Metabolomics combined with physiology and transcriptomics reveal key metabolic pathway responses in apple plants exposure to different selenium concentrations

Liu, Chunling; Zhou, Guangjin; Qin, Hanhan; Guan, Yafei; Wang, Tianyu; Ni, Wei; Xie, Hongmei; Xing, Yue; Tian, Ge; Lyu, Mengxue; Liu, Jingquan; Wang, Fen; Xu, Xinxiang; Zhu, Zhanling; Jiang, Yuanmao; Ge, Shunfeng

doi:10.1016/j.jhazmat.2023.132953

Cited by 13 publications

(6 citation statements)

References 62 publications

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“…The third level suggests that high doses of Se can have harmful consequences [ 52 , 53 ]. For example, low Se concentrations significantly promote the growth of apple ( Malus pumila ) plants, but once the Se concentration exceeds 12 μM, plant growth is notably inhibited, particularly in the roots [ 54 ]. High concentrations of Se in plants can induce phytotoxicity by interfering with metabolism, resulting in chlorosis, cell membrane degradation, senescence, and reduced grain yield [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

High Concentrations of Se Inhibited the Growth of Rice Seedlings

Liu,

Ma,

et al. 2024

Plants

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Selenium (Se) is crucial for both plants and humans, with plants acting as the main source for human Se intake. In plants, moderate Se enhances growth and increases stress resistance, whereas excessive Se leads to toxicity. The physiological mechanisms by which Se influences rice seedlings’ growth are poorly understood and require additional research. In order to study the effects of selenium stress on rice seedlings, plant phenotype analysis, root scanning, metal ion content determination, physiological response index determination, hormone level determination, quantitative PCR (qPCR), and other methods were used. Our findings indicated that sodium selenite had dual effects on rice seedling growth under hydroponic conditions. At low concentrations, Se treatment promotes rice seedling growth by enhancing biomass, root length, and antioxidant capacity. Conversely, high concentrations of sodium selenite impair and damage rice, as evidenced by leaf yellowing, reduced chlorophyll content, decreased biomass, and stunted growth. Elevated Se levels also significantly affect antioxidase activities and the levels of proline, malondialdehyde, metal ions, and various phytohormones and selenium metabolism, ion transport, and antioxidant genes in rice. The adverse effects of high Se concentrations may directly disrupt protein synthesis or indirectly induce oxidative stress by altering the absorption and synthesis of other compounds. This study aims to elucidate the physiological responses of rice to Se toxicity stress and lay the groundwork for the development of Se-enriched rice varieties.

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Section: Discussionmentioning

confidence: 99%

High Concentrations of Se Inhibited the Growth of Rice Seedlings

Liu,

Ma,

et al. 2024

Plants

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“…Root activity was measured using the triphenyltetrazolium chloride (TTC) method, and the reduction strength of tetrazolium (μg h −1 g −1 FW) was used to express root activity [ 31 ]. WinRhizo software (WinRHIZO Pro 2017a, Regent Instruments Canada) was used to analyze root morphological index [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Application Timing and Levels Affect the Fate and Budget of Fertilizer Nitrogen in the Apple–Soil System

