Metabolome‐Based Genome‐Wide Association Study of Duck Meat Leads to Novel Genetic and Biochemical Insights

Abstract

Meat is among the most consumed foods worldwide and has a unique flavor and high nutrient density in the human diet. However, the genetic and biochemical bases of meat nutrition and flavor are poorly understood. Here, 3431 metabolites and 702 volatiles in 423 skeletal muscle samples are profiled from a gradient consanguinity segregating population generated by Pekin duck × Liancheng duck crosses using metabolomic approaches. The authors identified 2862 metabolome‐based genome‐wide association studies (mGWAS) signals and 48 candidate genes potentially modulating metabolite and volatile levels, 79.2% of which are regulated by cis‐regulatory elements. The level of plasmalogen is significantly associated with TMEM189 encoding plasmanylethanolamine desaturase 1. The levels of 2‐pyrrolidone and glycerophospholipids are regulated by the gene expression of AOX1 and ACBD5, which further affects the levels of volatiles, 2‐pyrrolidone and decanal, respectively. Genetic variations in GADL1 and CARNMT2 determine the levels of 49 metabolites including L‐carnosine and anserine. This study provides novel insights into the genetic and biochemical basis of skeletal muscle metabolism and constitutes a valuable resource for the precise improvement of meat nutrition and flavor.

Tryptophan Promotes the Production of Xanthophyll Compounds in Yellow Abdominal Fat through HAAO

“…The LIT and QQQ scans were recorded on a triple quadrupole linear ion trap mass spectrometer (QTRAP), QTRAP ® LC-MS/MS system, equipped with ESI Turbo ion spray interface, operated in positive and negative ion modes. All operations and measurements were performed at the Wuhan Metware Biotechnology Co., Ltd. [ 21 ].…”

Dietary riboflavin supplementation improves meat quality, antioxidant capacity, fatty acid composition, lipidomic, volatilomic, and proteomic profiles of breast muscle in Pekin ducks

“…Thereafter, 300 μL supernatant was removed, concentrated, and stored at −80 °C. The lipidomic analysis were performed with an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC Shimadzu CBM30A system, Shimadzu, Kyoto, Japan; MS, Applied Biosystems 6500 QTRAP, Foster City, CA, USA) equipped with an ESI Turbo Ionspray interface according to the method reported previously ( Liu et al, 2023 ). Orthogonal partial least squares discriminate analysis (OPLS-DA) was done with MetaboAnalyst 5.0 ( https://www.metaboanalyst.ca/ ).…”

Research progress and applications of genome‐wide association study in farm animals

“…[113]. Liu et al drafted a fine-mapped SNPmetabolites profile in ducks and showed that GADL1 and CARNMT2 were prominent factors affecting 49 muscular metabolites [112]. In cattle, Li et al identified the candidate SNPs associated with betaine, L-Lactic acid, and L-Alanine and the range of the proportion of SNP additive variance was from 0.62% to 4.21% [121].…”

“…Liu et al. drafted a fine‐mapped SNP‐metabolites profile in ducks and showed that GADL1 and CARNMT2 were prominent factors affecting 49 muscular metabolites [112]. In cattle, Li et al.…”