Metabolome shifts triggered by chlorine sanitisation induce Escherichia coli on fresh produce into the viable but nonculturable state

Wang, Yue; Chen, Zihui; Zhao, Feng; Yang, Hongshun

doi:10.1016/j.foodres.2023.113084

Cited by 13 publications

(5 citation statements)

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“…Additionally, it has been determined that during the VBNC state, there is an augmentation in the amino acid content, consequently leading to the alteration of specific amino acid metabolic pathways, such as the biosynthesis of aminoacyl-tRNA and the metabolism of arginine, proline, alanine, aspartate, and glutamate. Additionally, shifts were observed in D-glutamine and D-glutamate metabolism, β-alanine and arginine biosynthesis, and the valine, leucine, and isoleucine degradation pathways Another notable metabolic change was a substantial increase in cAMP content, which could potentially function as an inducing factor for the VBNC state [ 129 ]. Other studies suggest that the glyoxylate cycle serves as a pivotal metabolic pathway for stress resistance and in maintaining cellular metabolic balance during the VBNC state [ 130 ].…”

Section: Proteomics Transcriptomics and Metabolomics Of Bacterial Cel...mentioning

confidence: 99%

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The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria

Pazos-Rojas,

Cuellar-Sánchez,

Romero-Cerón

et al. 2023

Microorganisms

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Many bacteria have the ability to survive in challenging environments; however, they cannot all grow on standard culture media, a phenomenon known as the viable but non-culturable (VBNC) state. Bacteria commonly enter the VBNC state under nutrient-poor environments or under stressful conditions. This review explores the concept of the VBNC state, providing insights into the beneficial bacteria known to employ this strategy. The investigation covers different chemical and physical factors that can induce the latency state, cell features, and gene expression observed in cells in the VBNC state. The review also covers the significance and applications of beneficial bacteria, methods of evaluating bacterial viability, the ability of bacteria to persist in environments associated with higher organisms, and the factors that facilitate the return to the culturable state. Knowledge about beneficial bacteria capable of entering the VBNC state remains limited; however, beneficial bacteria in this state could face adverse environmental conditions and return to a culturable state when the conditions become suitable and continue to exert their beneficial effects. Likewise, this unique feature positions them as potential candidates for healthcare applications, such as the use of probiotic bacteria to enhance human health, applications in industrial microbiology for the production of prebiotics and functional foods, and in the beer and wine industry. Moreover, their use in formulations to increase crop yields and for bacterial bioremediation offers an alternative pathway to harness their beneficial attributes.

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Section: Proteomics Transcriptomics and Metabolomics Of Bacterial Cel...mentioning

confidence: 99%

“…Cells that left the non-culturable state had higher levels of cysteine, L-glutamic acid, L-arginine, and L-glutamine. A compound identified at high levels was xylooligosaccharide, which probably favors the return to the cultivable state [ 129 ].…”

Section: Proteomics Transcriptomics and Metabolomics Of Bacterial Cel...mentioning

confidence: 99%

The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria

Pazos-Rojas,

Cuellar-Sánchez,

Romero-Cerón

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Cells that left the nonculturable state had higher levels of cysteine, L-glutamic acid, L-arginine, and L-glutamine. A compound identified at high levels was xylooligosaccharide, which probably favors the return to the cultivable state [129].…”

Section: Proteomics Transcriptomics and Metabolomics Of Bacterial Cel...mentioning

confidence: 99%

The Viable but Non-Culturable (VBNC) State, a Poorly-Explored Aspect of Beneficial Bacteria

Pazos-Rojas,

Cuellar-Sánchez,

Romero-Cerón

et al. 2023

Preprint

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Many bacteria have the ability to survive in challenging environments; however, they cannot grow on standard culture media, a phenomenon known as the Viable but Non-Culturable (VBNC) state. Bacteria commonly go into the VBNC state under nutrient-poor environments or under stressful conditions. This review explores the concept of the VBNC state, providing insights into the beneficial bacteria known to employ this strategy. The investigation covers different chemical and physical factors that can induce the latency state, cell features, and gene expression observed in cells in the VBNC state. The revision also covered the significance and applications of beneficial bacteria, methods for evaluating bacterial viability, the ability of bacteria to persist in environments associated with higher organisms, and the factors that facilitate the return to the culturable state. Knowledge about beneficial bacteria capable of entering the VBNC state remains limited, however, beneficial bacteria in this state could face adverse environmental conditions, and return to culturable state when conditions become suitable and continue to exert their beneficial effects. Likewise, this unique feature positions them as potential candidates for healthcare applications, such as the use of probiotic bacteria to enhance human health, applications in industrial microbiology for the production of prebiotics, functional foods, and in the beer and wine industry. Moreover, their use in formulations to increase crop yield and for bacterial bioremediation offers an alternative pathway to harness their beneficial attributes.

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“…However, the recent finding states that chlorine has the potential to induce E. coli cells to enter the VBNC state, which could be a new challenge for the fresh produce sector. 19 Therefore, VBNC E. coli should be considered when testing for pathogens.…”

Section: Introductionmentioning

confidence: 99%

Progress in methods for the detection of viable Escherichia coli

Zhuang,

Gong,

Zhao

et al. 2024

Analyst

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Metabolome shifts triggered by chlorine sanitisation induce Escherichia coli on fresh produce into the viable but nonculturable state

Cited by 13 publications

References 50 publications

The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria

The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria

The Viable but Non-Culturable (VBNC) State, a Poorly-Explored Aspect of Beneficial Bacteria

Progress in methods for the detection of viable Escherichia coli

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