Metabolomics Analysis Reveals Major Differential Metabolites and Metabolic Alterations in Tea Plant Leaves (Camellia sinensis L.) Under Different Fluorine Conditions

Luo, Jinlei; Hu, Kai; Qu, Fengfeng; Ni, Dejiang; Zhang, Haojie; Liu, Siyi; Chen, Yuqiong

doi:10.1007/s00344-020-10141-0

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…As shown in Table S2 , nine organic acids were all upregulated in two G. lucidum , and only the content of C19:1 (CIS-10) acid was downregulated, compared to that of the inoculation. The reference showed that organic acids are closely related to the synthesis and catabolism of the cell wall [ 15 , 16 ]. The cell wall is the first barrier for external ions entering the protoplasm, which plays an important role in the resistance to external ion stress [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

et al. 2022

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Ganoderma lucidum has been used as a rare medical mushroom for centuries in China, due to its health-promoting properties. Successive cropping obstacles are common in the cultivation of G. lucidum, although the remaining nutrients in the germ substrate are sufficient for a second fruiting. Here, we aimed to study the metabolite profile of G. lucidum via nontargeted metabonomic technology. Metabonomic data revealed that organic acids played an important role in the cropping obstacles of G. lucidum, which is accordance with the pH decrease in the germ substrate. A Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis indicated that most differential acids participated in the metabolic pathways. Five acids were all significantly upregulated by two MS with high energy (MSE) modes in two cultivars, among which 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylic acid is also involved in purine metabolism regulation and microbial metabolism in diverse environments. Taken together, this work illustrated the organic acid stress generated by G. lucidum, which formed the autotoxicity feedback, and resulted in cropping obstacles. Determining the cause of the cropping obstacles in G. lucidum will promote the utilization rate of fungus substrate to realize the sustainable use of this resource.

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Section: Resultsmentioning

confidence: 99%

Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

et al. 2022

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“…In tea plant response to other abiotic stresses (i.e. ionic stresses), the pectin biosynthesis was shown to be promoted under the regulation of pectin genes like GAUT [ 24 , 25 ]. However, there has been no report available on the change of pectin level in tea plant under low temperature stress.…”

Section: Discussionmentioning

confidence: 99%

“…To verify the accuracy of RNA-seq data, the relative gene expression of twenty key DEGs involved in cell wall metabolism was detected by qRT-PCR. The experiment was carried out as described in our previous study with the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as an internal control [ 25 ], and the primers are listed in Supplemental Table S3.…”

Section: Methodsmentioning

confidence: 99%

Physiological and transcriptomic analyses reveal tea plant (Camellia sinensis L.) adapts to extreme freezing stress during winter by regulating cell wall structure

Luo,

Huang,

Chang

et al. 2023

BMC Genomics

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Tea plants grown in high-latitude areas are often damaged by extreme freezing temperatures in winter, leading to huge economic losses. Here, the physiological and gene expression characteristics of two tea cultivars (Xinyang No. 10 (XY10), a freezing-tolerant cultivar and Fudingdabaicha (FDDB), a freezing-sensitive cultivar) during overwintering in northern China were studied to better understand the regulation mechanisms of tea plants in response to natural freezing stress. Samples were collected at a chill (D1), freezing (D2) and recovery (D3) temperature in winter. TEM analysis of integrated leaf ultrastructure at D2 revealed lower malondialdehyde and relative electrical conductivity in XY10 than in FDDB, with serious cell structure damage in the latter, indicating XY10 was more resistant to freezing stress. Differential gene expression analysis among the different samples over winter time highlighted the following gene functions in cell wall metabolism (CesAs, COBLs, XTHs, PGs, PMEs), transcription factors (ERF1B and MYC2), and signal transduction (CDPKs and CMLs). The expression pattern of cellulose and pectin-related genes suggested higher accumulation of cellulosic and pectic materials in the cell wall of XY10, agreeing with the results of cell wall and its components. These results indicated that under the regulation of cell wall genes, the freezing-resistant tea cultivar can better maintain a well-knit cell wall structure with sufficient substances to survive natural freezing damage. This study demonstrated the crucial role of cell wall in tea plant resistance to natural freezing stress and provided important candidate genes for breeding of freezing-resistant tea cultivars.

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“…The variable weight for the project (VIP) and fold change obtained by OPLA -DA model were combined to analyze and screen differential metabolites. VIP >1, p -value <0.05 and fold change >2 were used as criteria to screen the significantly changed metabolites ( Luo et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

Ling

Wang²,

Pan³

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Plant cell culture technology is a potential way to produce polyphenols, however, this way is still trapped in the dilemma of low content and yield. Elicitation is regarded as one of the most effective ways to improve the output of the secondary metabolites, and therefore has attracted extensive attention.Methods: Five elicitors including 5-aminolevulinic acid (5-ALA), salicylic acid (SA), methyl jasmonate (MeJA), sodium nitroprusside (SNP) and Rhizopus Oryzae Elicitor (ROE) were used to improve the content and yield of polyphenols in the cultured Cyclocarya paliurus (C. paliurus) cells, and a co-induction technology of 5-ALA and SA was developed as a result. Meanwhile, the integrated analysis of transcriptome and metabolome was adopted to interpret the stimulation mechanism of co-induction with 5-ALA and SA.Results: Under the co-induction of 50 μM 5-ALA and SA, the content and yield of total polyphenols of the cultured cells reached 8.0 mg/g and 147.12 mg/L, respectively. The yields of cyanidin-3-O-galactoside, procyanidin B1 and catechin reached 28.83, 4.33 and 2.88 times that of the control group, respectively. It was found that expressions of TFs such as CpERF105, CpMYB10 and CpWRKY28 increased significantly, while CpMYB44 and CpTGA2 decreased. These great changes might further make the expression of CpF3′H (flavonoid 3′-monooxygenase), CpFLS (flavonol synthase), CpLAR (leucoanthocyanidin reductase), CpANS (anthocyanidin synthase) and Cp4CL (4-coumarate coenzyme A ligase) increase while CpANR (anthocyanidin reductase) and CpF3′5′H (flavonoid 3′, 5′-hydroxylase) reduce, ultimately enhancing the polyphenols accumulationConclusion: The co-induction of 5-ALA and SA can significantly promote polyphenol biosynthesis in the cultured C. paliurus cells by regulating the expression of key transcription factors and structural genes associated with polyphenol synthesis, and thus has a promising application.

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Metabolomics Analysis Reveals Major Differential Metabolites and Metabolic Alterations in Tea Plant Leaves (Camellia sinensis L.) Under Different Fluorine Conditions

Cited by 8 publications

References 43 publications

Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

Autotoxicity of Endogenous Organic Acid Stress in Two Ganoderma lucidum Cultivars

Physiological and transcriptomic analyses reveal tea plant (Camellia sinensis L.) adapts to extreme freezing stress during winter by regulating cell wall structure

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

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