Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria

Zhou, Cong; Song, Hao; Feng, Jie; Hu, Zhi; Yang, Mei‐Jie; Pu, Shi; Li, Yongren; Guo, Yongjun; Li, Haizhou; Zhang, Tao

doi:10.1016/j.csbj.2022.08.004

Cited by 20 publications

(5 citation statements)

References 74 publications

(84 reference statements)

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“…These findings indicated that the oxidation process of fatty acids in gills was enhanced to meet energy demand under Cd stress. This phenomenon has also been demonstrated in hypo-salinity stressed hard clams (Mercenaria mercenaria) [37].…”

Section: Exposure Caused Energy Metabolism Disorder In Gillsmentioning

confidence: 54%

“…In general, the energy supply is sufficient for the organism's needs, with excess energy being stored. Nevertheless, heavy metal stress can disrupt the energy balance of marine creatures, as the organism requires more energy to initiate stress responses, detoxification, and damage repair mechanisms [37].…”

Section: Exposure Caused Energy Metabolism Disorder In Gillsmentioning

confidence: 99%

“…Fatty acids exhibit higher energy storage efficiency compared to carbohydrates like glucose [37]. Carnitine plays a critical role in energy metabolism by facilitating the transport of long-chain fatty acids from cytoplasm to mitochondrial matrix and promoting β-oxidation of fatty acids [41].…”

Section: Exposure Caused Energy Metabolism Disorder In Gillsmentioning

confidence: 99%

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Mechanisms of Gills Response to Cadmium Exposure in Greenfin Horse-Faced Filefish (Thamnaconus septentrionalis): Oxidative Stress, Immune Response, and Energy Metabolism

Zhang,

Zhao

et al. 2024

Animals

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Cadmium (Cd) pollution has become a global issue due to industrial and agricultural developments. However, the molecular mechanism of Cd-induced detrimental effects and relevant signal transduction/metabolic networks are largely unknown in marine fishes. Here, greenfin horse-faced filefish (Thamnaconus septentrionalis) were exposed to 5.0 mg/L Cd up to 7 days. We applied both biochemical methods and multi-omics techniques to investigate how the gills respond to Cd exposure. Our findings revealed that Cd exposure caused the formation of reactive oxygen species (ROS), which in turn activated the MAPK and apoptotic pathways to alleviate oxidative stress and cell damage. Glycolysis, protein degradation, as well as fatty acid metabolism might assist to meet the requirements of nutrition and energy under Cd stress. We also found that long-term (7 days, “long-term” means compared to 12 and 48 h) Cd exposure caused the accumulation of succinate, which would in turn trigger an inflammatory response and start an immunological process. Moreover, ferroptosis might induce inflammation. Overall, Cd exposure caused oxidative stress, energy metabolism disturbance, and immune response in greenfin horse-faced filefish. Our conclusions can be used as references for safety risk assessment of Cd to marine economic fishes.

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Section: Exposure Caused Energy Metabolism Disorder In Gillsmentioning

confidence: 54%

Section: Exposure Caused Energy Metabolism Disorder In Gillsmentioning

confidence: 99%

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Mechanisms of Gills Response to Cadmium Exposure in Greenfin Horse-Faced Filefish (Thamnaconus septentrionalis): Oxidative Stress, Immune Response, and Energy Metabolism

Zhang,

Zhao

et al. 2024

Animals

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“…Modulations of proline, proline betaine, betaine, and trigonelline in Zn-anode-exposed oysters are also related to osmoregulation [ 43 , 44 , 45 ]. These molecules affect the ionic strength of the cytosol and thereby maintain osmotic pressure within the cell.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics-Based Investigation on the Metabolic Changes in Crassostrea gigas Experimentally Exposed to Galvanic Anodes

et al. 2023

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Cathodic protection is widely used to protect metal structures from corrosion in marine environments using sacrificial galvanic anodes. These anodes, either in Zinc, or preferentially nowadays in Al-Zn-In alloys, are expected to corrode instead of the metal structures. This leads to the release of dissolved species, Zn2+, Al3+, and In3+, and solid phases such as Al(OH)3. Few studies have been conducted on their effects on marine organisms, and they concluded that further investigations are needed. We therefore evaluated the effects of Zn and Al-Zn-In anodes on oysters stabulated in tanks, under controlled conditions defined through a comparison with those prevailing in a given commercial seaport used as reference. We analyzed the entire metabolome of gills with a non-targeted metabolomic approach HRMS. A modelling study of the chemical species, corresponding to the degradation products of the anodes, likely to be present near the exposed oysters, was also included. We identified 16 and two metabolites modulated by Zn- and Al-Zn-In-anodes, respectively, that were involved in energy metabolism, osmoregulation, oxidative stress, lipid, nucleotide nucleoside and amino acid metabolisms, defense and signaling pathways. The combination of chemical modelling and metabolomic approach, used here for the first time, enlightened the influence of Zn present in the Al-Zn-In anodes.

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“…Global climate change has led to a reduction in seawater salinity, such as extreme rainfall, and this reduction may affect the physiological functions and survival of marine bivalves [25][26][27][28]. Hyposalinity can induce osmotic stress in bivalves, potentially leading to mortality in extreme cases [29][30][31][32][33][34].…”

Section: Salinitymentioning

confidence: 99%

Effects of Nutrient Source, Temperature, and Salinity on the Growth and Survival of Three Giant Clam Species (Tridacnidae)

Lee,

Lin,

Huang

et al. 2024

Animals

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The habitats of giant clams are undergoing environmental changes, and giant clam populations are declining. The present study was conducted to facilitate clam conservation. We conducted three 18-week trials to investigate the effects of nutrient, temperature, and salinity on the growth performance and survival rates (SRs) of juvenile Tridacna noae, adult Tridacna crocea, and subadult Tridacna derasa, respectively. Regarding nutrient sources, no significant differences were observed in shell length gain, specific growth rate, or SR between clams fed with Chaetoceros muelleri or commercial feed (hw nanotip) and those in a control group (juvenile phototrophs). Regarding temperature, clams cultivated at 27 °C exhibited significantly better growth performance and SR than did those cultivated at 19 °C or 31 °C (p < 0.05). By week 6, all clams in the 19 °C and 31 °C groups had died, indicating that suboptimal growth temperatures have severe adverse effects. Regarding salinity, clams cultivated at 34‰ exhibited significantly higher length gains and specific growth rates than did those cultivated at 20‰ or 25‰ (p < 0.05). SR was not significantly affected by salinity. Understanding how environmental factors affect giant clam populations may help researchers devise effective clam conservation strategies.

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Metabolomics and biochemical assays reveal the metabolic responses to hypo-salinity stress and osmoregulatory role of cAMP-PKA pathway in Mercenaria mercenaria

Cited by 20 publications

References 74 publications

Mechanisms of Gills Response to Cadmium Exposure in Greenfin Horse-Faced Filefish (Thamnaconus septentrionalis): Oxidative Stress, Immune Response, and Energy Metabolism

Mechanisms of Gills Response to Cadmium Exposure in Greenfin Horse-Faced Filefish (Thamnaconus septentrionalis): Oxidative Stress, Immune Response, and Energy Metabolism

Metabolomics-Based Investigation on the Metabolic Changes in Crassostrea gigas Experimentally Exposed to Galvanic Anodes

Effects of Nutrient Source, Temperature, and Salinity on the Growth and Survival of Three Giant Clam Species (Tridacnidae)

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