Metabolomic study of two rice lines infected by Rhizoctonia solani in negative ion mode by CE/TOF-MS

Suharti, Woro Sri; Nose, Akihiro; Zheng, Shao-Hui

doi:10.1016/j.jplph.2016.09.004

Cited by 27 publications

(26 citation statements)

References 48 publications

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“…[19]. Moreover, the results were consistent with the defense response metabolites upregulated by Rhizoctonia solani infecting rice [9]. Table 3).…”

Section: Resultssupporting

confidence: 82%

“…The result indicated that these metabolic pathways were activated during the infection process. Suharti et al (2016) reported that carbohydrate metabolic pathway and amino acid metabolic pathway were the main pathways being regulated when resistant rice was infected with Rhizoctonia solani [9]. In this study, these two pathways were also identified at three time points after analyzing differential metabolites KEGG enrichment, which further confirmed the increased resistance in MoSDT1transgenic rice.…”

Section: Resultssupporting

confidence: 73%

“…Linolenic acid and linoleic acid among lipids and lipid-like molecules, raffinose among organic oxygen compounds, tyramine among benzenoids, and unclassified alphapinene showed significant accumulation at 72 h. Oxalate among organic acids and derivatives decreased at 72 h and became undetectable at the other two time points. Anthranilic acid and dopamine in benzenoids were accumulated significantly at 0 h and 120 h. Organic oxygen compounds [9], lipids and lipid-like molecules [10], benzenoids [11,12], and many unclassified metabolites were detected in M. oryzae-infected MoSDT1-transgenic resistant rice. These are the core defense metabolites in M. oryzae-infected MoSDT1-transgenic plants.…”

Section: Resultsmentioning

confidence: 99%

“…When pathogenic fungi infect plants, sugar levels in plant tissues elevate to activate plant defense responses, which participate in regulating hormonal signaling network of plant immune system and induce plants to present stronger resistance to pathogens [8]. Diverse organic oxygen compounds [9], lipids [10], benzenoids [11], and organic acids [12] play an important role in plant defense responses. Critical metabolic pathways, such as carbohydrate metabolism, amino acid metabolism, galactose metabolism, glyoxylate, and dicarboxylate metabolism, starch and sucrose metabolism, ABC transporters, etc., are activated when plants are infected by pathogens [8].…”

Section: Introductionmentioning

confidence: 99%

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Magnaporthe oryzae Systemic Defense Trigger 1 (MoSDT1)-Mediated Metabolites Regulate Defense Response in Rice

Duan

Liu

et al. 2020

Preprint

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Background: Some of the pathogenic effector proteins play an active role in stimulating the plant defense system to strengthen plant resistance.Results: In this study, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was implemented to identify altered metabolites in transgenic rice containing over-expressed M. oryzae Systemic Defense Trigger 1 (MoSDT1) that was infected at three-time points. The characterized dominating metabolites were organic acids and their derivatives, organic oxygen compounds, lipids, and lipid-like molecules. Among the identified metabolites, shikimate, galactinol, trehalose, D-mannose, linolenic acid, dopamine, tyramine, and L-glutamine are precursors for the synthesis of many secondary defense metabolites Carbohydrate, as well as amino acid metabolic, pathways were revealed to be involved in plant defense responses and resistance strengthening.Conclusion: The increasing salicylic acid (SA) and jasmonic acid (JA) content enhanced interactions between JA synthesis/signaling gene, SA synthesis/receptor gene, raffinose/fructose/sucrose synthase gene, and cell wall-related genes all contribute to defense response in rice. The symptoms of rice after M. oryzae infection were significantly alleviated when treated with six identified metabolites, i.e., galactol, tyramine, L-glutamine, L-tryptophan, α-terpinene, and dopamine for 72 h exogenously. Therefore, these metabolites could be utilized as an optimal metabolic marker for M. oryzae defense.#These authors contributed equally to this work.

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“…[19]. Moreover, the results were consistent with the defense response metabolites upregulated by Rhizoctonia solani infecting rice [9]. Table 3).…”

Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Magnaporthe oryzae Systemic Defense Trigger 1 (MoSDT1)-Mediated Metabolites Regulate Defense Response in Rice

Duan

Liu

et al. 2020

Preprint

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“…Suharti et al. developed a CE‐MS approach for the global profiling of anionic metabolites to investigate the resistance response of resistant and susceptible rice lines (32R and 29S, respectively) due to R. solani infection . Anionic metabolic profiling by CE‐MS was performed at high‐pH separation conditions using a bare fused‐silica capillary and electrophoretic separation was performed in normal polarity mode.…”

Section: Applicationsmentioning

confidence: 99%

CE‐MS for metabolomics: Developments and applications in the period 2016–2018

2018

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In the field of metabolomics, CE-MS is now recognized as a strong analytical technique for the analysis of (highly) polar and charged metabolites in a wide range of biological samples. Over the past few years, significant attention has been paid to the design and improvement of CE-MS approaches for (large-scale) metabolic profiling studies and for establishing protocols in order to further expand the role of CE-MS in metabolomics. In this paper, which is a follow-up of a previous review paper covering the years 2014-2016 (Electrophoresis 2017, 38, 190-202), main advances in CE-MS approaches for metabolomics studies are outlined covering the literature from July 2016 to June 2018. Aspects like developments in interfacing designs and data analysis tools for improving the performance of CE-MS for metabolomics are discussed. Representative examples highlight the utility of CE-MS in the fields of biomedical, clinical, microbial, and plant metabolomics. A complete overview of recent CE-MS-based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings and MS detection mode. Finally, some general conclusions and perspectives are given.

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