Metabolomic analysis applied to chemosystematics and evolution of megadiverse Brazilian Vernonieae (Asteraceae)

Gallon, Marília Elias; Monge, Marcelo; Casoti, Rosana; Costa, Fernando B. Da; Semir, João; Gobbo‐Neto, Leonardo

doi:10.1016/j.phytochem.2018.03.007

Cited by 18 publications

(18 citation statements)

References 36 publications

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“…Several chemotaxonomic studies have applied the differential incidence of their skeleton subtype to deduce taxonomic relationships [30,31,32]. For instance, Vernonieae species are characterized by the presence of glaucolides, hirsutinolides, furanoheliangolides, and eremantholides; the Eupatorieae tribe is characterized by guaianolides, heliangolides, and mikanolides; and, the Heliantheae tribe, which comprises T. diversifolia , is characterized by eudesmanolides, furanoheliangolides, heliangolides, and melampolides [33,34]. More than their chemotaxonomic significance, STL play important roles in plant protection against herbivores and microorganisms, as well as in plant growth inhibition [35].…”

Section: Resultsmentioning

confidence: 99%

Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)

et al. 2019

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The chemical ecology of plant-insect interactions has been driving our understanding of ecosystem evolution into a more comprehensive context. Chlosyne lacinia (Lepidoptera: Nymphalidae) is an olygophagous insect herbivore, which mainly uses host plants of Heliantheae tribe (Asteraceae). Herein, plant-insect interaction between Tithonia diversifolia (Heliantheae) and Chlosyne lacinia was investigated by means of untargeted LC-MS/MS based metabolomics and molecular networking, which aims to explore its inherent chemical diversity. C. lacinia larvae that were fed with T. diversifolia leaves developed until fifth instar and completed metamorphosis to the adult phase. Sesquiterpene lactones (STL), flavonoids, and lipid derivatives were putatively annotated in T. diversifolia (leaves and non-consumed abaxial surface) and C. lacinia (feces, larvae, pupae, butterflies, and eggs) samples. We found that several furanoheliangolide-type STL that were detected in T. diversifolia were ingested and excreted in their intact form by C. lacinia larvae. Hence, C. lacinia caterpillars may have, over the years, developed tolerance mechanisms for STL throughout effective barriers in their digestive canal. Flavonoid aglycones were mainly found in T. diversifolia samples, while their glycosides were mostly detected in C. lacinia feces, which indicated that the main mechanism for excreting the consumed flavonoids was through their glycosylation. Moreover, lysophospholipids were predominately found in C. lacinia samples, which suggested that they were essential metabolites during pupal and adult stages. These findings provide insights into the natural products diversity of this plant-insect interaction and contribute to uncovering its ecological roles.

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Section: Resultsmentioning

confidence: 99%

Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)

et al. 2019

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“…Asteraceae is the largest family which has been divided into 12 sub-families [44], [45]. This group member synthesizes the terpenoids, flavonoids, and polyacetylenes, while the sesquiterpene lactones are the compound group used as its taxonomic markers [46]. Asteroidea, known to be the largest subfamily in Asteraceaewhich is comprised of approximately 15,500 species belong to 1,229 genera and 20 tribes (represent 60-70% in the family) [36], [47].…”

Section: Resultsmentioning

confidence: 99%

Using Phylogeny Approach on Ethnobotanical Bioprospecting for Leading Antimalarial Plant-Based Drug Discovery

Liana¹,

Rungsihirunrat²

2021

Int. J. Adv. Sci. Eng. Inf. Technol.

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Ethnobotanical-directed bioprospecting has made a significant contribution to modern drug discoveries. However, merely relying on this approach may spend more expenditure, time-consuming, and lead exhaustive laboratory testing due to the tremendous data of medicinal plants used and the occurrence of placebo effect during traditional medical treatment. Combining the phylogeny approach with ethnobotanical bioprospecting may become new prospective tools to lead the plant-based drug discovery, including antimalaria. This study aimed to map the ethnomedicinal plants used by various indigenous cultures to investigate the clustered pattern of its antimalarial properties for future bioprospecting. The Internal Transcribed Spacer (ITS) region sequences of selected 280 medicinal plants taxa obtained from NCBI (National Center for Biotechnology Information) were aligned by MUSCLE multiple sequences alignments. They were further analyzed using the Maximum Likelihood Phylogenetic Test by MEGA X software to construct the phylogenetic tree. Our research revealed that the medicinal plant taxa for malaria treatment was clumped in several families, including Apocynaceae, Euphorbiaceae, Rubiaceae, Rutaceae, Fabaceae were strongly clumped along with plants used for fever in the Asteraceae family. Interestingly, our finding showed that these plants were clumped in the sub-family of antimalarial producing species, the Asteroidea. Furthermore, the strongly clumping pattern was also shown in the tribe Heliantheae alliance of this sub-family. This finding supports the predictive power of phylogeny for future bioprospecting to select the candidate taxa to lead the drug discovery.

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“…Metabolomics have been applied in many different research areas, such as diagnostics 19 , chemosystematics 20 , dereplication 21 , among others 22 . In recent work, Graziani and co-workers adopted a metabolomics approach to www.nature.com/scientificreports www.nature.com/scientificreports/ identify cytotoxic agents from Fabaceae species 23 .…”

Section: Discussionmentioning

confidence: 99%

Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products

Demarque

Dusi

Sousa

et al. 2020

Sci Rep

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Metabolomics is a powerful tool in the analysis and identification of metabolites responsible for biological properties. Regarding natural product chemistry, it constitutes a potential strategy to streamline the classic and laborious process of isolating natural products, which often involves the re-isolation and identification of known compounds. In this contribution, we establish a mass spectrometry-based metabolomics strategy to discover compounds with larvicidal activity against Aedes aegypti. We analyse the Brazilian plant Annona crassiflora using different platforms to annotate the active compounds in different extracts/fractions of various plant parts. The MetaboAnalyst and GNPS platforms, which consider LC-MS and LC-MS/MS data, respectively, were chosen to identify compounds that differentiate active and inactive samples. Bio-guided isolation was subsequently performed to confirm compound activity. Results proved the capacity of metabolomics to predict metabolite differences between active and inactive samples using LC-MS and LC-MS/MS data. Moreover, we discuss the limitations, possibilities, and strategies to have a broad view of vast data.

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Metabolomic analysis applied to chemosystematics and evolution of megadiverse Brazilian Vernonieae (Asteraceae)

Cited by 18 publications

References 36 publications

Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)

Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)

Using Phylogeny Approach on Ethnobotanical Bioprospecting for Leading Antimalarial Plant-Based Drug Discovery

Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products

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