Metabolomic studies of anthocyanins in fruits by means of a liquid chromatography coupled to mass spectrometry workflow

Oliveira, Joana Paula da Silva; Oliveira, Renatha Tavares de; Guedes, Amanda Lima; Oliveira, Matheus da Costa; Macedo, Andrea Furtado

doi:10.1016/j.cpb.2022.100260

Cited by 6 publications

(1 citation statement)

References 186 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, in plant metabolomics experiments, biological materials are often dried to stabilize them and prevent enzymatic activities and metabolite decomposition [ 14 , 15 , 16 ]. This technique can also be applied to, e.g., dry fruits for the study of anthocyanins, which are used in food and pharmaceutical industries as natural pigments [ 17 ]; dry vegetable samples prior to NMR spectroscopy analysis [ 15 ]; prepare urine samples for headspace-SPME-GC-MS analysis of volatile organic compounds [ 18 ]; dry fecal samples to preserve microbial and chemical stability, as well as standardize samples by dry weight for gut microbiota studies [ 19 ]; and to dry tissue powder for biological and clinical research [ 20 ]. Furthermore, lyophilization can be used to reduce sample volume and to concentrate compounds in aqueous samples that would otherwise be below the limit of detection [ 21 ], e.g., plant root exudates [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Maisl,

Doppler,

Seidl

et al. 2023

Metabolites

View full text Add to dashboard Cite

Lyophilization is a common method used for stabilizing biological samples prior to storage or to concentrate extracts. However, it is possible that this process may alter the metabolic composition or lead to the loss of metabolites. In this study, the performance of lyophilization is investigated in the example of wheat roots. To this end, native and 13C-labelled, fresh or already lyophilized root samples, and (diluted) extracts with dilution factors up to 32 and authentic reference standards were investigated. All samples were analyzed using RP-LC-HRMS. Results show that using lyophilization for the stabilization of plant material altered the metabolic sample composition. Overall, 7% of all wheat metabolites detected in non-lyophilized samples were not detected in dried samples anymore, and up to 43% of the remaining metabolites exhibited significantly increased or decreased abundances. With respect to extract concentration, less than 5% of the expected metabolites were completely lost by lyophilization and the recovery rates of the remaining metabolites were slightly reduced with increasing concentration factors to an average of 85% at an enrichment factor of 32. Compound annotation did not indicate specific classes of wheat metabolites to be affected.

show abstract