Metabolomic Analyses of Blood Plasma after Oral Administration of D-Glucosamine Hydrochloride to Dogs

Osaki, Tomohiro; Aso, Kazuo; Kurozumi, Seizi; Takamori, Yoshimori; Tsuka, Takeshi; Imagawa, Tomohiro; Okamoto, Yoshiharu; Minami, Saburo

doi:10.3390/md10081873

Cited by 13 publications

(9 citation statements)

References 32 publications

(36 reference statements)

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“…Maximam plasma GlcN concentrations are reached at 0.5 hr after oral administration of GlcN [24]. Absorbed GlcN influences the metabolism of amino acids associated with collagen synthesis [25]. In contrast, fucoidan is a polymer composed of fucose, and an increase in plasma fucose was not observed in this study (data not shown).…”

Section: Discussionmentioning

confidence: 74%

Effect of Fucoidan Extracted from Mozuku on Experimental Cartilaginous Tissue Injury

et al. 2012

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We investigated the effect of fucoidan, a sulfated polysaccharide, on acceleration of healing of experimental cartilage injury in a rabbit model. An injured cartilage model was surgically created by introduction of three holes, one in the articular cartilage of the medial trochlea and two in the trochlear sulcus of the distal femur. Rabbits in three experimental groups (F groups) were orally administered fucoidan of seven different molecular weights (8, 50, 146, 239, 330, 400, or 1000 kD) for 3 weeks by screening. Control (C group) rabbits were provided water ad libitum. After the experimental period, macroscopic examination showed that the degree of filling in the fucoidan group was higher than that in the C group. Histologically, the holes were filled by collagen fiber and fibroblasts in the C group, and by chondroblasts and fibroblasts in the F groups. Image analysis of Alcian blue- and safranin O-stained F-group specimens showed increased production of glycosaminoglycans (GAGs) and proteoglycans (PGs), respectively. Some injured holes were well repaired both macroscopically and microscopically and were filled with cartilage tissues; cartilage matrices such as PGs and GAGs were produced in groups F 50, F 146, and F 239. Thus, fucoidan administration enhanced morphologically healing of cartilage injury.

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Section: Discussionmentioning

confidence: 74%

Effect of Fucoidan Extracted from Mozuku on Experimental Cartilaginous Tissue Injury

et al. 2012

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“…An increase in organic acids resulting from administration of GlcN was previously reported by Osaki et al 45 Lactic acid could, in principle, be imaged with CEST MRI by applying selective saturation at 0.4 ppm, corresponding to the MR frequency of lactate's hydroxyl group 46 . But, due to the small frequency gap between the water and lactate peaks, this contribution would not be detected from the total GlcN CEST effect.…”

Section: Discussionmentioning

confidence: 85%

Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study

Rivlin

2020

NMR in Biomedicine

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Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique. Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation. Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors. Significant CEST effects were observed at around 1.5, 3.6 and −3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively. The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C]-GlcNÁHCl. The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal. The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN. The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.

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“…In the past ten years, extensive awareness was created on the potential therapeutic use of cytidine and β-D-N 4 -hydroxycytidine analogues acting on diverse biological targets for several cancers and viral infections. Until 2015, majority of the cytidine scaffolds were developed to accomplish the potential activities that mimic micro RNA (miRNA), [30] block murine norovirus (MNV) replication, [31] influence fumarate respiration and starvation in cells, [32] Hepatitis B virus (HBV) infected human hepatocytes, [33] catalytic DNA methyltransferase inhibitor, [34] CMP-pseudaminic acid synthesis in Bacillus thuringiensis, [35] etc. Other biological targets of cytidine analogues disclosed after 2015 include, acetyltransferase NAT10, [36] β-D-galactoside sialyltransferases, [37] HBV infection, [38] and NLRP3 inflammasome activation.…”

Section: Recent Developments Of Therapeutic Cytidine Derivativesmentioning

confidence: 99%

Therapeutic Inhibitory Activities of N‐Hydroxy Derived Cytidines: A Patent Overview

et al. 2021

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The structural enantiomers of nucleoside analogs are cytidine and β‐D‐N4‐hydroxycytidine derivatives. This implies their significance as excellent anticancer agents by their compatibility with DNA/RNA polymerases and antiviral agents in particular, as polymerase inhibitors by with their ability to selectively inhibit against the Human Corona Virus and Severe acute respiratory syndrome coronavirus (SARS‐CoV) by reduction assay. This encouraged us to explore and collect the available data on the subject of therapeutic significance of cytidines and β‐D‐N4‐hydroxycytidines. Many of their pharmacologically significant derivatives can serve as alternate targets to be investigated for various therapeutic applications. However, only a few cytidine and β‐D‐N4‐hydroxycytidine derivatives were patented in the past decade. The Half maximal inhibitory concentration (IC50) values of these patented therapeutic inhibitors range between 0.003 to<50 μM, depending on the nature of the biological assays. The current review examines cytidine and β‐D‐N4‐hydroxycytidine derivatives as anticancer and antiviral targets, to explore over their developments with reference to the advancement of biological, pharmacological and therapeutic activates reported in patents over the last decade. Using various databases of Justia patent, Lens database and Google patents, the granted patents from 2010 to 2020 were retrieved. It is revealed that, the discovery of cytidine scaffolds were encouraged and disclosed for further improvements as therapeutical targets. Further the increase in the number of scientific reports and publications covering anticancer and antiviral agents for selective treatment of different cancers and viral infections indicate the significance of this subject along with few limitations that seek investigations in terms of optimistic, and interactive biological effects.

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Metabolomic Analyses of Blood Plasma after Oral Administration of D-Glucosamine Hydrochloride to Dogs

Cited by 13 publications

References 32 publications

Effect of Fucoidan Extracted from Mozuku on Experimental Cartilaginous Tissue Injury

Effect of Fucoidan Extracted from Mozuku on Experimental Cartilaginous Tissue Injury

Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study

Therapeutic Inhibitory Activities of N‐Hydroxy Derived Cytidines: A Patent Overview

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