Metabolomics Applications in Children: A Right Way to Go

Martire, Baldassarre; Laforgia, Nicola

doi:10.3390/metabo10090364

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…The age of the children plays a primary role in comparative metabolomics of Rb patients, as the metabolic profile of serum is dependent on the dietary effects and physical activities that vary greatly between the children of age groups 1–3 years and above 3 years. − A comparative analysis between CL3 (controls less than 3 years) and CG3 (controls greater than 3 years) was carried out to discern the metabolic differences among the controls owing to their differential age groups. PLS-DA showed distinct clusters for CL3 and CG3 with cross-validation values including accuracy = 0.86, R 2 value = 0.64, and Q 2 value = 0.35 (Figure S4).…”

Section: Resultsmentioning

confidence: 99%

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Gulati,

Manukonda,

Kairamkonda

et al. 2023

ACS Omega

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Retinoblastoma (Rb) is the most common pediatric eye cancer. To identify the biomarkers for early diagnosis and monitoring the progression of Rb in patients, mapping of the alterations in their metabolic profiles is essential. The present study aims at exploring the metabolic disparity in serum from Rb patients and controls using NMR-based metabolomics. A total of 72 metabolites, including carbohydrates, amino acids, and organic acids, were quantified in serum samples from 24 Rb patients and 26 controls. Distinct clusters of Rb patients and controls were obtained using the partial least-squares discriminant analysis (PLS-DA) model. Further, univariate and multivariate analyses of unilateral and bilateral Rb patients with respect to their age-matched controls depicted their distinct metabolic fingerprints. Metabolites including 2-phosphoglycerate, 4-aminobutyrate, proline, O -phosphocholine, O -phosphoethanolamine, and Sn-glycero-3-phosphocholine (Sn-GPC) showed significant perturbation in both unilateral and bilateral Rb patients. However, metabolic differences among the bilateral Rb cases were more pronounced than those in unilateral Rb cases with respect to controls. In addition to major discriminatory metabolites for Rb, unilateral and bilateral Rb cases showed specific metabolic changes, which might be the result of their differential genetic/somatic mutational backgrounds. This further suggests that the aberrant metabolic perturbation in bilateral patients signifies the severity of the disease in Rb patients. The present study demonstrated that identified serum metabolites have potential to serve as a noninvasive method for detection of Rb, discriminate bilateral from unilateral Rb patients, and aid in better understanding of the RB tumor biology.

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Section: Resultsmentioning

confidence: 99%

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Gulati,

Manukonda,

Kairamkonda

et al. 2023

ACS Omega

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“…Metabolomics is a systems biology approach that can be used to explore genetic and environmental influences on children’s health and disease (Baldassarre & Laforgia, 2020; Moco et al, 2013). Metabolic profiles have been assisting with characterizing markers of environmental exposures (Athersuch & Keun, 2015) and understanding health care outcomes from pre-term birth (Wilson et al, 2014) to adulthood (James et al, 2004).…”

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confidence: 99%

Metabolic Pathways Associated With Psychoneurological Symptoms in Children With Cancer Receiving Chemotherapy

Bai

Withycombe

Eldridge

2022

Biological Research For Nursing

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Context Children with cancer undergoing chemotherapy experience a cluster of psychoneurological symptoms (PNS), including pain, fatigue, anxiety, and depressive symptoms. Metabolomics is promising to differentiate metabolic pathways associated with the PNS cluster. Objectives Identify metabolic pathways associated with the PNS cluster in children with cancer before and after chemotherapy. Methods Pain, fatigue, anxiety, and depressive symptoms were assessed using the Pediatric PROMIS scales. T-scores were computed and divided dichotomously by a cutoff point of 50; the PNS cluster was a sum of the four symptoms ranging from 0 (all T-scores <50) to 4 (all T-scores ≥50). Serum metabolites were processed using liquid chromatography mass-spectrometry untargeted metabolomics approach. Linear regression models examined metabolites associated with the PNS cluster. Metabolic pathway enrichment analysis was performed. Results Participant demographics ( n = 40) were 55% female, 60% white, 62.5% aged 13–19 years, and 62.5% diagnoses of Hodgkin’s lymphoma and B-cell acute lymphocytic leukemia. Among 9276 unique metabolic features, 454 were associated with pain, 281 with fatigue, 596 with anxiety, 551 with depressive symptoms, and 300 with the PNS cluster across one chemotherapy cycle. Fatty acids pathways were associated with pain: de novo fatty acid biosynthesis ( p < .001), fatty acid metabolism ( p = .001), fatty acid activation ( p = .004), and omega-3 fatty acid metabolism ( p = .009). Tryptophan amino acid pathway was associated with fatigue ( p < .001), anxiety ( p = .015), and the PNS cluster ( p = .037). Carnitine shuttle was associated with the PNS cluster ( p = .015). Conclusion Fatty acids and amino acids pathways were associated with PNS in children undergoing chemotherapy. These findings require further investigation in a larger sample.

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Metabolomics Applications in Children: A Right Way to Go

Cited by 2 publications

References 12 publications

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Metabolic Pathways Associated With Psychoneurological Symptoms in Children With Cancer Receiving Chemotherapy

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