Metabolomic profiling highlights oxidative damages in platelet concentrates treated for pathogen inactivation and shows protective role of urate

Abonnenc, Mélanie; Crettaz, David; Marvin, Laure; Grund, Baptiste; Sonego, Giona; Bardyn, Manon; Tissot, Jean‐Daniel; Prudent, Michel; Rochat, Bertrand; Lion, Niels

doi:10.1007/s11306-016-1136-0

Cited by 20 publications

(23 citation statements)

References 52 publications

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“…C and D). We speculate that the diminished response of PCs to ADP observed on Day 1 might be due to an exposure of PLTs to ADP, either leaking from the cells during processing or due to residual ADP from contaminant RBCs, even though ADP has not been reported in PC supernatant in recent metabolomic analyses . Our hypothesis is supported by the fact that mepacrine load is similar between donor PRP and PCs, confirming an unchanged content of dense granules (Fig.…”

Section: Discussionsupporting

confidence: 69%

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Generation of procoagulant collagen‐ and thrombin‐activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage

et al. 2018

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Section: Discussionsupporting

confidence: 69%

“…During storage at 22°C up to 7 days under agitation, various lesions affect PLT activation, metabolism, and PLT morphology . Some of these changes may be reversible upon transfusion while others result in the accelerated clearance of PLTs from the recipient's circulation.…”

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confidence: 99%

Generation of procoagulant collagen‐ and thrombin‐activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage

et al. 2018

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“…However, as storage proceeded, the decreasing pH of the supernatant may trigger the influx of UA into the stored RBCs, lowering thus the intracellular ROS levels . Such a protective role of UA has been also shown in platelet (PLT) concentrates where addition of urate in excess leads to lower ROS production . Moreover, the UA present in the uremic plasma exerts a strong antioxidant effect compared to healthy plasma on both healthy RBCs and RBCs from subjects with severe renal disease in vitro, while its levels can effectively predict the RBC life span in chronic hemodialysis patients …”

Section: Discussionmentioning

confidence: 99%

“…16 Such a protective role of UA has been also shown in platelet (PLT) concentrates where addition of urate in excess leads to lower ROS production. 17 Moreover, the UA present in the uremic plasma exerts a strong antioxidant effect compared to healthy plasma on both healthy RBCs and RBCs from subjects with severe renal disease in vitro, 18 while its levels can effectively predict the RBC life span in chronic hemodialysis patients. 19 Further end-of-storage diversification of the blood units, in favor of the high-UA ones, include the interrelated variables of intracellular calcium accumulation and severe shape modifications that can be also associated with a better antioxidant capacity.…”

Section: Serum Ua Classifies the Rbcs According To Their Storability mentioning

confidence: 99%

Donor‐specific individuality of red blood cell performance during storage is partly a function of serum uric acid levels

et al. 2017

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BACKGROUND Previous investigations in leukoreduced units of red blood cells (RBCs) in mannitol additive solution revealed the close association of uric acid (UA) levels in vivo with the susceptibility of RBCs to storage lesion markers. In this study, we examined whether UA has a similar correlation with the capability of RBCs to cope with the oxidative provocations of storage under different conditions, namely, in CPDA‐1 and in the absence of leukoreduction. STUDY DESIGN AND METHODS The UA‐dependent antioxidant capacity of the supernatant was measured in nonleukoreduced units of RBCs in CPDA (n = 47). The possible effect of UA variability on the storage lesion profile was assessed by monitoring several physiologic properties of RBCs and supernatant, including cell shape, reactive oxygen species, and size distribution of extracellular vesicles, in units exhibiting the lowest or highest levels of UA activity (n = 16) among donors, throughout the storage period. RESULTS In stored RBC units, the UA‐dependent antioxidant activity of the supernatant declined as a function of storage duration but always in strong relation to the UA levels in fresh blood. Contrary to units of poor‐UA activity, RBCs with the highest levels of UA activity exhibited better profile of calcium‐ and oxidative stress–driven modifications, including a significant decrease in the percentages of spherocytes and of 100‐ to 300‐nm‐sized vesicles, typically associated with the exovesiculation of stored RBCs. CONCLUSION The antioxidant activity of UA is associated with donor‐specific differences in the performance of RBCs under storage in nonleukoreduced CPDA units.

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“…It has been observed that UV light as part of PI treatment increases PLT storage lesions . The molecular basis of PLT storage lesions includes altered metabolic support, increase in PLT activation markers, accumulation of bioactive substances, morphological changes, and mitochondrial dysfunction; it may be associated with decreased posttransfusion survival . However, these impaired functions are not reported, are reported to a lower degree in other studies, or depend on the pathogen reduction (PR) technique used…”

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confidence: 99%

Influence of platelet preparation techniques on in vitro storage quality after psoralen‐based photochemical treatment using new processing sets for triple‐dose units

Lotens¹,

Valensart²,

Najdovski³

et al. 2018

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BACKGROUND: The INTERCEPT Blood System (IBS)for platelets (PLTs) uses a combination of psoralen and ultraviolet-A light to inactivate pathogens that may contaminate PLT concentrates (PCs). However, no data are available on the quality of IBS-treated PLTs from different apheresis and buffy-coat PC preparation platforms using the new triple storage (TS) set. STUDY DESIGN AND METHODS:The objective of this study was to evaluate the TS set on three different preparation platforms compared with the large-volume (LV) set, as control. PLT in vitro metabolic and activation parameters were studied over 7 days.RESULTS: Several statistical differences are observed between the two sets, particularly for pH, oxygen pressure (pO 2 ), carbonic gaz pressure (pCO 2 ), and bicarbonate. The three different preparation techniques influence PLT parameters, and the difference is statistically significant for all the studied parameters, except for pCO 2 . The TS set has the advantage of shorter compound adsorption device time, higher PLT recoveries, and less PLT activation. ABBREVIATIONS: APC = apheresis platelet concentrate; ATP = adenosine triphosphate; BC = buffy-coat; BCPC = buffy-coat platelet concentrate; CAD = compound adsorption device; ELISA = enzymelinked immunosorbent assay; HCO 3 = bicarbonate; IBS = INTERCEPT Blood System; LDH = lactate dehydrogenase; LV = large volume; PAS = platelet additive solution; PC(s) = platelet concentrate(s); PI = pathogen inactivation; PLT = platelet; TACSI PL = Terumo automated centrifuge and separator integration platelet; TS = triple storage; UV = ultraviolet.From the

show abstract

Metabolomic profiling highlights oxidative damages in platelet concentrates treated for pathogen inactivation and shows protective role of urate

Cited by 20 publications

References 52 publications

Generation of procoagulant collagen‐ and thrombin‐activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage

Generation of procoagulant collagen‐ and thrombin‐activated platelets in platelet concentrates derived from buffy coat: the role of processing, pathogen inactivation, and storage

Donor‐specific individuality of red blood cell performance during storage is partly a function of serum uric acid levels

Influence of platelet preparation techniques on in vitro storage quality after psoralen‐based photochemical treatment using new processing sets for triple‐dose units

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