various pathological states and the changes in the metabolic profile can be observed long before the clinical symptoms develop. What is more important, different metabolites may change depending on the toxic agent (Barba & Garcia-Dorado, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

Gramatyka¹,

Skorupa²,

Sokół³

2018

Acta Biochim Pol

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Several lines of evidence indicate that exposure of heart to ionizing radiation increases the risk of cardiotoxicity manifested by heart dysfunction and cardiovascular diseases. It was initially believed that the heart is an organ relatively resistant to radiation. Currently, however, it is suspected that even low doses of radiation (< 2 Gy) may have a negative impact on the cardiovascular system. Cardiotoxicity of ionizing radiation is associated with metabolic changes observed in cardiac cells injured by radiation. In this study, we used human cardiomyocytes as a model system, and studied their metabolic response to radiation using high-resolution magic angle spinning nuclear magnetic resonance techniques (HR-MAS NMR). Human cardiomyocytes cultured in vitro were exposed to ionizing radiation and their survival was assessed by clonogenic assay. Changes in apoptosis intensity and cell cycle distribution after the irradiation were measured as well. NMR spectra of cardiomyocytes were acquired using Bruker Avance 400 MHz spectrometer at a spinning rate of 3200 Hz. Survival of cardiomyocytes after NMR experiments was assessed by the Trypan blue exclusion assay. Exposure of cardiomyocytes to small doses of ionizing radiation had no effect on cell proliferation potential and intensity of cell death. However, analysis of metabolic profiles revealed changes in lipids, threonine, glycine, glycerophosphocholine, choline, valine, isoleucine, glutamate, reduced glutathione and taurine metabolism. The results of this study showed that ionizing radiation affects metabolic profiles of cardiomyocytes even at low doses, which potentially have no effect on cell viability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

Gramatyka¹,

Skorupa²,

Sokół³

2018

Acta Biochim Pol

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“…Metabolomics is a technique that allows the identification and quantitative measurement of metabolites in biological systems. It is one of the most effective methods to detect cellular response to various endogenous and exogenous (environmental) factors, including ionizing radiation [39]. Several metabolomics studies showed that even putatively harmless doses of radiation (up to 2 Gy) induce molecular changes that affect the metabolic profile.…”

Section: Metabolomics and Radiation-induced Changes In Cellular Metab...mentioning

confidence: 99%

The Radioprotective Activity of Resveratrol—Metabolomic Point of View

Gramatyka

2022

Metabolites

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Resveratrol, a plant-derived polyphenol, is an intensively studied compound with widely documented positive effects on health. Antioxidant activity is the property most often mentioned as responsible for its beneficial effects. Therefore, since the adverse effect of ionizing radiation is primarily related to the induction of oxidative stress, the question arises of whether the use of resveratrol could have a radioprotective effect. This paper summarizes the data on the cytoprotective activity of resveratrol and pieces of evidence for the potential interplay between response to radiation and resveratrol activity. The paper focuses on changes in the metabolic profile of cells and organisms induced by ionizing radiation and exposure to resveratrol. The comparison of metabolic changes induced by both factors provides a rationale for the potential mechanism of the radioprotective effects of resveratrol.

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“…Metabolomics, a subfield in molecular profiling, investigates the metabolome, the total quantitative collection of small-molecule metabolites in biofluids such as plasma [ 1 , 2 ], in an identified and quantified manner [ 3 ]. Since metabolomic changes are downstream of alterations at the genomic, transcriptomic and proteomic level [ 4 ], metabolomics is particularly suited for identification of biomarkers, examination of molecular physiology, and investigation of genetic and environmental modifications [ 5 ]. Commonly utilized technologies in metabolomics include liquid or gas chromatography (LC and GC) coupled to mass spectrometry (MS), capillary electrophoresis (CE), and nuclear magnetic resonance (NMR) spectroscopy [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Recommendations for Improving Identification and Quantification in Non-Targeted, GC-MS-Based Metabolomic Profiling of Human Plasma

et al. 2017

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The field of metabolomics as applied to human disease and health is rapidly expanding. In recent efforts of metabolomics research, greater emphasis has been placed on quality control and method validation. In this study, we report an experience with quality control and a practical application of method validation. Specifically, we sought to identify and modify steps in gas chromatography-mass spectrometry (GC-MS)-based, non-targeted metabolomic profiling of human plasma that could influence metabolite identification and quantification. Our experimental design included two studies: (1) a limiting-dilution study, which investigated the effects of dilution on analyte identification and quantification; and (2) a concentration-specific study, which compared the optimal plasma extract volume established in the first study with the volume used in the current institutional protocol. We confirmed that contaminants, concentration, repeatability and intermediate precision are major factors influencing metabolite identification and quantification. In addition, we established methods for improved metabolite identification and quantification, which were summarized to provide recommendations for experimental design of GC-MS-based non-targeted profiling of human plasma.

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Metabolomics in Cardiovascular Disease: Towards Clinical Application

Cited by 4 publications

References 63 publications

Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

Nuclear magnetic resonance spectroscopy reveals metabolic changes in living cardiomyocytes after low doses of ionizing radiation

The Radioprotective Activity of Resveratrol—Metabolomic Point of View

Recommendations for Improving Identification and Quantification in Non-Targeted, GC-MS-Based Metabolomic Profiling of Human Plasma

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