Metabolome analysis for investigating host-gut microbiota interactions

Chen, Michael X.; Wang, San-Yuan; Kuo, Ching‐Hua; Tsai, I‐Lin

doi:10.1016/j.jfma.2018.09.007

Cited by 133 publications

(123 citation statements)

References 111 publications

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“…However, although representing powerful tools for bacterial identification and classification, microbiomics does not allow to obtain information about fluctuations in metabolic activities 1 . To this purpose, metabolomics is the most suitable approach, and numerous reports based on metabolomic analysis have been reported over the last decade 7 36 . Focusing on the application of metabolomics in the study of diet-microbiota interactions and searching for the keywords "metabolomics AND diet AND microbiota" in PubMed, we found that the number of publications almost doubled from to 2018, as an index of the popularity that metabolomics gained during the recent years ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

Peron

Hidalgo‐Liberona

González‐Domínguez

et al. 2019

J. Agric. Food Chem.

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The gastrointestinal tract hosts the largest microbial population of the human body, which works in symbiosis with the host to provide several important functions and contributes to the maintenance of host health. The diet is one of the factors that can most affect the gut microbiota, with subsequent consequences on host health. One consequence of changes in microbiota is changes in intestinal permeability (IP); disruption of this latter is related to the development of several diseases and is a frequent condition in older people. Nevertheless, the molecular pathways regulating these effects are still unclear, and a comprehensive understanding of the dietary components that can affect IP is lacking. Metabolomics, that has been widely used to study the transformation of nutrients by intestinal microbiota, could be a suitable approach for this purpose. However, up to now, the research has focused mainly on dietary fibers and tryptophan, while the activity of dietary polyphenols remains almost completely unexplored. Hence, the aim here was to review the most recent literature concerning the application of metabolomics in the study of the correlation between diet-induced alterations of gut microbiota and the effects on intestinal permeability, with a particular focus on the discovery of the molecular pathways involved. An additional aim was to give a perspective on the future research involving dietary polyphenols, given that despite their potential implication for the prevention and treatment of several diseases related to increased intestinal permeability, few studies have been reported to date.

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Section: Introductionmentioning

confidence: 99%

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

Peron

Hidalgo‐Liberona

González‐Domínguez

et al. 2019

J. Agric. Food Chem.

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“…Another item of note is although our top hit of N6,N6-dimethyllysine is relatively unknown in the literature, our second most significantly heterogeneous amino acid analogues, N-acetyltryptophan, demonstrated the same pattern as the BCAAs; it is also been shown to be associated with host-gut microbiota interactions in both blood and urine bio-specimens. [45–47] Namely that the amino acid tryptophan is modified into N-acetyltryptophan by the gut microbiota and then absorbed into the host human. This adds to our earlier statement that the microbiome is important to consider during exercise/behavioral obesity interventions and less so during surgical interventions.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic profiling identifies complex lipid species associated with response to weight loss interventions

Bihlmeyer

Kwee

Clish

et al. 2020

Preprint

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Obesity is an epidemic internationally. While weight loss interventions are efficacious, they are compounded by heterogeneity with regards to clinically relevant metabolic responses. Thus, we sought to identify metabolic pathways and biomarkers that distinguish individuals with obesity who would most benefit from a given type of intervention. Liquid chromatography mass spectrometry-based profiling was used to measure 765 metabolites in baseline plasma from three different weight loss studies: WLM (behavioral intervention, N=443), STRRIDE-PD (exercise trial, N=163), and CBD (surgical cohort, N=125). The primary outcome was percent change in insulin resistance (as measured by the Homeostatic Model Assessment of Insulin Resistance [%ΔHOMA-IR]) over the intervention. Overall, 92 individual metabolites were associated with %ΔHOMA-IR after adjustment for multiple comparisons. Concordantly, the most significant metabolites were triacylglycerols (TAGs; p=2.3e-5) and diacylglycerols (DAGs; p=1.6e-4), with higher TAG and DAG levels associated with a greater improvement in HOMA-IR. In tests of heterogeneity, 50 metabolites changed differently between weight loss interventions; we found amino acids, peptides, and their analogues to be most significant (4.7e-3) in this category. Our results highlight novel metabolic pathways associated with heterogeneity in response to weight loss interventions, and related biomarkers which could be used in future studies of personalized approaches to weight loss interventions.

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“…As these intestinal metabolites are presumably present in big numbers, research methods that allow comprehension of big numbers and complex metabolite patterns such as untargeted metabolomics analysis are necessary to find new drug targets to customize the treatment of CMD [72]. To identify new metabolites that influence host metabolism, various body samples can be studied such as organ tissue, blood plasma or serum, feces, urine, saliva cerebrospinal fluid, and exhaled breath [73]. Metabolites of these samples are then analyzed by nuclear magnetic resonance spectroscopy and mass spectrometry in tandem with methods such as capillary electrophoresis, liquid and gas chromatography, the latter being considered the gold standard for metabolomics analysis until so far [74].…”

Section: Microbiota-derived Metabolites Linked To Cmdmentioning

confidence: 99%

Gut microbiota: a promising target against cardiometabolic diseases

Warmbrunn

Herrema

Aron‐Wisnewsky

et al. 2020

Expert Review of Endocrinology & Metabolism

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Introduction: Cardiometabolic diseases (CMD) are a group of interrelated disorders such as metabolic syndrome, type 2 diabetes mellitus and cardiovascular diseases (CVD). As the prevalence of these diseases increases globally, efficient new strategies are necessary to target CMD and modifiable risk factors. In the past decade, evidence has accumulated regarding the influence of gut microbiota (GM) on CMD, providing new targets for therapeutic interventions. Areas covered: This narrative review discusses the pathophysiologic link between CMD, GM, and potential microbiota-based targets against atherosclerosis and modifiable risk factors for atherosclerosis. Low-grade inflammation can be induced through GM and its derived metabolites. CMD are influenced by GM and microbiota-derived metabolites such as short-chain fatty acids (SCFA), secondary bile acids, trimethylamine N-oxide (TMAO), and the composition of GM can modulate host metabolism. All of the above can lead to promising therapeutic targets. Expert opinion: Most data are derived from animal models or human association studies; therefore, more translational and interventional research in humans is necessary to validate these promising findings. Reproduced findings such as aberrant microbiota patterns or circulating biomarkers could be targeted depending on individual metabolic profiles, moving toward personalized medicine in CMD. ARTICLE HISTORY

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Metabolome analysis for investigating host-gut microbiota interactions

Cited by 133 publications

References 111 publications

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

Exploring the Molecular Pathways Behind the Effects of Nutrients and Dietary Polyphenols on Gut Microbiota and Intestinal Permeability: A Perspective on the Potential of Metabolomics and Future Clinical Applications

Metabolomic profiling identifies complex lipid species associated with response to weight loss interventions

Gut microbiota: a promising target against cardiometabolic diseases

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