Metabolomic approach of the antiprotozoal activity of medicinal Piper species used in Peruvian Amazon

Vásquez-Ocmín, Pedro; Gadea, Alice; Cojean, Sandrine; Marti, Guillaume; Pomel, Sébastien; Baelen, Anne-Cécile Van; Ruiz-Vásquez, Liliana; Figadère, Bruno; Mesia, Lastenia Ruiz; Maciuk, Alexandre

doi:10.1016/j.jep.2020.113262

Cited by 11 publications

(21 citation statements)

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“…The Piperaceae family includes a large number of plants used in tropical and subtropical regions. The Piper and Peperomia genera are the most representative of this botanical family and Piper species are commonly used in Latino-American traditional medicines for the treatment of protozoal diseases [ 15 ]. From the Piperaceae family, some species have been tested against T. cruzi and some bioactive compounds have been isolated such as flavonoids, terpenoids, lignans, chromanes, and alkaloids [ 13 , 15 , 16 ].…”

Section: Resultsmentioning

confidence: 99%

Anti-trypanosomal screening of Salvadoran flora

et al. 2021

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Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and in Central America, it is considered one of the four most infectious diseases. This study aimed to screen the anti-trypanosomal activity of plant species from Salvadoran flora. Plants were selected through literature search for plants ethnobotanically used for antiparasitic and Chagas disease symptomatology, and reported in Museo de Historia Natural de El Salvador (MUHNES) database. T. cruzi was incubated for 72 h with 2 different concentrations of methanolic extracts of 38 species, among which four species, Piper jacquemontianum, Piper lacunosum, Trichilia havanensis, and Peperomia pseudopereskiifolia, showed the activity (≤ 52.0% viability) at 100 µg/mL. Separation of the methanolic extract of aerial parts from Piper jacquemontianum afforded a new flavanone (4) and four known compounds, 2,2-dimethyl-6-carboxymethoxychroman-4-one (1), 2,2-dimethyl-6-carboxychroman-4-one (2), cardamomin (3), and pinocembrin (5), among which cardamomin exhibited the highest anti-trypanosomal activity (IC50 = 66 µM). Detailed analyses of the spectral data revealed that the new compound 4, named as jaqueflavanone A, was a derivative of pinocembrin having a prenylated benzoate moiety at the 8-position of the A ring. Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Anti-trypanosomal screening of Salvadoran flora

et al. 2021

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“…The Amazon region has a rich biodiversity that includes a large number of plant species that are commonly used in traditional medicine and have contributed to the discovery of several bioactive species [7]. Studies have increased and greater emphasis has been placed on Amazonian species of phytochemical interest [7,8]. Among these species with pharmacological potential, those of the genus Piper stand out, due to their use in traditional medicine and promising biological activities [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Studies have increased and greater emphasis has been placed on Amazonian species of phytochemical interest [7,8]. Among these species with pharmacological potential, those of the genus Piper stand out, due to their use in traditional medicine and promising biological activities [8,9]. Piper is the largest genus of the family Piperaceae, with nearly 2000 species of herbs, shrubs, or small trees distributed throughout the tropical and subtropical forests, primarily in Central and South America, the Caribbean, Africa, Asia, and the Pacific Islands [10].…”

Section: Introductionmentioning

confidence: 99%

“…Piper is the largest genus of the family Piperaceae, with nearly 2000 species of herbs, shrubs, or small trees distributed throughout the tropical and subtropical forests, primarily in Central and South America, the Caribbean, Africa, Asia, and the Pacific Islands [10]. In fact, these plants have been commonly used in traditional medicine [8,[11][12] and are an important source of therapeutic agents [13], as they display a great chemical diversity of secondary metabolism [10,14], including alkaloids [15], flavonoids [16], terpenoids [17], phenylpropanoids [18], phenolic acids [19] and lignans [20]. These constituents have been reported to have antioxidant [15], antiinflammatory…”

Section: Introductionmentioning

confidence: 99%

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Antileishmanial Activity of the Genus Piper: A Systematic Review

