Metabolomic profiling and biological investigation of Tabebuia Aurea (Silva Manso) leaves, family Bignoniaceae

Abstract

Both ethyl acetate and aqueous fractions of Tabebuia aurea leaves exhibited noteworthy antioxidant and nephroprotective activities against carbon tetrachloride (CCl 4 )-induced nephrotoxicity in rats, as evidenced by the remarkable improvements of renal serum biomarkers and histopathological features. Additionally, the ethyl acetate fraction displayed a prominent in vitro antitrypanosomal activity against Trypanosoma brucei; consequently, the leaves were subjected to LC-HR-ESI-MS metabolomic profiling to discover the constituents that possibly underlie their bioactivities. Therefore, ten metabolites were characterized, mostly dominated by flavonoids.Interestingly, two identified constituents viz., 3,9,12,15-octadecatetraenoic acid (9) and 9,11,13-octadecatrienoic acid (10) are reported firstly herein from the genus Tabebuia. Furthermore, among the dereplicated constituents, rutin (5) and kaempferol 3-O-rutinoside (6) exhibited the highest docking scores as effective antitrypanosomal compounds.

Metabolic profiling of cytotoxic metabolites from five Tabebuia species supported by molecular correlation analysis

“…“For our quality check, as an alternative to an internal standard to monitor the alignment of peaks, we used rutin (C 27 H 30 O 16 , m/z 609.1459 [M−H]-, tR 10.65), which is an universal endogenous plant metabolite, and which belongs to more than 30 plant families. Moreover, we have reported the presence of rutin from Tabebuia aurea in our previous work 20 . The figure (S1) shows the retention time alignment of rutin’s peak in all samples, which was within the acceptable RT tolerance threshold according to our previous work 21 .…”

“…The dereplication was achieved for each m/z ion peak with metabolites recorded in the customized databases based on established parameters (m/z threshold of ± 3 ppm and retention time), consequently, the number of the remaining unknown metabolites in each species was refine. The raw data was processed, aligned and merged into one dataset according to the method previously developed in our lab 20 , 22 , 37 .…”

Anti-androgenic potential of the fruit extracts of certain Egyptian Sabal species and their genetic variability studies: a metabolomic-molecular modeling approach

“…Both positive and negative ionization modes were applied to detect the maximum possible number of metabolites. [29][30][31][67][68][69] The dereplication study relied on a precise molecular formula prediction and chemotaxonomic filtration to minimize the number of hits per molecular formula, which led to the tentative identification of the top hits. 20,70 The Food & Function Paper 3.5.…”

Flavonoidal glycosides and in vitro antioxidant activity of Bignonia binata Thunb. leaves Family Bignoniaceae and in silico evidence of their potential anti-COVID-19 activity.

“…We prepared the control by using MeOH instead of the sample and the absorbance was measured (3 times) at 517 nm. The concentration, which reduced half of the initial DPPH − concentration (EC50) was calculated from the graph of DPPH scavenging effect against sample concentration [11].…”

“…After cooling, the absorbance was measured at 695 nm against a blank (3 times). The calibration curve was made using ascorbic acid, so that the antioxidant activity was expressed in mg ascorbic acid equiv./g dried sample [11].…”