Metabolomic and proteomic characterization of sng and pain phenotypes in fibromyalgia

Abstract

Background: Fibromyalgia (FM) is characterized by chronic widespread pain.Its pathophysiological mechanisms remain poorly understood, and effective diagnosis and treatments are lacking. This study aimed to identify significantly changed biosignatures in FM and propose a novel classification for FM based on pain and soreness (sng) symptoms.Methods: Urine and serum samples from 30 FM patients and 25 controls underwent metabolomic and proteomic profiling.Results: Compared with controls, FM patients showed significant differential expression of three metabolites in urine and five metabolites and eight proteins in serum. Of them, DETP, 4-guanidinobutanoic acid, SM(d18:1/18:0), PC(20:1(11Z)/18:0), S100A7, SERPINB3, galectin-7 and LYVE1 were first reported as potential biomarkers for FM. Furthermore, lactate, 2-methylmaleate and cotinine in urine and lactate, SM(d18:1/25:1), SM(d18:1/26:1) and prostaglandin D2 (PGD2) and PCYOX1, ITIH4, PFN1, LRG1, C8G, C8A, CP, CDH5 and DBH in serum could differentiate pain-(PG) and sng-dominant groups (SG). Lactate, 2-methylmaleate, cotinine, PCYOX1, ITIH4, PFN1 and DBH have a higher level in SG. SM(d18:1/25:1), SM(d18:1/26:1), PGD2, LRG1, C8G, C8A, CP and CDH5 in SG are lower than PG. The omics results indicated disordered free radical scavenging,This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Longitudinal Change of Serum Inter-<i>α</i>-Trypsin Inhibitor Heavy Chain H4 and its Relation with Inflammation, Disease Recurrence, and Mortality in Acute Ischemic Stroke Patients

“…Inter-α-trypsin inhibitor heavy chain H4 (ITIH4), a member of the serine protease inhibitor family, is a liver-originated plasma glycoprotein (Ma et al 2021;Zhuo and Kimata 2008). It has been reported that ITIH4 modulates atherosclerosis and lipids, which are involved in the development of AIS (Hao et al 2022;Hsu et al 2022;Malaud et al 2012). Importantly, ITIH4 has the capability of regulating inflammation, the latter is detrimental to the severity and functional outcomes of AIS (Huang 2022;Ma et al 2021;Shi et al 2019).…”

Serum leucine-rich alpha-2 glycoprotein levels in rheumatoid arthritis and spondyloarthritis: A promising biomarker

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AKAP12 and RNF11 as Diagnostic Markers of Fibromyalgia and Their Correlation with Immune Infiltration

“…However, the mechanism used by RNF11 to regulate protein ubiquitination remains unclear because the RNF11 promotes ubiquitination in some cases but inhibits substrate ubiquitination in other instances [ 31 ]. Hsu et al conducted a metabolomic and proteomic analysis of serum samples from patients with FM, and the results showed that FM patients had metabolic disorders and produced numerous NF- κ B-dependent cytokines [ 32 ]. RNF11 has been reported as a negative regulator of NF- κ B and Jun N-terminal kinase (JNK) signaling pathways [ 33 ].…”