Metabolomics and transcriptomics indicated the molecular targets of copper to the pig kidney

Qiao, Na; Yang, Yanyang; Liao, Jianzhao; Zhang, Hui; Yang, Fan; Ma, Feiyang; Han, Qing; Yu, Wenli; Liu, Ying; Hu, Lianmei; Pan, Jiang; Hussain, Riaz; Tang, Zhaoxin

doi:10.1016/j.ecoenv.2021.112284

Cited by 29 publications

(24 citation statements)

References 78 publications

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“…Meanwhile, metabolomics analysis showed that changed metabolites were involved in glycerolphospholipid metabolism, such as LysoPC (18:1), Citicoline, Choline, PC (14:0/P-18:0), Glycerylphosphorylethanolamine, Glycerophosphocholine, CDP-Ethanolamine. Glycerophospholipids played a critical role in cell proliferation, apoptosis, and were recently reported to be a biomarker of liver injury and inflammatory response ( Qiao et al, 2021 ). For example, LysoPCs, which generated from the hydrolysis of PCs, can induce the migration of lymphocytes and macrophages, increase the production of proinflammatory cytokines, induce oxidative stress, and promote apoptosis, which can aggregate inflammation and promote the development of diseases ( Riederer et al, 2011 ; Liu et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Hepatic transcriptomics and metabolomics indicated pathways associated with immune stress of broilers induced by lipopolysaccharide

Shao

et al. 2022

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Hepatic transcriptomics and metabolomics indicated pathways associated with immune stress of broilers induced by lipopolysaccharide

Shao

et al. 2022

Poultry Science

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“…It is likely that there was progressive renal tubular damage [ 48 ]. Other studies [ 41 , 49 ] have also observed a significant increase in urea concentrations after copper exposure in a rat and pig model. The authors concluded that the bio-element can lead to renal cell death and pathological damage.…”

Section: Discussionmentioning

confidence: 76%

“…Cellular copper overload can result in cytotoxicity. Qiao et al [ 41 ] confirmed that during copper ion-induced apoptosis, there is an increase in mitochondrial membrane permeability. Arnal et al [ 42 ] found that copper (depending on concentration and cell type) can trigger cell death by activation of specific protease systems.…”

Section: Discussionmentioning

confidence: 99%

Effect of Copper on the Function of Isolated Porcine Kidneys Stored Using Simple Hypothermia

Ostróżka-Cieślik

Dolińska

Ryszka

2022

IJMS

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Renal ischemia in the peri-transplant period causes a number of changes that adversely affect the initiation of normal vital functions in grafts after transplantation. To minimise the extent of ischemic damage, organs are stored in preservation fluid. The components of the fluid are supposed to ensure stabilisation of the cell cytoskeleton, protect against oxygen free radicals, reduce cell swelling, and ensure endothelial cell integrity. The aim of this study was to analyse the protective effect of Cu2+, as a component of Biolasol preservative fluid, in the prevention of nephron damage occurring during the graft storage period. Analyses of the effectiveness of copper in the presence of prolactin added to Biolasol fluid were also carried out. Forty isolated pig slaughter kidneys were used in the study, avoiding the use of laboratory animals. The kidneys were stored using simple hypothermia. After 2 h and 48 h of graft storage, selected biochemical indicators of renal function were determined in the collected perfusates. The addition of Cu2+ at a dose of 1 µg/L to the composition of Biolasol fluid was found to affect the generation of ischemic damage in the isolated pig kidney. The intensity of the occurrence of these processes is exacerbated by the presence of prolactin at a dose of 0.1 µg/L.

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“…The present study is one of very few studies that investigated the effects of nutritional intervention on the complex metabolome of the intestinal epithelium. Numerous previous studies explored the effects of nutritional interventions on the metabolome in blood serum or plasma 34 – 37 , urine 38 , milk 39 , digesta 40 or metabolic target organs like liver 41 , 42 , kidney 43 and muscle 38 . However, only few studies have been published using porcine intestinal tissue 44 .…”

Section: Discussionmentioning

confidence: 99%

Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs

Schermuly

Romanet

Klünemann

et al. 2022

Sci Rep

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Methionine (Met) as an essential amino acid has key importance in a variety of metabolic pathways. This study investigated the influence of three dietary Met supplements (0.21% L-Met, 0.21% DL-Met and 0.31% DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA)) on the metabolome and inflammatory status in the small intestine of pigs. Epithelia from duodenum, proximal jejunum, middle jejunum and ileum were subjected to metabolomics analysis and qRT-PCR of caspase 1, NLR family pyrin domain containing 3 (NLRP3), interleukins IL1β, IL8, IL18, and transforming growth factor TGFβ. Principal component analysis of the intraepithelial metabolome revealed strong clustering of samples by intestinal segment but not by dietary treatment. However, pathway enrichment analysis revealed that after L-Met supplementation polyunsaturated fatty acids (PUFA) and tocopherol metabolites were lower across small intestinal segments, whereas monohydroxy fatty acids were increased in distal small intestine. Pigs supplemented with DL-HMTBA showed a pronounced shift of secondary bile acids (BA) and sphingosine metabolites from middle jejunum to ileum. In the amino acid super pathway, only histidine metabolism tended to be altered in DL-Met-supplemented pigs. Diet did not affect the expression of inflammation-related genes. These findings suggest that dietary supplementation of young pigs with different Met sources selectively alters lipid metabolism without consequences for inflammatory status.

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Metabolomics and transcriptomics indicated the molecular targets of copper to the pig kidney

Cited by 29 publications

References 78 publications

Hepatic transcriptomics and metabolomics indicated pathways associated with immune stress of broilers induced by lipopolysaccharide

Hepatic transcriptomics and metabolomics indicated pathways associated with immune stress of broilers induced by lipopolysaccharide

Effect of Copper on the Function of Isolated Porcine Kidneys Stored Using Simple Hypothermia

Dietary methionine source alters the lipidome in the small intestinal epithelium of pigs

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