Metabolomic and elemental profiling of blood serum in bladder cancer

Ossoliński, Krzysztof; Ruman, Tomasz; Copié, Valérie; Tripet, Brian; Nogueira, Leonardo Brandão; Nogueira, Katiane O.P.C.; Kołodziej, Artur; Płaza‐Altamer, Aneta; Ossolińska, Anna; Ossoliński, Tadeusz; Nizioł, Joanna

doi:10.1016/j.jpha.2022.08.004

Cited by 23 publications

(13 citation statements)

References 93 publications

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“…Tryptophan metabolism has been extensively studied in relation to bladder cancer, and its significance has been consistently reported in the literature 60 . Previous studies have observed significant increases in tryptophan levels in urine, serum, and tissue samples from bladder cancer patients compared to control groups 18 , 61 – 63 . Sex-related differences have been identified in the metabolism of tryptophan, suggesting a potential link between sex hormones and tryptophan-related pathways in the context of bladder cancer 32 , 61 .…”

Section: Discussionmentioning

confidence: 86%

Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry

Nizioł

Ossoliński

Płaza‐Altamer

et al. 2023

Sci Rep

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Bladder cancer (BC) is a common urological malignancy with a high probability of death and recurrence. Cystoscopy is used as a routine examination for diagnosis and following patient monitoring for recurrence. Repeated costly and intrusive treatments may discourage patients from having frequent follow-up screenings. Hence, exploring novel non-invasive ways to help identify recurrent and/or primary BC is critical. In this work, 200 human urine samples were profiled using ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS) to uncover molecular markers differentiating BC from non-cancer controls (NCs). Univariate and multivariate statistical analyses with external validation identified metabolites that distinguish BC patients from NCs disease. More detailed divisions for the stage, grade, age, and gender are also discussed. Findings indicate that monitoring urine metabolites may provide a non-invasive and more straightforward diagnostic method for identifying BC and treating recurrent diseases.

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Section: Discussionmentioning

confidence: 86%

Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry

Nizioł

Ossoliński

Płaza‐Altamer

et al. 2023

Sci Rep

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“…The cancerogenesis process occurring in the brain may influence the response of the immune system also in other parts of the body and it may manifest itself with the changes in the elemental composition of tissues 36 – 38 . The elemental anomalies of various tissues and body organs associated with the occurrence of neoplastic processes are still at the stage of discovery and characterization 29 – 33 . Therefore, the aim of this investigation was identification of element abnormalities that appear in far distant organs as a result of implantation of human GBM cells into rat brain and/or subsequent tumor development.…”

Section: Discussionmentioning

confidence: 99%

“…Stages and mechanisms of aggressive expansion of neoplastic cells into their surroundings, as well as genetic indicators of the cancerogenesis itself are known to medicine. However, besides the process of metastasis, the influence of GBM on other than brain vital organs is still a matter of speculation and the information on the elemental abnormalities occurring in the distant organs may shed some new light on this problem 29 – 33 . Therefore, the purposes of our study was determination of the changes in the concentrations of major, minor and trace elements which occur in kidney, heart, spleen and lung in rats subjected to implantation of human GBM cells to the brain.…”

Section: Introductionmentioning

confidence: 99%

Glioblastoma multiforme influence on the elemental homeostasis of the distant organs: the results of inter-comparison study carried out with TXRF method

Wilk,

Setkowicz,

Banas

et al. 2024

Sci Rep

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Glioblastoma (GBM) is a fast-growing and aggressive brain tumor which invades the nearby brain tissue but generally does not spread to the distant organs. Nonetheless, if untreated, GBM can result in patient death in time even less than few months from the diagnosis. The influence of the tumor progress on organs other than brain is obvious but still not well described. Therefore, we examined the elemental abnormalities appearing in selected body organs (kidney, heart, spleen, lung) in two rat models of GBM. The animals used for the study were subjected to the implantation of human GBM cell lines (U87MG and T98G) characterized by different levels of invasiveness. The elemental analysis of digested organ samples was carried out using the total reflection X-ray fluorescence (TXRF) method, independently, in three European laboratories utilizing various commercially available TXRF spectrometers. The comparison of the data obtained for animals subjected to T98G and U87MG cells implantation showed a number of elemental anomalies in the examined organs. What is more, the abnormalities were found for rats even if neoplastic tumor did not develop in their brains. The most of alterations for both experimental groups were noted in the spleen and lungs, with the direction of the found element changes in these organs being the opposite. The observed disorders of element homeostasis may result from many processes occurring in the animal body as a result of implantation of cancer cells or the development of GBM, including inflammation, anemia of chronic disease or changes in iron metabolism. Tumor induced changes in organ elemental composition detected in cooperating laboratories were usually in a good agreement. In case of elements with higher atomic numbers (Fe, Cu, Zn and Se), 88% of the results were classified as fully compliant. Some discrepancies between the laboratories were found for lighter elements (P, S, K and Ca). However, also in this case, the obtained results fulfilled the requirements of full (the results from three laboratories were in agreement) or partial agreement (the results from two laboratories were in agreement).

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“…Ruman’s group, for urine [ 50 ] and blood serum [ 51 ] metabolite analyses, utilized the developed SALDI targets coated with monoisotopic silver ( 109 Ag) and gold (Au) nanoparticles generated through the laser ablation synthesis in solution process. Statistical analysis of the MS data enabled the complete separation of the study group with diagnosed bladder cancer from healthy volunteers, as well as the selection of m / z values that most differentiated both groups for further analysis.…”

Section: Bladder Cancermentioning

confidence: 99%

Matrix- and Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Methods for Urological Cancer Biomarker Discovery—Metabolomics and Lipidomics Approaches

Arendowski

2024

Metabolites

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Urinary tract cancers, including those of the bladder, the kidneys, and the prostate, represent over 12% of all cancers, with significant global incidence and mortality rates. The continuous challenge that these cancers present necessitates the development of innovative diagnostic and prognostic methods, such as identifying specific biomarkers indicative of cancer. Biomarkers, which can be genes, proteins, metabolites, or lipids, are vital for various clinical purposes including early detection and prognosis. Mass spectrometry (MS), particularly soft ionization techniques such as electrospray ionization (ESI) and laser desorption/ionization (LDI), has emerged as a key tool in metabolic profiling for biomarker discovery, due to its high resolution, sensitivity, and ability to analyze complex biological samples. Among the LDI techniques, matrix-assisted laser desorption/ionization (MALDI) and surface-assisted laser desorption/ionization (SALDI) should be mentioned. While MALDI methodology, which uses organic compounds as matrices, is effective for larger molecules, SALDI, based on the various types of nanoparticles and nanostructures, is preferred for smaller metabolites and lipids due to its reduced spectral interference. This study highlights the application of LDI techniques, along with mass spectrometry imaging (MSI), in identifying potential metabolic and lipid biomarkers for urological cancers, focusing on the most common bladder, kidney, and prostate cancers.

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Metabolomic and elemental profiling of blood serum in bladder cancer

Cited by 23 publications

References 93 publications

Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry

Untargeted urinary metabolomics for bladder cancer biomarker screening with ultrahigh-resolution mass spectrometry

Glioblastoma multiforme influence on the elemental homeostasis of the distant organs: the results of inter-comparison study carried out with TXRF method

Matrix- and Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Methods for Urological Cancer Biomarker Discovery—Metabolomics and Lipidomics Approaches

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