Metabolomic Analysis of Akt1-Mediated Muscle Hypertrophy in Models of Diet-Induced Obesity and Age-Related Fat Accumulation

Cheng, Kian Kai; Akasaki, Yuichi; Lecommandeur, Emmanuelle; Lindsay, Ross T; Murfitt, Steven A.; Walsh, Kenneth; Griffin, Julian L.

doi:10.1021/pr500756u

Cited by 17 publications

(57 citation statements)

References 32 publications

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“…Another target gene, DOCK3, that overexpressed it could regulate PTEN/AKT signaling, thereby regulating muscle hypertrophy and growth and inducing apoptosis (Alexander et al, 2014). chi-miR-324-3p, another key miRNA of profile 16, targeted AKT1, which promoted type IIb muscle growth (Cheng et al, 2015). And AKT1 also promoted load-induced muscle hypertrophy by regulating satellite cell proliferation (Moriya and Miyazaki, 2018).…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

et al. 2020

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MicroRNAs (miRNAs) are indispensable for the regulation of skeletal muscle. We performed RNA sequencing (RNA-seq) to establish a comprehensive miRNA profiling of goats in seven stages, namely, 45-(F45), 65-(F65), 90-(F90), 120-(F120), and 135-day (F135) fetuses, newborn (B1), and 90-day-old (B90) kids. In total, 421 known miRNAs and 228 goat novel miRNAs were identified in the data, and the average abundance of 19 miRNAs in seven stages exceeds 10,000 reads per million. Furthermore, 420 differentially expressed miRNAs (DEmiRNAs) were identified in all comparison group at seven stages, 80 of which were uniquely differentially expressed in the B1 and B90 comparison groups. Pathway analysis indicated that this group was associated with the release of muscle hypertrophy and regulation of myoblast proliferation. Besides, 305 DEmiRNAs were clustered into three significantly enriched profiles (profiles 11, 16, and 19). Function analysis revealed that profile 16 was related to muscle hypertrophy and differentiation. Profile 11 was involved in multiple enzyme activities and metabolic processes in muscle cells. And profile 19 was involved in material transport and structural stability. Two highly expressed miRNAs and three key miRNAs (chi-miR-328-3p, chi-miR-767, and chi-miR-150) of these profiles were verified to be consistent with the data by quantitative real-time PCR. These results provided a catalog of goat muscleassociated miRNAs, allowing us to better understand the transformation of miRNA roles during mammalian muscle development.

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Section: Discussionmentioning

confidence: 99%

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

et al. 2020

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“…These data suggest that APP feeding might convert the lipid composition of fast fibers to that of slow fibers. The accumulation of DHA-containing PC has positive effects on the prevention of obesity 12 , and the amount of PC 18:0/22:6 was increased in a chronic training-induced model of muscle hypertrophy 4 . Therefore, the increase of PC 18:0/22:6 due to APP feeding suggested that APP has effects on lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

et al. 2019

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Alaska pollack protein (APP) was previously shown to reduce serum triacylglycerol and the atherogenic index and significantly increase gastrocnemius muscle mass in rats. To determine which myofibers are involved in this observed hypertrophy, we stained the gastrocnemius muscle with fast and slow fiber-specific antibodies and measured the muscle fiber diameter. We observed muscle hypertrophy in both the fast and slow fibers of APP-fed rats. Although muscle hypertrophy leads to drastic lipid changes, the amount of lipids did not differ significantly between casein-fed and APP-fed rats. To determine the lipid changes at the molecular species level and their localization, we performed matrix-assisted laser desorption/ ionization mass spectrometry imaging to visualize lipids in the gastrocnemius muscles. We determined that lipid molecules were significantly changed due to APP feeding. Thus, APP feeding changes muscle lipid metabolism, and these metabolic changes might be related to hypertrophy.

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“…AKT1, a serine threonine protein kinase, is a critical mediator of cell growth and survival. AKT1 transgene activation promotes type IIb fiber hypertrophy and increases glycolysis while reducing fat accumulation 35 , 36 . The up-regulation of AKT1 in PiNN may have a functional link to its leanness.…”

Section: Discussionmentioning

confidence: 99%

Muscle Transcriptional Profile Based on Muscle Fiber, Mitochondrial Respiratory Activity, and Metabolic Enzymes

Li¹,

Du²,

Trakooljul³

et al. 2015

Int. J. Biol. Sci.

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Skeletal muscle is a highly metabolically active tissue that both stores and consumes energy. Important biological pathways that affect energy metabolism and metabolic fiber type in muscle cells may be identified through transcriptomic profiling of the muscle, especially ante mortem. Here, gene expression was investigated in malignant hyperthermia syndrome (MHS)-negative Duroc and Pietrian (PiNN) pigs significantly differing for the muscle fiber types slow-twitch-oxidative fiber (STO) and fast-twitch-oxidative fiber (FTO) as well as mitochondrial activity (succinate-dependent state 3 respiration rate). Longissimus muscle samples were obtained 24 h before slaughter and profiled using cDNA microarrays. Differential gene expression between Duroc and PiNN muscle samples were associated with protein ubiquitination, stem cell pluripotency, amyloid processing, and 3-phosphoinositide biosynthesis and degradation pathways. In addition, weighted gene co-expression network analysis within both breeds identified several co-expression modules that were associated with the proportion of different fiber types, mitochondrial respiratory activity, and ATP metabolism. In particular, Duroc results revealed strong correlations between mitochondrion-associated co-expression modules and STO (r = 0.78), fast-twitch glycolytic fiber (r = -0.98), complex I (r=0.72) and COX activity (r = 0.86). Other pathways in the protein-kinase-activity enriched module were positively correlated with STO (r=0.93), while negatively correlated with FTO (r = -0.72). In contrast to PiNN, co-expression modules enriched in macromolecule catabolic process, actin cytoskeleton, and transcription activator activity were associated with fiber types, mitochondrial respiratory activity, and metabolic enzyme activities. Our results highlight the importance of mitochondria for the oxidative capacity of porcine muscle and for breed-dependent molecular pathways in muscle cell fibers.

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Metabolomic Analysis of Akt1-Mediated Muscle Hypertrophy in Models of Diet-Induced Obesity and Age-Related Fat Accumulation

Cited by 17 publications

References 32 publications

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

RNA-Seq Reveals miRNA Role Shifts in Seven Stages of Skeletal Muscles in Goat Fetuses and Kids

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

Muscle Transcriptional Profile Based on Muscle Fiber, Mitochondrial Respiratory Activity, and Metabolic Enzymes

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