Metabolomics analysis for skin ulceration syndrome of Apostichopus japonicus based on UPLC/Q-TOF MS

Zhang, Yang; Wang, Youquan; Liu, Xiaoyu; Ding, Beichen; Sun, Yuena; Chang, Yaqing; Ding, Jun

doi:10.1007/s00343-020-0205-4

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…LC-MS analysis revealed that downregulated metabolites were associated with fatty acid metabolism, carbohydrate metabolism, and the TCA cycle. UPLC-QTOF MS was used to describe the metabolic changes induced by skin ulceration syndrome, the main disease affecting the development of Apostichopus japonicus in the aquaculture industry [ 175 ]. As a result, variations in metabolites mainly related to amino acid metabolism, energy metabolism, immunity, osmoregulation, and neuroactive ligand-receptor interaction has been discovered.…”

Section: Applications Of Metabolome-oriented Approaches In Studies Of...mentioning

confidence: 99%

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

2022

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Today, marine natural products are considered one of the main sources of compounds for drug development. Starfish and sea cucumbers are potential sources of natural products of pharmaceutical interest. Among their metabolites, polar steroids, triterpene glycosides, and polar lipids have attracted a great deal of attention; however, studying these compounds by conventional methods is challenging. The application of modern MS-based approaches can help to obtain valuable information about such compounds. This review provides an up-to-date overview of MS-based applications for starfish and sea cucumber bioactive compounds analysis. While describing most characteristic features of MS-based approaches in the context of starfish and sea cucumber metabolites, including sample preparation and MS analysis steps, the present paper mainly focuses on the application of MS-based metabolic profiling of polar steroid compounds, triterpene glycosides, and lipids. The application of MS in metabolomics studies is also outlined.

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Section: Applications Of Metabolome-oriented Approaches In Studies Of...mentioning

confidence: 99%

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

2022

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“…Interspecies variation and geographic discrimination were demonstrated through metabolite fingerprinting and multivariate statistics [5,15]. Pathway analysis also revealed differentially expressed metabolites during the evisceration process [16]; in the presence of thermal stress and hypoxia [17]; and skin ulceration syndrome [18]. Literature on specific biological aspects of A. japonicus, however, did not include comprehensive characterization of small molecules outside statistically significant features corresponding to primary metabolites (amino acids and derivatives) and a number of lipids [5,[15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive metabolomics of Philippine Stichopus cf. horrens reveals diverse classes of valuable small molecules for biomedical applications

Torreno,

Molino,

Junio

et al. 2023

PLoS ONE

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Stichopus cf. horrens is an economically important sea cucumber species in Southeast Asia due to their presumed nutritional and medicinal benefits. However, compared to other sea cucumbers such as Apostichopus japonicus, there are no biochemical studies on which compounds contribute to the purported bioactivities of S. cf. horrens. To address this, a high-throughput characterization of the global metabolite profile of the species was performed through LC-MS/MS experiments and utilizing open-access platforms such as GNPS, XCMS, and metaboAnalyst. Bioinformatics-based molecular networking and chemometrics revealed the abundance of phospholipids such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), and phosphatidylserines (PSs) in the crude samples. Body wall extracts were observed to have higher levels of structural, diacylated PCs, while the viscera have higher relative abundance of single-tail PCs and PEs that could be involved in digestion via nutrient absorption and transport for sea cucumbers. PEs and sphingolipids could also be implicated in the ecological response and morphological transformations of S. cf. horrens in the presence of predatory and other environmental stress. Interestingly, terpenoid glycosides and saponins with reported anti-cancer benefits were significantly localized in the body wall. The sulfated alkanes and sterols present in S. cf. horrens bear similarity to known kairomones and other signaling molecules. All in all, the results provide a baseline metabolomic profile of S. cf. horrens that may further be used for comparative and exploratory studies and suggest the untapped potential of S. cf. horrens as a source of bioactive molecules.

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Metabolic perturbations and bacterial communities dysbiosis associated with skin ulcer disease progression in Sepia pharaonis

Xu,

Jiang,

Peng

et al. 2024

Aquaculture

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Metabolomics analysis for skin ulceration syndrome of Apostichopus japonicus based on UPLC/Q-TOF MS

Cited by 5 publications

References 51 publications

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

Comprehensive metabolomics of Philippine Stichopus cf. horrens reveals diverse classes of valuable small molecules for biomedical applications

Metabolic perturbations and bacterial communities dysbiosis associated with skin ulcer disease progression in Sepia pharaonis

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