Metabolomic profile of young male goats seropositive to small ruminant lentivirus – A longitudinal study

Profile of serum lipid metabolites of one-week-old goat kids depending on the type of rearing

“…Therefore, direct comparisons with our results are difficult. In general, profile of serum lipid metabolites of one-week-old goat kids appears to be similar to the limited profile of adult goats which we investigated in our previous studies [ 15 , 16 ] as well as to the profile of other ruminant species, except for the concentration of triacylglycerols which seems to be much higher than in adults goats [ 19 ], sheep and cattle [ 20 , 21 ]. This is likely a direct consequence of intensive feeding with either milk or milk replacer, which both contain high concentrations of triacylglycerols [ 22 ].…”

“…This assay covers over 500 lipid metabolites. This range of metabolites is much wider than covered by the AbsoluteIDQ® p180 Kit, which we used in our previous studies dedicated to the metabolomic profile of adult goats with SRLV infection [ 15 , 16 ]. The MxP® Quant 500 Kit allows to investigate 8 additional lipid classes – ceramides, dihydroceramides, hexosylceramides, dihexosylceramides, trihexosylceramides, cholesteryl esters, diglycerides, and triacylglycerols.…”

“…Over the last decade metabolomics has made a considerable progress in investigating various aspects of livestock production and diseases, which has led to identification of biomarkers of bovine transition diseases such as mastitis, metritis, laminitis, hypocalcemia, ketosis [ 9 , 10 ], displaced abomasum [ 11 ] and hepatic lipidosis [ 12 ] as well as production traits in dairy [ 13 ] and beef cattle [ 14 ]. On the other hand, metabolomic studies in small ruminants lagged far behind, with only a few studies so far carried out and focused on limited set of metabolites in adult male goats [ 15 ] and female goats [ 16 ] and sheep [ 17 ]. This situation resulted from the lack of widely available methods of quantification of most important metabolites on one side, and from the lesser role played by sheep and goats in the industry of most of countries on the other.…”

“…This is particularly important as a considerable part or in some conditions even the vast majority of biologically significant biomarkers have proven to be lipid metabolites [ 12 ]. Some recent studies in small ruminants quantified a limited spectrum of lipid metabolites in adult goats [ 15 , 16 ], however the physiological concentration of them remains unknown in young small ruminants [ 18 ].…”