Metabolomics in Antimicrobial Drug Discovery: The Success of the Chemical Diversity

Bueno, Juan

doi:10.4172/1948-5948.1000242

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…Nevertheless, flux balance analyses that rely on steady-state assumption provide significant insights into the dynamics of metabolism (Filipp 2013;Kumar et al 2015a). These analyses are enabled by monitoring of the abundance of metabolites via NMR and MS, as well as monitoring of the abundance and functional activity of enzymes through transcriptomic and proteomic screening for decoding of complex agricultural traits (Joshi et al 2002;Filipp 2013;Babu et al 2012;Gong et al 2013;Agrawal et al 2013;Seifert et al 2013;Wen et al 2014;Caspi et al 2013;Schweiger et al 2014;Bueno 2015).…”

Section: Bio-prospecting and Metabolomicsmentioning

confidence: 99%

Augmentation of crop productivity through interventions of omics technologies in India: challenges and opportunities

et al. 2018

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With the continuous increase in the population of developing countries and decline of natural resources, there is an urgent need to qualitatively and quantitatively augment crop productivity by using new tools and technologies for improvement of agriculturally important traits. The new scientific and technological omics-based approaches have enabled us to deal with several issues and challenges faced by modern agricultural system and provided us novel opportunities for ensuring food and nutritional security. Recent developments in sequencing techniques have made available huge amount of genomic and transcriptomic data on model and cultivated crop plants including Arabidopsis thaliana, Oryza sativa, Triticum aestivum etc. The sequencing data along with other data generated through several omics platforms have significantly influenced the disciplines of crop sciences. Gene discovery and expression profiling-based technologies are offering enormous opportunities to the scientific community which can now apply marker-assisted selection technology to assess and enhance diversity in their collected germplasm, introgress essential traits from new sources and investigate genes that control key traits of crop plants. Utilization of omics science and technologies for crop productivity, protection and management has recently been receiving a lot of attention; the majority of the efforts have been put into signifying the possible applications of various omics technologies in crop plant sciences. This article highlights the background of challenges and opportunities for augmentation of crop productivity through interventions of omics technologies in India.

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Section: Bio-prospecting and Metabolomicsmentioning

confidence: 99%

Augmentation of crop productivity through interventions of omics technologies in India: challenges and opportunities

et al. 2018

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“…Finalmente, la metabolómica es altamente relevante para estudiar la biodiversidad en términos de sus metabolitos naturales bioactivos, contribuyendo a la bioprospección con miras a lograr un desarrollo sostenible de la región. La metabolómica puede utilizarse como herramienta de tamizaje para priorizar especies promisorias para la producción de metabolitos con propiedades de interés (Cox et al, 2014), en la búsqueda de antioxidantes (Cuadrado-Silva et al, 2016), y para el descubrimiento de drogas antimicrobianas a partir de la diversidad química de ciertas especies de microorganismos (Bueno 2015).…”

Section: Metabolómicaunclassified

Biodiversidad latinoamericana y sus perspectivas de estudio con tecnologías ‘ómicas’

Garavito¹,

Muñoz²,

Rendón³

et al. 2017

Mexjbiotechnol

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Latinoamérica es una de las regiones del mundo más ricas en biodiversidad. Los países de la región albergan más del 70% de las especies del planeta, en un territorio que no alcanza a ser el 10% de la biósfera terrestre. En esta revisión discutiremos la disponibilidad de nuevas técnicas para el análisis masivo de esta biodiversidad, a través del uso de las tecnologías ‘ómicas’ y los correspondientes análisis bioinformáticos de los datos producidos con estas tecnologías. Como nunca antes, los científicos cuentan con herramientas para el estudio del ADN que les permitan encontrar en los organismos de nuestra diversidad genes relacionados con mejoras en productividad en cultivos, rutas de producción de compuestos de interés industrial, resiliencia de las especies al cambio climático y los genes de adaptabilidad a estrés bióticos y abióticos, entre otras aplicaciones. Aunque existen en Latinoamérica algunos avances en la secuenciación y análisis de genomas de ciertos grupos de organismos, es indispensable que la región desarrolle proyectos con alianzas entre países para acelerar hallazgos científicos e incluir estudios en las nuevas tecnologías 'ómicas' y bioinformáticas para el análisis masivo de las miles de especies sin estudiar que aún tenemos en nuestros territorios.

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“…Metabolomic reprogramming refers to the ability of cells to alter their metabolome and phenotypic characteristics in response to environmental changes [ 1 , 2 ]. Metabolites are intermediate molecules and by-products of metabolic catalysis [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Exposure of Helicobacter pylori to clarithromycin in vitro resulting in the development of resistance and triggers metabolic reprogramming associated with virulence and pathogenicity

Rosli,

Al-Maleki,

Loke

et al. 2024

PLoS ONE

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In H. pylori infection, antibiotic-resistance is one of the most common causes of treatment failure. Bacterial metabolic activities, such as energy production, bacterial growth, cell wall construction, and cell-cell communication, all play important roles in antimicrobial resistance mechanisms. Identification of microbial metabolites may result in the discovery of novel antimicrobial therapeutic targets and treatments. The purpose of this work is to assess H. pylori metabolomic reprogramming in order to reveal the underlying mechanisms associated with the development of clarithromycin resistance. Previously, four H. pylori isolates were induced to become resistant to clarithromycin in vitro by incrementally increasing the concentrations of clarithromycin. Bacterial metabolites were extracted using the Bligh and Dyer technique and analyzed using metabolomic fingerprinting based on Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (LC-Q-ToF-MS). The data was processed and analyzed using the MassHunter Qualitative Analysis and Mass Profiler Professional software. In parental sensitivity (S), breakpoint isolates (B), and induced resistance isolates (R) H. pylori isolates, 982 metabolites were found. Furthermore, based on accurate mass, isotope ratios, abundances, and spacing, 292 metabolites matched the metabolites in the Agilent METLIN precise Mass-Personal Metabolite Database and Library (AM-PCDL). Several metabolites associated with bacterial virulence, pathogenicity, survival, and proliferation (L-leucine, Pyridoxone [Vitamine B6], D-Mannitol, Sphingolipids, Indoleacrylic acid, Dulcitol, and D-Proline) were found to be elevated in generated resistant H. pylori isolates when compared to parental sensitive isolates. The elevated metabolites could be part of antibiotics resistance mechanisms. Understanding the fundamental metabolome changes in the course of progressing from clarithromycin-sensitive to breakpoint to resistant in H. pylori clinical isolates may be a promising strategy for discovering novel alternatives therapeutic targets.

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Metabolomics in Antimicrobial Drug Discovery: The Success of the Chemical Diversity

Cited by 6 publications

References 47 publications

Augmentation of crop productivity through interventions of omics technologies in India: challenges and opportunities

Augmentation of crop productivity through interventions of omics technologies in India: challenges and opportunities

Biodiversidad latinoamericana y sus perspectivas de estudio con tecnologías ‘ómicas’

Exposure of Helicobacter pylori to clarithromycin in vitro resulting in the development of resistance and triggers metabolic reprogramming associated with virulence and pathogenicity

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