Wang,

Yang

et al. 2024

Plants

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This study aimed to determine the effects of the nitrogen (N) application period and level on the fate of fertilizer N and the contribution of N absorption and translocation to apple organ N. Two N application periods (labeled by the 15N tracer technique in spring and summer, represented by SP and SU, respectively) and three N levels (N0, MN, and HN) were used to determine the physiological indexes and aboveground, root, and soil 15N content of 4-year-old dwarf (‘Red Fuji’/M9T337) and arborized (‘Red Fuji’/Malus hupehensis Rehd.) apple trees. The results showed that HN led to shoot overgrowth, which was not conducive to the growth of the apple root system (root length, root tips, root surface area, and root volume) or the improvement of root activity. The contribution of soil N to apple organ N accounted for more than 50%, and the contribution of N application in summer to fruit N was higher than that in spring. Under HN treatment, the proportion of soil N absorbed by trees decreased, while that of fertilizer N increased; however, the highest proportion was still less than 50%, so apple trees were highly dependent on soil N. Under MN treatment, fertilizer N residue was similar to soil N consumption, and soil N fertility maintained a basic balance. Under HN treatment, fertilizer N residue was significantly higher than soil N consumption, indicating that excessive N application increased fertilizer N residue in the soil. Overall, the 15N utilization rate of arborized trees (17.33–22.38%) was higher than that of dwarf trees (12.89–16.91%). A total of 12.89–22.38% of fertilizer 15N was absorbed by trees, 30.37–35.41% of fertilizer 15N remained in the soil, and 44.65–54.46% of fertilizer 15N was lost. The 15N utilization rate and 15N residual rate of summer N application were higher than those of spring N application, and the 15N loss rate was lower than that of spring N application. High microbial biomass N (MBN) may be one of the reasons for the high N utilization rate and the low loss rate of N application in summer.

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“…CAT activity was determined by monitoring the decomposition of H 2 O 2 at 240 nm for 3 min with a slight modification [ 88 , 89 ]. The reaction mixture consisted of 2 mL of 50 mmol/L sodium phosphate buffer (pH 7.0), 1 mL of 0.2% H 2 O 2 , and 0.2 mL of enzyme extract.…”

Section: Methodsmentioning

confidence: 99%

“…FPKM values were used to indicate transcript or gene expression levels. The specific steps of data processing were carried out as described by Meng et al [ 88 , 89 ].…”

Section: Methodsmentioning

confidence: 99%

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Metabolomics and transcriptomics combined with physiology reveal key metabolic pathway responses in tobacco roots exposed to NaHS

Yang,

Wen,

Yang

et al. 2024

BMC Plant Biol

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Hydrogen sulfide (H2S) has emerged as a novel endogenous gas signaling molecule, joining the ranks of nitric oxide (NO) and carbon monoxide (CO). Recent research has highlighted its involvement in various physiological processes, such as promoting root organogenesis, regulating stomatal movement and photosynthesis, and enhancing plant growth, development, and stress resistance. Tobacco, a significant cash crop crucial for farmers’ economic income, relies heavily on root development to affect leaf growth, disease resistance, chemical composition, and yield. Despite its importance, there remains a scarcity of studies investigating the role of H2S in promoting tobacco growth. This study exposed tobacco seedlings to different concentrations of NaHS (an exogenous H2S donor) − 0, 200, 400, 600, and 800 mg/L. Results indicated a positive correlation between NaHS concentration and root length, wet weight, root activity, and antioxidant enzymatic activities (CAT, SOD, and POD) in tobacco roots. Transcriptomic and metabolomic analyses revealed that treatment with 600 mg/L NaHS significantly effected 162 key genes, 44 key enzymes, and two metabolic pathways (brassinosteroid synthesis and aspartate biosynthesis) in tobacco seedlings. The addition of exogenous NaHS not only promoted tobacco root development but also potentially reduced pesticide usage, contributing to a more sustainable ecological environment. Overall, this study sheds light on the primary metabolic pathways involved in tobacco root response to NaHS, offering new genetic insights for future investigations into plant root development.

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Metabolomics combined with physiology and transcriptomics reveal key metabolic pathway responses in apple plants exposure to different selenium concentrations

Cited by 13 publications

References 62 publications

High Concentrations of Se Inhibited the Growth of Rice Seedlings

High Concentrations of Se Inhibited the Growth of Rice Seedlings

Nitrogen Application Timing and Levels Affect the Fate and Budget of Fertilizer Nitrogen in the Apple–Soil System

Metabolomics and transcriptomics combined with physiology reveal key metabolic pathway responses in tobacco roots exposed to NaHS

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