Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid

Abstract

This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution 1H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo.

Effects of Lonicera japonica extract on performance, blood biomarkers of inflammation and oxidative stress during perinatal period in dairy cows

“…In the present study, supplementation of LJE at doses of 1 and 2 g/kg DM reduced both NEFA and BHBA concentrations in blood. Previous study has demonstrated that supplemen tation with chlorogenic acid regulated energy metabolism and decreased serum BHBA concentration in rats [22]. More over, LJE supplementation increased the intake of the cows in the current study, therefore reduced the concentrations of NEFA and BHBA by alleviating the mobilization of adipose tissue.…”

Comparison study of three compounds in Eucommia ulmoides on growth, flesh quality of grass carp ( Ctenopharyngodon idella )

“…EU has been reported to promote the generation of Ser, Glu and Lys in the muscle of chickens (Wang et al, ). CGA was confirmed to increase serum Gly level and liver glutathione level of rats (Ruan et al, ) and increase the TEAA and TAA levels in grass carp (Sun et al). The present study showed that the supplementation of CGA, GP and active compound mixture significantly increased TAA in muscle based on dry matter ( p < 0.05).…”

Dietary Glutamate Supplementation Ameliorates Mycotoxin-Induced Abnormalities in the Intestinal Structure and Expression of Amino Acid Transporters in Young Pigs

“…SOD and GSH-Px are two major antioxidant enzymes to scavenge the excessive internal reactive oxygen species (ROS) which exert radical-mediates damages to biological macromolecules (proteins, lipids and DNA) [42] , therefore the activities of SOD and GSH-Px are plausibly regarded as a mark reflecting redox of organism [15] . In this study, compared with the control group, mold-contaminated feed significantly decreased T-SOD activity (P<0.05) and GSH-Px activity (P>0.05) ( Figure 2 ).…”

“…Twenty-seven amino acids were detected in serum based on our previous study [42] – [43] via LC-MS/MS (HPLC Ultimate3000 and 3200 Q TRAP LC-MS/MS): L-arginine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-phenylalanine, L-methionine, L-threonine, L-tryptophan, L-valine, glycine, L-serine, L-tyrosine, L-asparagine, L-aspartic acid, L-citrulline, L-glutamic acid, L-glutamine, L-ornithine, L-cystine, L-homocystine, L-alanine, L-carnosine, hydroxy-L-proline, 1-methyl-L-histidine, 3-methyl-L-histidine, and L-proline.…”