Metabolomics Analyses Provide Insights Into Nutritional Value and Abiotic Stress Tolerance in Halophyte Halogeton glomeratus

Abstract

Halogeton glomeratus is a succulent annual herbaceous halophyte belonging to the Chenopodiaceae family, has attracted wide attention as a promising candidate for phytoremediation and as an oilseed crop and noodle-improver. More importantly, H. glomeratus has important medicinal value in traditional Chinese medicine. However, there are few comprehensive studies on the nutrients, particularly secondary metabolites. Here, we adopted untargeted metabolomics to compare the differences in metabolites of different tissues (root, stem, leaf, and seed) and identify the compounds related to pharmacological effects and response to abiotic stress in H. glomeratus. A total of 2,152 metabolites were identified, and the metabolic profiles of root, stem, leaf, and seed samples were clearly separated. More than 50% of the metabolites showed significant differences among root, stem, leaf, and seed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differential metabolites suggested an extensive alteration in the metabolome among the different organs. Furthermore, the identified metabolites related to pharmacological effects and response to abiotic stress included flavones, flavonols, flavandiols, glucosinolates, isoquinolines, pyridines, indoles, amino acids, lipids, carbohydrates, and ATP-binding cassette transporters. These metabolites have application in treating human cardiovascular diseases, cancers, diabetes, and heart disease, induce sleeping and have nutritive value. In plants, they are related to osmotic adjustment, alleviating cell damage, adjusting membrane lipid action and avoiding toxins. To the best of our knowledge, this is the first metabolomics-based report to overview the metabolite compounds in H. glomeratus and provide a reference for future development and utilization of H. glomeratus.

Combined Proteomic and Metabolomic Analysis of the Molecular Mechanism Underlying the Response to Salt Stress during Seed Germination in Barley

“…The quality control (QC) sample was prepared by a mixture of equal amounts of the supernatants from all of the samples. A UHPLC system (Thermo UltiMate 3000) with a UPLC HSS T3 column (2.1 mm × 150 mm, 1.8 μm) coupled to a Q Exactive system (Orbitrap MS, Thermo) was used for LC-MS/MS analyses according to the method described by Wang et al [ 113 ]. The acquired raw MS data files were converted to mzXML format using Proteowizard (v3.0.8789).…”

Exogenous Melatonin Enhances the Low Phosphorus Tolerance of Barley Roots of Different Genotypes

“…Metabolite extraction was performed according to the method described by Wang et al [48]. Briefly, approximately 80 mg of barley root was ground with liquid nitrogen and the obtained powder was homogenized in 1 mL of methanol/acetonitrile/H 2 O (2:2:1, v/v/v), vortexed and centrifuged at 14,000× g for 15 min at 4 • C. The supernatant was collected in LC-MS vials transferred for UHPLC-QE orbitrap/MS analysis.…”