Metabolomics Analysis of the Larval Head of the Silkworm, Bombyx mori

Li, Yi; Wang, Xin; Chen, Quanmei; Hou, Yong; Xia, Qingyou; Zhao, Ping

doi:10.3390/ijms17091460

Cited by 21 publications

(12 citation statements)

References 33 publications

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“…Our identification of fatty acid amides in Bmi4 is the first report of these lipid amides in B. mori . Long-chain fatty acids are known in B. mori [53–56] and the only report of a related N -acylamide in B. mori is N -acetylglutamate [56]. Fatty acid amides have a long and underappreciated history in insects, starting with the discovery of volicitin, N -(17-hydroxylinolenoyl)-L-glutamine, from Spodoptera exigua [15].…”

Section: Discussionmentioning

confidence: 99%

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

Anderson

Battistini

Wallis

et al. 2018

Prostaglandins, Leukotrienes and Essential Fatty Acids

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The purpose of this research is to unravel the substrate specificity and kinetic properties of an insect arylalkylamine N-acyltransferase from Bombyx mori (Bm-iAANAT) and to determine if this enzyme will catalyze the formation of long chain N-acylarylalkylamides in vitro. However, the determination of substrates and products for Bm-iAANAT in vitro is no guarantee that these same molecules are substrates and products for the enzyme in the organism. Therefore, RT-PCR was performed to detect the Bm-iAANAT transcripts and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis was performed on purified lipid extracts from B. mori larvae (fourth instar, Bmi4) to determine if long chain fatty acid amides are produced in B. mori. Ultimately, we found that recombinant Bm-iAANAT will utilize long-chain acyl-CoA thioesters as substrates and identified Bm-iAANAT transcripts and long-chain fatty acid amides in Bmi4. Together, these data show Bm-iAANAT will catalyze the formation of long-chain N-acylarylalkylamides in vitro and provide evidence demonstrating that Bm-iAANAT has a role in fatty acid amide biosynthesis in B. mori, as well.

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Section: Discussionmentioning

confidence: 99%

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

Anderson

Battistini

Wallis

et al. 2018

Prostaglandins, Leukotrienes and Essential Fatty Acids

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“…To reveal the metabolic differences of BmE cells infected with BmNPV at 0, 3, 6, 12, 24, 48, and 72 hpi, the metabolites identified were subjected to differential analysis. Differential metabolites were identified following a previously described method [ 46 ], with only metabolites that changed ≥2 fold (enriched metabolites) or ≤0.5 (decreased metabolites) in relative ratios ( p -value < 0.05) considered to be significantly altered.…”

Section: Methodsmentioning

confidence: 99%

The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori

Hua

Zhang

Xü

et al. 2021

IJMS

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Bombyx mori nucleopolyhedrovirus (BmNPV) is a pathogen that causes great economic losses in sericulture. Many genes play a role in viral infection of silkworms, but silkworm metabolism in response to BmNPV infection is unknown. We studied BmE cells infected with BmNPV. We performed liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based non-targeted metabolomics analysis of the cytosolic extract and identified 36, 76, 138, 101, 189, and 166 different molecules at 3, 6, 12, 24, 48, and 72 h post BmNPV infection (hpi) compared with 0 hpi. Compounds representing different areas of metabolism were increased in cells post BmNPV infection. These areas included purine metabolism, aminoacyl−tRNA biosynthesis, and ABC transporters. Glycerophosphocholine (GPC), 2-hydroxyadenine (2-OH-Ade), gamma-glutamylcysteine (γ-Glu-Cys), hydroxytolbutamide, and 5-pyridoxolactone glycerophosphocholine were continuously upregulated in BmE cells post BmNPV infection by heat map analysis. Only 5-pyridoxolactone was found to strongly inhibit the proliferation of BmNPV when it was used to treat BmE cells. Fewer infected cells were detected and the level of BmNPV DNA decreased with increasing 5-pyridoxolactone in a dose-dependent manner. The expression of BmNPV genes ie1, helicase, GP64, and VP39 in BmE cells treated with 5-pyridoxolactone were strongly inhibited in the BmNPV infection stage. This suggested that 5-pyridoxolactone may suppress the entry of BmNPV. The data in this study characterize the metabolism changes in BmNPV-infected cells. Further analysis of 5-pyridoxolactone, which is a robust antiviral molecule, may increase our understanding of antiviral immunity.

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“…From the execution of the previously defined search string [34] in Scopus, 326 papers were obtained, of which the title, abstract and keywords were evaluated. From those, 107 were rejected according to the established inclusion and exclusion criteria, that is, all works that were not related to food-intake (for example, References [37,38]), or regarding food-intake in animals were excluded (for example References [39,40]).…”

Section: Paper Classificationmentioning

confidence: 99%

Systematic Literature Review of Food-Intake Monitoring in an Aging Population

Moguel

Berrocal

García-Alonso

2019

Sensors

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The dietary habits of people directly impact their health conditions. Especially in elder populations (in 2017, 6.7% of the world’s population was over 65 years of age), these habits could lead to important-nutrient losses that could seriously affect their cognitive and functional state. Recently, a great research effort has been devoted to using different technologies and proposing different techniques for monitoring food-intake. Nevertheless, these techniques are usually generic but make use of the most innovative technologies and methodologies to obtain the best possible monitoring results. However, a large percentage of elderly people live in depopulated rural areas (in Spain, 28.1% of the elderly population lives in this type of area) with a fragile cultural and socioeconomic context. The use of these techniques in these environments is crucial to improving this group’s quality of life (and even reducing their healthcare expenses). At the same time, it is especially challenging since they have very specific and strict requirements regarding the use and application of technology. In this Systematic Literature Review (SLR), we analyze the most important proposed technologies and techniques in order to identify whether they can be applied in this context and if they can be used to improve the quality of life of this fragile collective. In this SLR, we have analyzed 326 papers. From those, 29 proposals have been completely analyzed, taking into account the characteristics and requirements of this population.

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Metabolomics Analysis of the Larval Head of the Silkworm, Bombyx mori

Cited by 21 publications

References 33 publications

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

The Antiviral Molecule 5-Pyridoxolactone Identified Post BmNPV Infection of the Silkworm, Bombyx mori

Systematic Literature Review of Food-Intake Monitoring in an Aging Population

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