Metabolomic Changes after Coffee Consumption: New Paths on the Block

Abstract

ScopeSeveral studies suggest that regular coffee consumption may help preventing chronic diseases, but the impact of daily intake and the contribution of coffee metabolites in disease prevention are still unclear. The present study aims at evaluating whether and how different patterns of coffee intake (one cup of espresso coffee/day, three cups of espresso coffee/day, and one cup of espresso coffee/day and two cocoa‐based products containing coffee two times per day) may impact endogenous molecular pathways.Methods and ResultsA three‐arm, randomized, crossover trial is performed in 21 healthy volunteers who consumed each treatment for one month. Urine samples are collected to perform untargeted metabolomics based on UHPLC‐IMS‐HRMS. A total of 153 discriminant metabolites are identified. Several molecular features are associated with coffee consumption, while others are linked with different metabolic pathways, such as phenylalanine, tyrosine, energy metabolism, steroid hormone biosynthesis, and arginine biosynthesis and metabolism.ConclusionThis information has provided new insights into the metabolic routes by which coffee and coffee‐related metabolites may exert effects on human health.

Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study

“…As already reported [ 25 , 26 , 27 ], coffee capsules were chosen based on the results of a previous paper [ 2 ], while the amount of cocoa-based confectionery containing coffee was decided in order to provide approximately half of the caffeine content than the coffee.…”

“…In light of these controversial observations, there is a clear need to perform dietary intervention studies devoted to elucidating the impact of coffee consumption on human health. Within this context, the Pocket-4-life project [ 24 ] was developed with the specific purpose of investigating the bioavailability of the bioactive compounds of espresso coffee and cocoa-based confectionery containing coffee [ 25 , 26 ], as well as to study the potential beneficial effect of these products on cardiometabolic and oxidative stress markers [ 24 , 27 ]. Specifically, within the Pocket-4-Life project, we investigated the effect of one-month consumption of different doses of coffee or of cocoa-based products containing coffee on markers of oxidative stress, such as DNA damage and lipid peroxidation.…”

LC–MS Characterization and Quantification of Known and Unknown (Poly)phenol Metabolites—Possible Pitfalls and Their Avoidance

“…An untargeted metabolomic study provides the only report of urine metabolites as eight urinary C 6 –C 5 phase II‐conjugates with one double bond in the side chain, [ 79 ] i.e., the masses are 2 amu less than the well‐known analogues with a saturated side chain. [ 80 ] These could be the well documented phase‐II conjugates of substituted 5‐phenyl‐valerolactones, [ 80 ] the deficit of 2 amu explained by an additional phenyl ring hydroxyl (+16 amu) and the loss of water (–18 amu) during lactonization ( Figure 4 ).…”

Nutritional Therapy in Persons Suffering from Psoriasis

“…Recent studies [ 80 , 89 ] demonstrate that the effect of coffee is dose-dependent. Regular moderate coffee consumption (up to 3 cups per day) alleviates psoriasis symptoms and has an anti-inflammatory effect, whereas higher coffee consumption (especially >4 cups of coffee per day) exacerbates clinical symptoms of psoriasis, which is associated with an increase in pro-inflammatory substances.…”