Metabolomics analysis to evaluate the antibacterial activity of the essential oil from the leaves of Cinnamomum camphora (Linn.) Presl

Chen, Jiali; Tang, Cailin; Zhang, Rongfei; Ye, Shaoxia; Zhao, Zhimin; Huang, Yuquan; Xu, Xin; Lan, Wen‐Jian; Yang, Dan

doi:10.1016/j.jep.2020.112652

Cited by 97 publications

(69 citation statements)

References 38 publications

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“…Then, the supernatants were concentrated in a rotary vacuum centrifuge device (LABCONCO). The dried extracts were used for GC-MS analysis, as described in a previous study [ 12 ]. In brief, samples were derivatized.…”

Section: Methodsmentioning

confidence: 99%

“…A growing number of pharmacological researches have shown that C. camphora exhibits a wide range of biological activities such as anti-inflammation, anti-bacteria, antioxidation, anti-fungi, and repellent properties [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. In our previous study [ 12 ], 48 constituents from EO were identified; linalool was the major compound, followed by eucalyptol, isoborneol, α-terpineol, and camphor ( Supplementary Material, Table S1 ).…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

et al. 2020

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Cinnamomum camphora (Linn.) Presl has been widely used in traditional Chinese medicine for a variety of purposes. Our previous study indicated the antibacterial mechanism of the essential oil (EO) from C. camphora leaves; however, its anti-inflammatory activity and the underlying mechanism have not been clearly demonstrated. Thus, the present study investigated its anti-inflammatory property. Our data revealed that EO significantly decreased the release of nitric oxide (NO) and the mRNA expression of inducible NO synthase (iNOS) in lipopolysaccharide (LPS)-induced BV2 microglial cells. EO also attenuated LPS-induced increase in the mRNA expression and secretion of inflammatory cytokines including interleukin-6 (IL-6), IL-18, IL-1β and tumor necrosis factor-α (TNF-α). Furthermore, the metabolic profiles of LPS-induced BV2 microglial cells treated with or without EO were explored. Thirty-nine metabolites were identified with significantly different contents, including 21 upregulated and 18 downregulated ones. Five pathways were enriched by shared differential metabolites. Compared with the control cells, the glucose level was decreased, while the lactate level was increased, in the culture supernatant from LPS-stimulated cells, which were reversed by EO treatment. Moreover, compared to the LPS-treated group, the activities of phosphofructokinase (PFK) and pyruvate kinase (PK) in EO group were decreased. In summary, the current study demonstrated that EO from C. camphora leaves acts as an anti-inflammatory agent, which might be mediated through attenuating the glycolysis capacity of microglial cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

et al. 2020

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“…A P value less than 0.05 was considered as a statistically signi cant difference. Metabolomics data was analyzed based on the technique described in our previous study 12 .…”

Section: Discussionmentioning

confidence: 99%

“…Then the supernatants were concentrated in a rotary vacuum centrifuge device (LABCONCO). The dried extracts were used for GC-MS analysis, as described in previous study 12 . Each sample contained six biological and two technical replicates.…”

Section: Primermentioning

confidence: 99%

“…A growing number of pharmacological researches have shown that C. camphora exhibits a wide range of biological activities such as anti-in ammation, antibacteria, antioxidation, anti-fungi, and repellent properties [3][4][5][6][7][8][9][10][11] . In our previous study 12 , 48 constituents from EO were identi ed; and linalool was the major compound (26.6%), followed by eucalyptol (16.8%), αterpineol (8.7%), isoborneol (8.1%), β-phellandrene (5.1%), and camphor (5.0%).…”

Section: Introductionmentioning

confidence: 97%

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Anti-inflammatory Property of the Essential Oil From Cinnamomum Camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

Chen

Tang

Zhou

et al. 2020

Preprint

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Background: Cinnamomum camphora (Linn.) Presl has been widely used in traditional Chinese medicine for a variety of purposes. Our previous study indicated the antibacterial mechanism of the essential oil (EO) from C. camphora; however, the anti-inflammatory activity of EO and its underlying mechanism have not been clearly demonstrated. The present study aims to evaluate the anti-inflammatory principle and mechanism of EO.Methods: The anti-inflammatory activity of EO was evaluated in lipopolysaccharide (LPS)-induced BV2 microglial cells. Nitric oxide (NO) production was measured by NO assay kit. The mRNA expression levels of inducible NO synthase (iNOS), interleukin-6 (IL-6), IL-18 and IL-1β were examined by real time-PCR (RT-PCR). The secretion of pro-inflammatory cytokines in cell supernatants, including IL-6, IL-18 and IL-1β, were assessed by ELISA kits. Furthermore, the metabolic profile of BV2 microglial cells treated with or without EO was explored by GC-MS-based metabolomics analysis. Phosphofructokinase (PFK) and pyruvate kinase (PK) activities were detected by commercial kits.Results: EO significantly decreased the release of NO and the mRNA expression of iNOS in LPS-induced BV2 microglial cells. EO also attenuated LPS-induced increase in the mRNA expression and secretion of inflammatory cytokines including IL-6, IL-18 and IL-1β. 39 metabolites were identified with significantly different contents, including 21 upregulated and 18 downregulated ones, in the metabolomics analysis. Five pathways were enriched by shared differential metabolites. Additionally, compared with the control group, the glucose level was decreased, while the lactate level was increased, in the culture supernatant of LPS-induced BV2 microglia cells, which were reversed by EO treatment. Besides, compared to the LPS-treated group, the activities for PK and PFK in EO group were decreased by 17.59% and 18.23%, respectively.Conclusions: The EO from C. camphora acts as an anti-inflammatory agent, which might be mediated through attenuating the glycolysis capacity of microglial cells.

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Antifungal and mycotoxin detoxification ability of essential oils: A review

Cai

Yan

Shi

et al. 2021

Phytotherapy Research

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With increased popular awareness of food safety and environmental protection, plant essential oil has attracted interest due to the absence of residue, its high efficiency, antioxidant, immune regulation, antibacterial, insecticidal, and other advantages. Their application in degradation and elimination of mycotoxin toxicity has attracted increasing attention. This paper reviews the structure, antibacterial activity, antibacterial mechanism, and toxic effects of essential oils. The inhibitory effects of various essential oils on different mycotoxins were studied. The research progress on the inhibitory effects of plant essential oils on fungi and mycotoxins in recent years was summarized to provide reference for the application of plant essential oils.

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Metabolomics analysis to evaluate the antibacterial activity of the essential oil from the leaves of Cinnamomum camphora (Linn.) Presl

Cited by 97 publications

References 38 publications

Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

Anti-inflammatory Property of the Essential Oil From Cinnamomum Camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis

Antifungal and mycotoxin detoxification ability of essential oils: A review

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