Metabolomics approach for understanding geographical dependence of soybean leaf metabolome

Yun, Dae-Yong; Kang, Young‐Gyu; Kim, Eun‐Hee; Kim, Myoyeon; Park, Nok-Hyun; Choi, Heekyung; Go, Gun Hee; Lee, John Hwan; Park, Jun Seong; Hong, Young-Shick

doi:10.1016/j.foodres.2018.01.061

Cited by 31 publications

(19 citation statements)

References 41 publications

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“…They found a decreased production of chlorophyll-related metabolites and a higher level of disaccharides from summer to autumn. Another metabolomic approach analyzed soy leaves from crops with different geographical localizations and identified different amounts of metabolites such as pinitol and flavonoids [96]. An excellent review performed by Feng et al [97] summarizes the use of metabolomics in soy under abiotic stress.…”

Section: Metabolomics and Soy An Overviewmentioning

confidence: 99%

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Bragagnolo

Funari

Ibáñez

2021

Foods

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The valorization of agri-food by-products is essential from both economic and sustainability perspectives. The large quantity of such materials causes problems for the environment; however, they can also generate new valuable ingredients and products which promote beneficial effects on human health. It is estimated that soybean production, the major oilseed crop worldwide, will leave about 597 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2020/21. An alternative for the use of soy-related by-products arises from the several bioactive compounds found in this plant. Metabolomics studies have already identified isoflavonoids, saponins, and organic and fatty acids, among other metabolites, in all soy organs. The present review aims to show the application of metabolomics for identifying high-added-value compounds in underused parts of the soy plant, listing the main bioactive metabolites identified up to now, as well as the factors affecting their production.

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Section: Metabolomics and Soy An Overviewmentioning

confidence: 99%

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Bragagnolo

Funari

Ibáñez

2021

Foods

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“…Nuclear magnetic resonance (NMR) is worldwide well-stablished spectroscopic technique that allows to obtain information related to the genotype, phenotype, and intra- and interorganism classifications based on its origin and biological importance, environmental toxicity, and pollution [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. In such, NMR spectroscopy is widely used in multidisciplinary “omics”, such as metabolomics, metabolic profiling, fingerprinting, and phenotyping [ 11 , 12 , 13 , 14 ], as well as in identification and structural determination of organic compounds in various samples such as food [ 4 ], ice [ 8 ], serum [ 10 ], environmental [ 15 ], material science [ 16 ], and water [ 17 ]. In addition, high-resolution magic angle spinning (HR-MAS) is a multipurpose NMR tool allowing the acquisition of NMR data directly from semi-solid (i.e., gel-like) materials (e.g., plant tissues) in their natural, unaltered states, without laborious sample preparation steps, and then preventing changes in the chemical composition during these process [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Magic Angle Spinning (HR-MAS) NMR-Based Fingerprints Determination in the Medicinal Plant Berberis laurina

et al. 2020

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Berberis laurina (Berberidaceae) is a well-known medicinal plant used in traditional medicine since ancient times; however, it is scarcely studied to a large-scale fingerprint. This work presents a broad-range fingerprints determination through high-resolution magical angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy, a well-established flexible analytical method and one of most powerful “omics” platforms. It had been intended to describe a large range of chemical compositions in all plant parts. Beyond that, HR-MAS NMR allowed the direct investigation of botanical material (leaves, stems, and roots) in their natural, unaltered states, preventing molecular changes. The study revealed 17 metabolites, including caffeic acid, and berberine, a remarkable alkaloid from the genus Berberis L. The metabolic pattern changes of the leaves in the course of time were found to be seasonally dependent, probably due to the variability of seasonal and environmental trends. This metabolites overview is of great importance in understanding plant (bio)chemistry and mediating plant survival and is influenceable by interacting environmental means. Moreover, the study will be helpful in medicinal purposes, health sciences, crop evaluations, and genetic and biotechnological research.

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“…Thus, the influence of plant age and genotype on the chemical composition of xylem sap has been overlooked, as only few studies have reported this interaction in leaves and roots. Nevertheless, some metabolomic studies have determined the existence of some differentiation of chemical compound dynamics during different plant growth stages in potato [61], soybeans [62], ginseng [63], and others herbaceous plants [64,65] that may have contributed to different biological activity of the plant, such as flowering period, or might be the result of variations on soil properties or environmental conditions [61,62]. Other studies have determined the effect of plant genotype on sap composition including birch trees [66], mango [67], maize [68] and grapevines [69].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic, Ionomic and Microbial Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

et al. 2021

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Vascular pathogens are the causal agents of main diseases threatening the health and growth of olive crops worldwide. The use of endophytic microorganisms represents a challenging and promising strategy for management of vascular diseases in olive. Although current research has been focused on analyzing the structure and diversity of the endophytic microbial communities inhabiting the olive xylem, the characterization of this ecological niche has been overlooked and to date remain unexplored, despite that the characterization of the xylem sap composition is essential to unravel the nutritional requirements of xylem-limited microorganisms. In this study, branches from plantlets and adult olive trees of cultivars Picual and Arbequina were selected to characterize the chemical and microbial composition of olive xylem sap extracted using a Scholander pressure chamber. Metabolome and ionome analyses of xylem sap were performed by proton nuclear magnetic resonance (NMR) spectroscopy-based and by inductively coupled plasma with optical emission spectroscopy (ICP-OES), respectively. Olive xylem sap metabolites included a higher relative percentage of sugars (54.35%), followed by alcohols (28.85%), amino acids (8.01%), organic acids (7.68%), and osmolytes (1.12%). Within each of these groups, the main metabolites in the olive xylem sap were mannitol, ethanol, glutamine, acetic acid, and trigonelline, whereas K and Cl− were the main element and inorganic anion, respectively. Metabolomic profile varied when comparing olive plant age and genotype. The levels of glucose, fructose, sucrose and mannitol, choline, B and PO43− were significantly higher in adult trees than in plantlets for both olive genotypes, whereas NO3− and Rb content showed the opposite behavior. On the other hand, levels of aspartic acid, phenylalanine, and Na were significantly higher in ‘Picual’ than in ‘Arbequina’, whereas Fe showed the opposite behavior, but only for adult trees. Microbiome composition identified Firmicutes (67%), Proteobacteria (22%) and Actinobacteriota (11%) as the main phyla, while at the genus level Anoxybacillus (52%), Cutibacterium (7%), Massilia (6%), and Pseudomonas (3%) were the most representative. Both non-supervised hierarchical clustering analysis and supervised PLS-DA analysis differentiated xylem sap chemical and microbial composition first, according to the age of the plant and then by the olive genotype. PLS-DA analysis revealed that B, ethanol, Fe, fructose, glucose, mannitol, sucrose, and Sr, and Anoxybacillus, Cutibacterium, and Bradyrhizobium were the most significant chemical compounds and bacterial genera, respectively, in the discrimination of adult olive trees and plantlets. Knowledge of the chemical composition of xylem sap will lead to a better understanding of the complex nutritional requirements of olive xylem-inhabiting microorganisms, including vascular pathogens and their potential antagonists, and may allow the better design of artificial growing media to improve the culturing of the olive microbiome.

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Metabolomics approach for understanding geographical dependence of soybean leaf metabolome

Cited by 31 publications

References 41 publications

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

Metabolomics as a Tool to Study Underused Soy Parts: In Search of Bioactive Compounds

High-Resolution Magic Angle Spinning (HR-MAS) NMR-Based Fingerprints Determination in the Medicinal Plant Berberis laurina

Metabolomic, Ionomic and Microbial Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype

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