Metabolomic profiling of Vitis vinifera cell suspension culture elicited with silver nitrate by 1H NMR spectrometry and principal components analysis

Choi, Hyung‐Kyoon; Yoon, Jung-Hye

doi:10.1016/j.procbio.2006.07.007

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…Chloroform extracts, obtained through the DLLME procedure, were analysed by 1D 1 H NMR to investigate metabolite variations due to different ergosterol concentration treatments. The NMR technology has been widely used as a fingerprinting tool in metabolomic analyses, with pattern recognition techniques such as PCA modelling [35] , [55] , [56] . NMR is a non-biased, non-destructive technique and one of the few analytical platforms that are rapid, reproducible, stable and require very little or no sample preparation [1] , [5] , [57] .…”

Section: Resultsmentioning

confidence: 99%

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

et al. 2014

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Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used to investigate the effect of the fungal sterol, ergosterol, on the metabolome of cultured tobacco cells. Cell suspensions were treated with different concentrations (0–1000 nM) of ergosterol and incubated for different time periods (0–24 h). Intracellular metabolites were extracted with two methods: a selective dispersive liquid-liquid micro-extraction and a general methanol extraction. Chromatographic techniques (GC-FID, GC-MS, GC×GC-TOF-MS, UHPLC-MS) and 1H NMR spectroscopy were used for quantitative and qualitative analyses. Multivariate data analyses (PCA and OPLS-DA models) were used to extract interpretable information from the multidimensional data generated from the analytical techniques. The results showed that ergosterol triggered differential changes in the metabolome of the cells, leading to variation in the biosynthesis of secondary metabolites. PCA scores plots revealed dose- and time-dependent metabolic variations, with optimal treatment conditions being found to be 300 nM ergosterol and an 18 h incubation period. The observed ergosterol-induced metabolic changes were correlated with changes in defence-related metabolites. The ‘defensome’ involved increases in terpenoid metabolites with five antimicrobial compounds (the bicyclic sesquiterpenoid phytoalexins: phytuberin, solavetivone, capsidiol, lubimin and rishitin) and other metabolites (abscisic acid and phytosterols) putatively identified. In addition, various phenylpropanoid precursors, cinnamic acid derivatives and - conjugates, coumarins and lignin monomers were annotated. These annotated metabolites revealed a dynamic reprogramming of metabolic networks that are functionally correlated, with a high complexity in their regulation.

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Section: Resultsmentioning

confidence: 99%

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

et al. 2014

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“…By using 1 H NMR coupled with PCA, a metabolomic profi ling approach has been conducted for identifying the effects of elicitation of silver nitrate in cell suspension cultures of Vitis vinifera . It was found that the metabolite profi le in an elicited suspension culture is different from the control and that lactate, alanine, acetic acid, choline, fructose, glucose, and sucrose were the key compounds for differentiation of elicited cultures from the control [53] .…”

Section: Characterization Of Metabolites In Plant Cultures and Transgmentioning

confidence: 99%

Metabolomics as a Bioanalytical Tool for Characterization of Medicinal Plants and Their Phytomedical Preparations

Happyana

Muntendam

Kayser³

2012

Pharmaceutical Biotechnology

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“…The huge amount of data provided is generally analysed using multivariate data analysis (MVDA). This combination allows one to get a good visualization of the dataset and has already been applied to the metabolic profiling of various plant species (Ward et al, 2003;Choi et al, 2004a-c;Kim, et al, 2005;Abdel-Farid et al, 2007;Choi and Yoon, 2007), but also to wine (Brescia et al, 2002), coffee (Charlton et al, 2002), fruit juice (Vogels et al, 1996) and beer (Duarte et al, 2002). In vanilla, NMR-based metabolomic profiles of V. planifolia green pods, leaves and organogenic calli were characterized, but we will discuss here only the results on green pods and leaves.…”

Section: Vanilla Metabolomicsmentioning

confidence: 99%

Metabolomic Analysis of a Commercially Important Aromatic Plant from the Indian Ocean: Vanilla planifolia

Palama

2014

Novel Plant Bioresources

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Metabolomic profiling of Vitis vinifera cell suspension culture elicited with silver nitrate by 1H NMR spectrometry and principal components analysis

Cited by 10 publications

References 18 publications

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

Multi-Platform Metabolomic Analyses of Ergosterol-Induced Dynamic Changes in Nicotiana tabacum Cells

Metabolomics as a Bioanalytical Tool for Characterization of Medicinal Plants and Their Phytomedical Preparations

Metabolomic Analysis of a Commercially Important Aromatic Plant from the Indian Ocean: Vanilla planifolia

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