Metabolomic profiles of tropical Chlorella species in response to physiological changes during nitrogen deprivation

Vello, Vejeysri; Chu, Wan-Loy; Lim, Phaik-Eem; Majid, Nazia Abdul; Phang, Siew‐Moi

doi:10.1007/s10811-018-1504-4

Cited by 17 publications

(9 citation statements)

References 83 publications

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“…Generally, N− had a big impact on the chloroplast composition: not only the chlorophyll, but also the carotenoids decreased [54][55][56][57][58] and decline of chloroplast lipid content occurred [14]. The photosynthetic electron flow was rebalanced in N− cells, indicating that the decrease in Fv/Fm values in N− cells may be part of the adaptation process to N− to relocate the nitrogen to the essential cellular metabolism process [57,59]. Intriguingly, previous research on the transcriptome suggested that all the LHCs were downregulated, including both PSI and PSII systems [53,54,58].…”

Section: The Change Tendencies Of Lhc Proteins and Upregulated Ccm Onmentioning

confidence: 99%

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

You

Wei

Gong

et al. 2020

Biotechnol Biofuels

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Background: Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipids in the form of triacylglycerides (TAG). Mechanisms of this process from the perspective of transcriptome and metabolome have been obtained previously, yet proteome analysis is still sparse which hinders the analysis of dynamic adaption to nitrogen deficiency. Here, proteomes for 3 h, 6 h, 12 h, 24 h, 48 h and 10th day of nitrogen deplete (N−) and replete (N+) conditions were obtained and integrated with previous transcriptome data for N. oceanica. Results: Physiological adaptations to N− not apparent from transcriptome data were unveiled: (a) abundance of proteins related to photosynthesis only slightly decreased in the first 48 h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48 h under N−, suggesting that respiration is enhanced after 48 h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48 h, synchronous with the previously reported diversification of fatty acids after 48 h. Conclusions: This study adds a proteome perspective on the major pathways for TAG accumulation in Nannochloropsis spp. Temporal changes of proteome exhibited distinct adaptation phases that are usually delayed relative to transcriptomic responses. Notably, proteome data revealed that photosynthesis and carbon fixation are still ongoing even after 48 h of N−. Moreover, sometimes completely opposite trends in proteome and transcriptome demonstrate the relevance of underexplored post-transcriptional regulation for N− adaptation.

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Section: The Change Tendencies Of Lhc Proteins and Upregulated Ccm Onmentioning

confidence: 99%

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

You

Wei

Gong

et al. 2020

Biotechnol Biofuels

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“…This was because S. obliquus was cultured under nitrogen-starvation conditions in the present study. In the absence of nitrogen, reallocation of membrane lipids will induce microalgal cells, especially cells with fatty acids C16:0 and C18:1, to accumulate lipids (Vello et al 2018). In this study, when the aeration rate was 0.9 L min −1 , the transportation of nitrogen was restricted, leading to S. obliquus absorbing less nitrogen and accumulating more C16-C18 fatty acids.…”

Section: Fatty Acid Composition Under Different Aeration Ratesmentioning

confidence: 76%

“…It has been shown previously that the reduction in photosynthesis caused by nitrogen deficiency leads to a change in the metabolic pathways involved in carbon and nitrogen assimilation and metabolism (Yang et al 2014). In the absence of nitrogen, metabolic reprogramming of central carbon metabolism occurs, which transfers carbon flow to lipid synthesis (Vello et al 2018). Hamid and Sibi (2019) found that there was a 63% increase in the lipid content of Chlorococcopsis minuta in nitrogen-deficient conditions compared with nitrogen-sufficient conditions.…”

Section: Content and Productivity Of Lipid Protein And Polysaccharimentioning

confidence: 95%

Recommended turbulent energy dissipation rate for biomass and lipid production of Scenedesmus obliquus in an aerated photosynthetic culture system

Yang

et al. 2020

Environ Sci Pollut Res

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Effects of turbulent energy dissipation rate (increased from 1.28 × 10 −6 to 1.67 × 10 −5 m 2 s −3) on Scenedesmus obliquus biomass and lipid accumulation at different aeration rates (0.3, 0.6, 0.9, 1.2, and 1.5 L min −1) were investigated. The turbulent energy dissipation rate was calculated by CFD model simulation. When the turbulent energy dissipation rate increased to 7.30 × 10 −6 m 2 s −3 , the biomass and lipid productivity increased gradually, and finally reached their maximum values of 1.11 × 10 7 cells mL −1 and 16.0 mg L −1 day −1 , respectively. When it exceeded 7.30 × 10 −6 m 2 s −3 , the biomass and lipid productivity showed a decreasing trend. Therefore, the most favorable turbulent energy dissipation rate for S. obliquus growth and lipid accumulation was 7.30 × 10 −6 m 2 s −3 .

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“…Generally, N-had a big impact on the chloroplast composition: not only the chlorophyll, but also the carotenoids decreased [55][56][57] and decline of chloroplast lipid content occurred [14]. The photosynthetic electron flow was rebalanced in N-cells, indicating that the decrease in Fv/Fm values in N-cells may be part of the adaptation process to N-to relocate the nitrogen to the essential cellular metabolism process [56,58]. Intriguingly, previous research on the transcriptome suggested that all the LHCs were downregulated, including both PSI and PSII systems [52,53,57].…”

Section: Discussionmentioning

confidence: 99%

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

You

Wei

Gong

et al. 2019

Preprint

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Background Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipid in the form of triacylglycerides (TAG). Several researches have studied the mechanism of this process from the perspective of transcriptome and metabolome, yet proteome analysis on this process is still sparse and lacking the analysis of the dynamic adaption to nitrogen deficiency. Here, proteomes for 03h, 06h, 12h, 24h, 48h and 10th day of nitrogen deplete and replete conditions were compared. New proteome results were integrated with existing transcriptome and other data.Results Obtained results illuminated physiological adaptations not deduced from previous transcriptome data: (a) Abundance of proteins related to photosynthesis only slightly decreased in the first 48h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48h under nitrogen deficiency, suggesting that respiration is enhanced after 48h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48h, synchronous with the previously reported diversification of fatty acids after 48h.Conclusions This study adds a proteome perspective on the major pathways for TAG accumulation by Nannochloropsis due to absence of nitrogen: photosynthesis, membrane lipid conversion, protein degradation, TCA cycle. By integrating existing transcriptome and other data, our research provided for Nannochloropsis oceanica a multi-layered description of adaptation to nitrogen limitation and lipid accumulation. Cluster analysis of this integrated dataset allowed inference of post-transcriptional regulation events.

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Metabolomic profiles of tropical Chlorella species in response to physiological changes during nitrogen deprivation

Cited by 17 publications

References 83 publications

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

Recommended turbulent energy dissipation rate for biomass and lipid production of Scenedesmus obliquus in an aerated photosynthetic culture system

Integration of proteome and transcriptome refines key molecular processes underlying oil production in Nannochloropsis oceanica

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