Metabolomic profiling in evaluation of cultivated oat species with different ploidy level

Abstract

The article presents biochemical characteristics identified during the analysis of metabolomic profiles. The object of this study was the landraces of cultivated oat species Avena strigosa Schreb., A. abyssinica Hochst. and A. sativa L. with different ploidy levels from the global collection of the Department of Genetic Resources of Oats, Rye, and Barley of VIR. Metabolic profiling was performed using an Agilent 6850 gas chromatograph (Agilent Technologies, USA). The main task of this evaluation was determination of differences between cultivated oat species of diploid, tetraploid and hexaploid ploidy levels according to their metabolomic spectra. These spectra reflect the metabolic state of genotypes of various ecological and geographical origin. The investigation touched on the most important groups of metabolites important for the resistance formation nutritional, medicinal and dietary benefits. Particular, attention was paid to biologically active compounds that determine the functional value of daily human foods − phenolic compounds, free amino acids and sugar acids. The most informative indicators, which separate the oat with different ploidy levels were: isofucosterol, xylitol, MAG-1 18: 0, linolenic, undecylic, threonic, glutamic and methylmalonic acids.

Metabolomic approach to investigate <i>Dactylis glomerata</i> L. from the VIR collection

“…That was taken into account when we distinguished economically significant D. glomerata accessions. The same compounds dominate among organic acids, oligosaccharides, phytosterols and PhC in the MP of cocksfoot and oat seedlings and green mass of peavine previously studied by us (Solovyeva et al, 2019(Solovyeva et al, , 2020Loskutov et al, 2021). In the MP of oat and cocksfoot seedlings in the group of polyols and FA, the main substances are isomers of inositol and linoleic and palmitic acids, peavine and cocksfoot -glycosides: methylglucoside (Loskutov et al, 2021).…”

“…The same compounds dominate among organic acids, oligosaccharides, phytosterols and PhC in the MP of cocksfoot and oat seedlings and green mass of peavine previously studied by us (Solovyeva et al, 2019(Solovyeva et al, , 2020Loskutov et al, 2021). In the MP of oat and cocksfoot seedlings in the group of polyols and FA, the main substances are isomers of inositol and linoleic and palmitic acids, peavine and cocksfoot -glycosides: methylglucoside (Loskutov et al, 2021). An iridoid glycoside -antirrhinoside was detected in both the green mass of peavine and cocksfoot (Solovyeva et al, 2019(Solovyeva et al, , 2020.…”

OMICS in Fodder Crops: Applications, Challenges, and Prospects

“…It contains minerals, proteins, fiber, vitamins, lipids, unsaturated fatty acids, as well as other biochemical compounds that play a role in preventing diseases such as colon cancer, type II diabetes, cardiovascular disorders, etc. [ 184 ]. Cultivated oat ( Avena sativa L.) is an allohexaploid (2 n = 6x = 42; 1C-value = 13.2 (pg) DNA) with three diploid sub-genomes (AA, CC, DD).…”

“…The metabolomic profiles of different oat species ( Avena strigosa Schreb (diploid), A. abyssinica Hochst (tetraploid), and A. sativa L. (hexaploid)) give the informative indicators for separation of these species at different ploidy levels. Metabolites such as xylitol, undecylic, glutamic acid, isofucosterol, MAG-118:0, linolenic, methylmalonic, and undecylic acid were identified using an Agilent 6850 gas chromatograph and can be used as informative indicators [ 184 ]. A study performed by Pretorious and coworkers where used metabolomics for the identification of signature biochemical compounds that can be used for developing the differential metabolic profiles of various oat cultivars (Dunnart, Magnifico, Pallinup, SWK001, and Overberg).…”