Metabolomics as a valid analytical technique in environmental exposure research: application and progress

Wei, Shuang; Wei, Yuanyun; Gong, Yaqi; Chen, Yonglin; Cui, Jian; Li, Linwei; Yan, Hongxia; Yu, Yueqiu; Lin, Xiang; Li, Guoqing; Liang, Yi

doi:10.1007/s11306-022-01895-7

Cited by 18 publications

(9 citation statements)

References 150 publications

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“…The application of metabolomic technology to examine the overall metabolite groups of herbs and study the metabolic phenotypes of herbal germplasm resources will provide a scientific basis for the sustainable development and utilization of various herbal resources (Duan et al, 2018; Jiang et al, 2021; Shen et al, 2022; Zeng et al, 2022). It is of theoretical significance and practical value to ensure the rational, safe and effective use of traditional Chinese medicine with modern scientific methods and standards for understanding it (Wang et al, 2017, 2021; Wei et al, 2022). In this study, the metabolites and metabolic pathways of WTP were hypothesized using metabolomics technology, which can help further improve our understanding of the pharmacological and toxic effects and the material basis of these pharmacological effects, and optimize drug design from S. chinensis .…”

Section: Discussionmentioning

confidence: 99%

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Characterization of chemical constituents and metabolites in vivo and in vitro after oral administration of Wuteng tablets in rats by UHPLC–Q/TOF–MS

Chen

Pang

et al. 2023

Biomedical Chromatography

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Waste medicinal plants are widely used in drug production. With the increasing demand for botanical drugs, there is an urgent need to identify new and effective drugs and improve the utilization of medicinal plant resources. Wuteng tablets (WTP) are extracted from the stem of Schisandra chinensis and have a good therapeutic effect on Alzheimer’s disease. In this study, a holistic identification strategy based on UHPLC–Q/TOF–MS was developed for the first time to investigate the metabolites and metabolic pathways involved in the in vitro metabolism and liver microsomal incubation and in the in vivo metabolic system of rats after WTP administration. After the oral administration of WTP, 21 metabolites were identified in the serum and 25 metabolites were identified in the urine, of which six were new metabolites; 33 metabolites were inferred from the microsomal metabolites in vitro. The metabolic pathways related to WTP mainly involve demethylation, hydroxylation, dehydroxylation and dehydrogenation. In this study, the metabolites and metabolic pathways of WTP were elucidated via UHPLC–Q/TOF–MS, which provided a basis for an in‐depth study of the pharmacodynamic and pharmacotoxicological effects of WTP.

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Section: Discussionmentioning

confidence: 99%

“…understanding it (Wang et al, 2017(Wang et al, , 2021Wei et al, 2022). In this study, the metabolites and metabolic pathways of WTP were…”

Section: In Vitro Metabolism Analysis Of Liver Microsomesmentioning

confidence: 96%

Characterization of chemical constituents and metabolites in vivo and in vitro after oral administration of Wuteng tablets in rats by UHPLC–Q/TOF–MS

Chen

Pang

et al. 2023

Biomedical Chromatography

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“…Metabolic homeostasis is intricately regulated and sensitive to environmental conditions, and metabolites can provide insight into the body’s dynamic response to diet, exercise, pharmaceutical use, and exposure to chemical stressors 60 . Metabolic profiling can, for example, provide signatures for and identify responses to environmental exposures as varied as air pollution, persistent organic pollutants, proximity to industrial operations, metals, perfluorinated substances, plasticizers, and climate-related variables 61 – 63 .…”

Section: Introductionmentioning

confidence: 99%

A precision environmental health approach to prevention of human disease

2023

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Human health is determined by the interaction of our environment with the genome, epigenome, and microbiome, which shape the transcriptomic, proteomic, and metabolomic landscape of cells and tissues. Precision environmental health is an emerging field leveraging environmental and system-level (‘omic) data to understand underlying environmental causes of disease, identify biomarkers of exposure and response, and develop new prevention and intervention strategies. In this article we provide real-life illustrations of the utility of precision environmental health approaches, identify current challenges in the field, and outline new opportunities to promote health through a precision environmental health framework.

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“…Metabolomics has the ability to circumvent the limitations of other MBTs by providing direct information about metabolic activity, and an increasing number of metabolomics studies in clinical ( Gonzalez-Covarrubias et al, 2022 ), toxicological ( Morgan et al, 2022 ), and environmental ( Wei et al, 2022 ) settings demonstrated the value of this approach ( Pang et al, 2021 ). Bioremediation of chlorinated ethenes is also often hindered by the process stalling, which leaves behind either cis -1,2-dichloroethene (cDCE) or vinyl chloride ( Lihl et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolome patterns identify active dechlorination in bioaugmentation consortium SDC-9™

May

Xie²,

Murdoch³

et al. 2022

Front. Microbiol.

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Ultra-high performance liquid chromatography–high-resolution mass spectrometry (UPHLC–HRMS) is used to discover and monitor single or sets of biomarkers informing about metabolic processes of interest. The technique can detect 1000’s of molecules (i.e., metabolites) in a single instrument run and provide a measurement of the global metabolome, which could be a fingerprint of activity. Despite the power of this approach, technical challenges have hindered the effective use of metabolomics to interrogate microbial communities implicated in the removal of priority contaminants. Herein, our efforts to circumvent these challenges and apply this emerging systems biology technique to microbiomes relevant for contaminant biodegradation will be discussed. Chlorinated ethenes impact many contaminated sites, and detoxification can be achieved by organohalide-respiring bacteria, a process currently assessed by quantitative gene-centric tools (e.g., quantitative PCR). This laboratory study monitored the metabolome of the SDC-9™ bioaugmentation consortium during cis-1,2-dichloroethene (cDCE) conversion to vinyl chloride (VC) and nontoxic ethene. Untargeted metabolomics using an UHPLC-Orbitrap mass spectrometer and performed on SDC-9™ cultures at different stages of the reductive dechlorination process detected ~10,000 spectral features per sample arising from water-soluble molecules with both known and unknown structures. Multivariate statistical techniques including partial least squares-discriminate analysis (PLSDA) identified patterns of measurable spectral features (peak patterns) that correlated with dechlorination (in)activity, and ANOVA analyses identified 18 potential biomarkers for this process. Statistical clustering of samples with these 18 features identified dechlorination activity more reliably than clustering of samples based only on chlorinated ethene concentration and Dhc 16S rRNA gene abundance data, highlighting the potential value of metabolomic workflows as an innovative site assessment and bioremediation monitoring tool.

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Metabolomics as a valid analytical technique in environmental exposure research: application and progress

Cited by 18 publications

References 150 publications

Characterization of chemical constituents and metabolites in vivo and in vitro after oral administration of Wuteng tablets in rats by UHPLC–Q/TOF–MS

Characterization of chemical constituents and metabolites in vivo and in vitro after oral administration of Wuteng tablets in rats by UHPLC–Q/TOF–MS

A precision environmental health approach to prevention of human disease

Metabolome patterns identify active dechlorination in bioaugmentation consortium SDC-9™

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