Metabolomics: A Primer

Liu, Xiaojing; Locasale, Jason W.

doi:10.1016/j.tibs.2017.01.004

Cited by 283 publications

(199 citation statements)

References 117 publications

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“…The metabolome is affected by both internal and external factors/stimuli and, therefore, directly reflects the underlying biochemical activity and status of the biological system in question. Metabolic profiles may provide a wealth of information which can be used for disease prediction, disease progression, and treatment outcome .…”

Section: Introductionmentioning

confidence: 99%

CE–MS for anionic metabolic profiling: An overview of methodological developments

2019

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The efficient profiling of highly polar and charged metabolites in biological samples remains a huge analytical challenge in metabolomics. Over the last decade, new analytical techniques have been developed for the selective and sensitive analysis of polar ionogenic compounds in various matrices. Still, the analysis of such compounds, notably for acidic ionogenic metabolites, remains a challenging endeavor, even more when the available sample size becomes an issue for the total analytical workflow. In this paper, we give an overview of the possibilities of capillary electrophoresis‐mass spectrometry (CE–MS) for anionic metabolic profiling by focusing on main methodological developments. Attention is paid to the development of improved separation conditions and new interfacing designs in CE–MS for anionic metabolic profiling. A complete overview of all CE–MS‐based methods developed for this purpose is provided in table format (Table 1) which includes information on sample type, separation conditions, mass analyzer and limits of detection (LODs). Selected applications are discussed to show the utility of CE–MS for anionic metabolic profiling, especially for small‐volume biological samples. On the basis of the examination of the reported literature in this specific field, we conclude that there is still room for the design of a highly sensitive and reliable CE–MS method for anionic metabolic profiling. A rigorous validation and the availability of standard operating procedures would be highly favorable in order to make CE–MS an alternative, viable analytical technique for metabolomics.

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Section: Introductionmentioning

confidence: 99%

CE–MS for anionic metabolic profiling: An overview of methodological developments

2019

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“…Metabolomics is a field of study aiming to comprehensively analyze metabolites through the use of various analytical detection methods, namely mass spectrometry (MS) and nuclear magnetic resonance (NMR) (Liu and Locasale, 2017). Although both NMR and MS are essential tools in metabolomics, mass spectrometry can analyze samples with greater sensitivity (Liu and Locasale, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Although both NMR and MS are essential tools in metabolomics, mass spectrometry can analyze samples with greater sensitivity (Liu and Locasale, 2017). Within mass spectrometry, various approaches are available, however a rapid approach to profile the global metabolome is needed (Lv et al ., 2011; Rhoades and Weljie, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Advancements in triple quadrupole mass spectrometers have made them well suited for ion-switching methods (scanning in both positive and negative ion modes within a single analysis) in addition to enabling reproducible and sensitive targeted profiling of numerous metabolites (Lv et al ., 2011; Gika et al ., 2012; Yuan et al ., 2012; Rhoades and Weljie, 2016). Additionally, LC typically does not require extensive sample preparation, nor derivatization, which allows for the detection of a broader range of metabolites (Gika et al ., 2012; Liu and Locasale, 2017). Nonetheless, development of LC-MS methods to comprehensively analyze small polar metabolites is nontrivial, and requires advanced modeling to optimize both LC and MS factors simultaneously.…”

Section: Introductionmentioning

confidence: 99%

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Extraction and Analysis of Pan-metabolome Polar Metabolites by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)

2018

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Modern triple quadrupole mass spectrometers provide the ability to detect and quantify a large number of metabolites using tandem mass spectrometry (MS/MS). Liquid chromatography (LC) is advantageous, as it does not require derivatization procedures and a large diversity in physiochemical characteristics of analytes can be accommodated through a variety of column chemistries. Recently, the comprehensive optimization of LC-MS metabolomics using design of experiments (COLMeD) approach has been described and used by our group to develop robust LC-MS workflows (Rhoades and Weljie, 2016). The optimized LC-MS/MS method described here has been utilized extensively for metabolomics analysis of polar metabolites. Typically, tissue or biofluid samples are extracted using a modified Bligh-Dyer protocol (Bligh and Dyer, 1959; Tambellini et al., 2013). The protocol described herein describes this workflow using targeted polar metabolite multiple reaction monitoring (MRM) from tissues and biofluids via ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). This workflow has been utilized extensively for chronometabolic analysis (Krishnaiah et al., 2017), with applications generalized to other types of analyses as well (Sengupta et al., 2017; Sivanand et al., 2017).

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“…Metabolomics is a relatively new member of the –omics family and an increasingly powerful tool for understanding cellular and systemic metabolism. It is generally defined as the qualitative and quantitative analysis of small‐molecule metabolites (<1,000 Da) involved in various biochemical reactions in biological systems . It aims to characterize the ultimate status of physiology or pathophysiology by measuring the time‐dependent metabolite alterations in a global metabolic network .…”

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confidence: 99%

Emerging Applications of Metabolomics in Clinical Pharmacology

Pang

Wang

2019

Clin Pharma and Therapeutics

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Metabolic disturbances have been associated with many human diseases, including cancer, diabetes, and cardiovascular disease. Metabolomics, a rapidly growing member of the –omics family, investigates cellular metabolism by quantifying metabolites on a large scale and provides a link between metabolic pathways and the upstream genome that governs them. With the advances in analytical technologies, metabolomics is becoming a powerful tool for identifying diagnostic biomarkers of diseases, elucidating the pathological mechanisms, discovering novel drug targets, predicting drug responses, interpreting the mechanisms of drug action, as well as enabling precision treatment of patients. In this review, we highlight the recent advances of technologies and methodologies in metabolomics and their applications to the field of clinical pharmacology. Recent publications from 2013 to 2018 are covered in the review, and current challenges and potential future directions in the field are also discussed.

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Metabolomics: A Primer

Cited by 283 publications

References 117 publications

CE–MS for anionic metabolic profiling: An overview of methodological developments

CE–MS for anionic metabolic profiling: An overview of methodological developments

Extraction and Analysis of Pan-metabolome Polar Metabolites by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS)

Emerging Applications of Metabolomics in Clinical Pharmacology

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