Metabolomic profiling in tomato reveals diel compositional changes in fruit affected by source–sink relationships

Bénard, Camille; Bernillon, Stéphane; Biais, Benoît; Osorio, Sonia; Maucourt, Mickaël; Ballias, Patricia; Deborde, Catherine; Colombié, Sophie; Cabasson, Cécile; Jacob, Daniel; Vercambre, Gilles; Gautier, Hélène; Rolin, Dominique; Génard, Michel; Fernie, Alisdair R.; Gibon, Yves; Moing, Annick

doi:10.1093/jxb/erv151

Cited by 68 publications

(62 citation statements)

References 66 publications

(84 reference statements)

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“…In apples and tomatoes, the role of sucrose in sink-strength and source-sink assimilate translocation was suggested as secondary to that of sorbitol and starch, respectively (F. Wang, Sanz, Brenner, & Smith, 1993;Watari et al, 2004). Correspondingly, diurnal changes in the level of starch in tomatoes were found to be significant (Benard et al, 2015). Here, we found that berries lost between 2.3 and 3.5 mg g −1 fresh weight of sucrose during the day, equivalent to roughly 20%-25% of their maximum daily level of sucrose, present during early morning.…”

Section: Discussionmentioning

confidence: 99%

Grape berry position affects the diurnal dynamics of its metabolic profile

Reshef

Fait

Agam

2019

Plant Cell & Environment

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Solar irradiance and air temperature are characterized by dramatic circadian fluctuations and are known to significantly modulate fruit composition. To date, it remains unclear whether the abrupt, yet predictive, diurnal changes in radiation and temperature prompt direct metabolic turn‐over in the fruit. We assessed the role of fruit insolation, air temperature, and source‐tissue CO2 assimilation in the diurnal compositional changes in ripening grape berries. This was performed by comparing the diurnal changes in metabolite profiles of berries positioned such that they experienced (a) contrasting diurnal solar irradiance patterns, and (b) similar irradiance but contrasting diurnal CO2 assimilation patterns of adjacent leaves. Grape carbon levels increased during the morning and decreased thereafter. Sucrose levels decreased throughout the day and were correlated with air temperature, but not with the diurnal pattern of leaf CO2 assimilation. Tight correlation between sucrose and glucose‐6‐phosphate indicated the involvement of photorespiration/glycolysis in sucrose depletion. Amino acids, polyamines, and phenylpropanoids fluctuated diurnally, and were highly responsive to the diurnal insolation pattern of the fruit. Our results fill the knowledge gap regarding the circadian pattern of source‐sink assimilate‐translocation in grapevine. In addition, they suggest that short‐term direct solar exposure of the fruit impacts both its diurnal and nocturnal metabolism.

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Section: Discussionmentioning

confidence: 99%

Grape berry position affects the diurnal dynamics of its metabolic profile

Reshef

Fait

Agam

2019

Plant Cell & Environment

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“…Fumarate, free galactose and galacturonate were quantified using 1 H‐NMR analyses of polar extracts issued from 20 mg of lyophilized powder as described previously (Bénard et al ., ). Metabolite concentrations were calculated using selected peak areas and calibration curve data.…”

Section: Methodsmentioning

confidence: 97%

Respiration climacteric in tomato fruits elucidated by constraint‐based modelling

et al. 2016

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Summary Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with the brief phase of accelerated ripening associated with the respiration climacteric because of practical problems involved in measuring fruit respiration.Because constraint‐based modelling allows predicting accurate metabolic fluxes, we investigated the respiration and energy dissipation of fruit pericarp at the breaker stage using a detailed stoichiometric model of the respiratory pathway, including alternative oxidase and uncoupling proteins. Assuming steady‐state, a metabolic dataset was transformed into constraints to solve the model on a daily basis throughout tomato fruit development.We detected a peak of CO 2 released and an excess of energy dissipated at 40 d post anthesis (DPA) just before the onset of ripening coinciding with the respiration climacteric. We demonstrated the unbalanced carbon allocation with the sharp slowdown of accumulation (for syntheses and storage) and the beginning of the degradation of starch and cell wall polysaccharides. Experiments with fruits harvested from plants cultivated under stress conditions confirmed the concept.We conclude that modelling with an accurate metabolic dataset is an efficient tool to bypass the difficulty of measuring fruit respiration and to elucidate the underlying mechanisms of ripening.

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“…Some of the main objectives of metabolomic studies in agriculture are as follows: know the metabolic responses towards any type of stress; generate metabolic proiles for genetic mapping and generate metabolic proiles to study the impact of Sugars, amino acids, organic acids/NMR, GC-TOF-MS Growth and development [49] Organic acids/NMR, GC-MS Metabolic engineering [89] Enzymes/GC-MS Fruit development in transgenic plants [90] Maize Amino acids, carbohydrates, organic acids/NMR Salt stress [27] Total nitrogen, protein/MS-MS Development and growth [85] Protein/MALDI-TOF-MS Metabolic changes under phosphorus deicit [26] Potato Glycoalkaloids, fructans/GC-TOF-MS, LC-MS Genetically modiied plants [37] Faty acids, amino acids, organic acids/NMR, HPLC-UV Genetic modiications to metabolic pathways [39] Sugars, amino acids, organic acids/FIE-MS, GC-MS Total composition analysis and quality…”

Section: Applications Of Metabolomics In Agriculturementioning

confidence: 99%

“…During the day, any plant species has changes in its chemical composition due to the action or efect of the environment, something that [49] determined when detected 70 metabolites in tomato leaves and 60 in the fruit using NMR and MS. Also, with the aid of metabolomics, it is possible to identify particular compounds with some speciic function, important to the survival of a species. With respect to this, 1-methyltryptophan was identiied as the metabolite involved in the response of the plant to Botrytis cinerea and Pseudomonas syringae [50].…”

Section: Metabolomics -Fundamentals and Applicationsmentioning

confidence: 99%

Metabolomics as a Tool in Agriculture

Ibarra-Estrada¹,

Soto-Hernández²,

Palma-Tenango³

2016

Metabolomics - Fundamentals and Applications

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Metabolomics is a study through can be obtain a beter understanding of the complexity of the biological systems, through the chemical composition and relations with the physiology of the plant. The literature describes a lot of information on this support in areas of medicinal plants, chemosystematics, adulteration of plants, etc., but it is scarce in agriculture. At present, agriculture plays a crucial role in human beings. The demand of foods has increased due to the continuous increase in the population, and this requires an increase in the production of crops, besides the crops being afected by the climatic change, atack of pest and diseases and resistance to a conventional agrochemicals. At present, scientists are doing some practices or studies of genetic improvement of crops to increase their production and avoid the problems pointed out. It is an important part of the genomic studies; the results could be the basis for a genetic improvement based on the chemical composition of the crops, and in the metabolomics studies represent a crucial role in their quality for human consumption. The aim of the chapter is to review the literature from 10 years behind emphasizing the importance of metabolomics in crops of economic and feed value.

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Metabolomic profiling in tomato reveals diel compositional changes in fruit affected by source–sink relationships

Abstract: HighlightUsing metabolomics in tomato, we confirmed the existence of diel patterns in leaf composition and showed lower but significant diel changes in expanding fruit depending on the potential carbon supply.

Cited by 68 publications

References 66 publications

Grape berry position affects the diurnal dynamics of its metabolic profile

Grape berry position affects the diurnal dynamics of its metabolic profile

Respiration climacteric in tomato fruits elucidated by constraint‐based modelling

Metabolomics as a Tool in Agriculture

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