Metabolomic analyses of the bio-corona formed on TiO2 nanoparticles incubated with plant leaf tissues

Kurepa, Jasmina; Shull, Timothy E.; Smalle, Jan

doi:10.1186/s12951-020-00592-8

Cited by 27 publications

(21 citation statements)

References 64 publications

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“…We found that the bio-coronas of anatase TiO 2 NP incubated with Tulsi leaves and madder roots are rich in flavonoids, lipids, and peptides. Together with the conclusions of our prior study [ 8 ], we show that nanoharvesting using anatase TiO 2 NP from any plant source can be used to enrich flavonoid and lipid compounds, which can then be used in targeted bio-assays.…”

Section: Introductionsupporting

confidence: 73%

“…A pool of three young leaves excised from separate plants or a mix of root segments from different plants (~ 100 mg tissue per sample) was used for both the methanolic extraction and nanoharvesting using a previously described method [ 8 ]. In brief, to obtain methanolic extracts, tissues were frozen in liquid nitrogen, disrupted using zirconium beads and a bead beater in 10 volumes of 1% HCl/methanol, and incubated in acid methanol for 16 h in the dark at 4 °C.…”

Section: Main Textmentioning

confidence: 99%

“…Untargeted MS analysis was performed at the Proteomics & Mass Spectrometry Facility at the Danforth Plant Science Center, as previously described [ 8 ]. For data processing, datasets were analyzed using MetaboAnalyst 3.0 ( www.metaboanalyst.ca [ 28 ]) and the R package ComplexHeatmap [ 29 ] using previously described parameters [ 8 ].…”

Section: Main Textmentioning

confidence: 99%

“…We showed that flavonoids with a catechol ring were more abundant in NP bio-coronas than flavonoids without the catechol ring [ 6 ]. Untargeted metabolomic analyses of the bio-coronas formed on the surface of TiO 2 NPs incubated with Arabidopsis tissues confirmed that flavonoids are preferred ligands but also showed that lipids and, in particular, fatty acids are avidly bound to the NP surface, thus competing with flavonoids [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum

Kurepa

Smalle

2021

BMC Res Notes

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Objective Nanoharvesting from intact plants, organs, and cultured cells is a method in which nanoparticles are co-incubated with the target tissue, which leads to the internalization of nanoparticles. Internalized nanoparticles are coated in situ with specific metabolites that form a dynamic surface layer called a bio-corona. Our previous study showed that metabolites that form the bio-corona around anatase TiO2 nanoparticles incubated with leaves of the model plant Arabidopsis thaliana are enriched for flavonoids and lipids. The present study focused on the identification of metabolites isolated by nanoharvesting from two medicinal plants, Ocimum sanctum (Tulsi) and Rubia tinctorum (common madder). Results To identify metabolites that form the bio-corona, Tulsi leaves and madder roots were incubated with ultra-small anatase TiO2 nanoparticles, the coated nanoparticles were collected, and the adsorbed molecules were released from the nanoparticle surface and analyzed using an untargeted metabolomics approach. Similar to the results in which Arabidopsis tissue was used as a source of metabolites, TiO2 nanoparticle bio-coronas from Tulsi and madder were enriched for flavonoids and lipids, suggesting that nanoharvesting has a wide-range application potential. The third group of metabolites enriched in bio-coronas isolated from both plants were small peptides with C-terminal arginine and lysine residues.

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Section: Introductionsupporting

confidence: 73%

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum

Kurepa

Smalle

2021

BMC Res Notes

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“…In the presence of biological fluids nanoparticle (NPs) are quickly coated with various biomolecules which ultimately form a biocorona [1]. The NP-biocorona is composed of proteins, lipids [2], nucleic acids [3] and metabolites [4]. The protein corona plays a pivotal role in the interaction of NPs with biological systems and has therefore been studied extensively [2,5].…”

Section: Introductionmentioning

confidence: 99%

Plasma proteins facilitates placental transfer of polystyrene particles

et al. 2020

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Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications.

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Role of root exudates on the transformation and ecological effect of engineering nanomaterials in soil system: A critical review

Wei,

Miao,

Zhou

et al. 2024

Land Degrad Dev

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Due to the widespread use of engineered nanomaterials (ENMs) for soil remediation and nano‐enabled sustainable agriculture, there is a growing concern regarding the behavior and fate of ENMs released into soil systems in the presence of natural root exudates (REs). Herein, we investigate the influence of REs on the fate and ecological effect of ENMs from a comprehensive perspective. We summarize the key roles reported in the literature for REs in physical changes (e.g., adsorption, dispersion/aggregation), chemical changes (e.g., oxidation/redox reactions, and dissolution), and biotransformation of ENMs, which will further determine the ecological risk of ENMs in natural soil systems. Moreover, this review highlights the potential adverse effects of ENMs on different soil organisms (e.g., bacteria, plants, and eisenia foetida) in the presence of REs. The remaining unclear mechanisms (e.g., oxidative stress and DNA damage) of ENMs toxicity at the cellular level influenced by REs are reviewed and presented. Finally, the review concludes by addressing the current knowledge gaps and challenges in this field.

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Metabolomic analyses of the bio-corona formed on TiO2 nanoparticles incubated with plant leaf tissues

Cited by 27 publications

References 64 publications

Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum

Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum

Plasma proteins facilitates placental transfer of polystyrene particles

Role of root exudates on the transformation and ecological effect of engineering nanomaterials in soil system: A critical review

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