Macêdo

Castro

Silva

2021

EJMP

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Background: Leishmaniases are anthropozoonotic vector-borne diseases, caused by the protozoan Leishmania spp. These diseases have significant morbidity and mortality worldwide and, as there are currently no vaccines available for their treatment, chemotherapy remains the mainstay for anti-leishmanial therapeutics. However, the severe side effects, reduced bioavailability, high cost and chemoresistance, amongst other problems, limit the use of available drugs. In recent years, natural compounds have shown promise as anti-leishmanial agents, especially those extracted from medicinal plants. The genus Piper has been used in traditional medicine and widely explored for its biological properties and bioactive phytocompounds. Aim: The aim of this study was to conduct a systematic literature review of the biological activity of the genus Piper against the etiological agents of leishmaniasis, to provide a perspective for effective and safe phytotherapics, new drugs or potentially active prototype chemical substances. Methodology: This systematic review was prepared in accordance with PRISMA guidelines. The databases used for this review were SciELO, Pubmed, ScienceDirect and Google Scholar, using a temporal profile of 2009 to 2020. Conclusion: In this review, we summarize a wide range of isolated compounds, extracts and essential oils of the genus Piper that are worth screening given their potential for development as effective anti-leishmanial drugs.

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“…fasciculata (Solanaceae) have promising activity against Leishmania amazonensis [ 11 ]; (ii) extracts from leaves and stem bark of Crescentia cujete (Binoniacea) have anti-inflammatory and antibacterial in vitro activities; possibly related to tannins, flavonoids, flavones and flavonols [ 12 ]; (iii) extracts from Clusia (Clusiaceae) species and Malpighia glabra (Malpighiaceae) rich in carotenoids and flavonoids present potent antioxidant activity [ 13 , 14 , 15 ]; and (iv) aqueous extract pf Rheedia longifolia (Clusiaceae) leaves has analgesic and anti-inflammatory in vivo activities and low toxicity [ 16 ]. Additionally, natural products from Piper species (Piperaceae) display broad biological roles [ 17 , 18 , 19 , 20 , 21 ], including antiprotozoal effects [ 22 ]. Additionally, a chalcone isolated from inflorescences of P. aduncum [ 23 ] and an essential oil and the ethanolic extract from leaves of P. marginatum [ 24 ] also present promising in vitro effect against L. amazonensis .…”

Section: Introductionmentioning

confidence: 99%

In Vitro Phenotypic Activity and In Silico Analysis of Natural Products from Brazilian Biodiversity on Trypanosoma cruzi

et al. 2021

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Chagas disease (CD) affects more than 6 million people worldwide. The available treatment is far from ideal, creating a demand for new alternative therapies. Botanical diversity provides a wide range of novel potential therapeutic scaffolds. Presently, our aim was to evaluate the mammalian host toxicity and anti-Trypanosoma cruzi activity of botanic natural products including extracts, fractions and purified compounds obtained from Brazilian flora. In this study, 36 samples of extracts and fractions and eight pure compounds obtained from seven plant species were evaluated. The fraction dichloromethane from Aureliana fasciculata var. fasciculata (AFfPD) and the crude extract of Piper tectoniifolium (PTFrE) showed promising trypanosomicidal activity. AFfPD and PTFrE presented EC50 values 10.7 ± 2.8 μg/mL and 12.85 ± 1.52 μg/mL against intracellular forms (Tulahuen strain), respectively. Additionally, both were active upon bloodstream trypomastigotes (Y strain), exhibiting EC50 2.2 ± 1.0 μg/mL and 38.8 ± 2.1 μg/mL for AFfPD and PTFrE, respectively. Importantly, AFfPD is about five-fold more potent than Benznidazole (Bz), the reference drug for CD, also reaching lower EC90 value (7.92 ± 2.2 μg/mL) as compared to Bz (23.3 ± 0.6 μg/mL). Besides, anti-parasitic effect of eight purified botanic substances was also investigated. Aurelianolide A and B (compounds 1 and 2) from A. fasciculata and compound 8 from P. tuberculatum displayed the best trypanosomicidal effect. Compounds 1, 2 and 8 showed EC50 of 4.6 ± 1.3 μM, 1.6 ± 0.4 μM and 8.1 ± 0.9 μM, respectively against intracellular forms. In addition, in silico analysis of these three biomolecules was performed to predict parameters of absorption, distribution, metabolism and excretion. The studied compounds presented similar ADMET profile as Bz, without presenting mutagenicity and hepatotoxicity aspects as predicted for Bz. Our findings indicate that these natural products have promising anti-T. cruzi effect and may represent new scaffolds for future lead optimization.

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Metabolomic approach of the antiprotozoal activity of medicinal Piper species used in Peruvian Amazon

Cited by 11 publications

References 61 publications

Anti-trypanosomal screening of Salvadoran flora

Anti-trypanosomal screening of Salvadoran flora

Antileishmanial Activity of the Genus Piper: A Systematic Review

In Vitro Phenotypic Activity and In Silico Analysis of Natural Products from Brazilian Biodiversity on Trypanosoma cruzi

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