Metabolites of Trichoderma longibrachiatum EF5 inhibits soil borne pathogen, Macrophomina phaseolina by triggering amino sugar metabolism

Sridharan, A. P.; Thangappan, Sugitha; Karthikeyan, G.; Nakkeeran, S.; Uthandi, Sivakumar

doi:10.1016/j.micpath.2020.104714

Cited by 29 publications

(20 citation statements)

References 35 publications

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“…T. longibrachiatum is a soil-borne fungus belonging to the anamorphic genus Trichoderma (Hypocreales, Ascomycota). Several strains of T. longibrachiatum have been reported as promising biocontrol agents against phytopathogenic fungi, bacteria, and nematodes [15,[24][25][26][27][28]. To date, a variety of new antibiotics, including peptides, polyketides, and terpenes, have been isolated from T. longibrachiatum, which led to the recognition of the importance of T. longibrachiatum as a potential source of novel antibiotics [7,8,15].…”

Section: Structure Determination Of the Active Compounds Isolated From Trichoderma Longibrachiatum Sfc100166mentioning

confidence: 99%

In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

Ngo

Minh

Han

et al. 2021

JoF

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In the search for antifungal agents from marine resources, we recently found that the culture filtrate of Trichoderma longibrachiatum SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (1), and a new natural compound, 2′,3′-dihydro-epoxysorbicillinol (2), together with 11 known compounds (3–13), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, Phytophthora infestans was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine (9) and trichodimerol (10). When tomato plants were treated with the representative compounds (4, 6, 7, and 11), bisvertinolone (6) strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of T. longibrachiatum SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by P. infestans.

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Section: Structure Determination Of the Active Compounds Isolated From Trichoderma Longibrachiatum Sfc100166mentioning

confidence: 99%

In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

Ngo

Minh

Han

et al. 2021

JoF

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“…These secondary metabolites have antimicrobial features, acting as potential biocontrol agents against phytopathogenic antifungal activities (Vizcaino et al, 2005). Trichoderma longibrachiatum is one of the recent studied fungal biocontrol agents, to date, several strains have been reported to generate a diversity of new volatile and nonvolatile metabolites, including peptides, polyketides, and terpenes, which led to the appreciation of T. longibrachiatum as a potential source of unique antibiotics and as talented biocontrol agents against phytopathogenic microorganisms and nematodes (Sridharan et al, 2020(Sridharan et al, , 2021. These metabolites were found to delay the colonization of the pathogens; for instance, the antifungal secondary metabolites of T. longibrachiatum were reported against Candida albicans and Pyricularia oryzae (Xuan et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…During the direct T. longibrachiatum-pathogen interactions, an alteration and reduction of the mycelial growth and inhibited the production of sclerotia of the pathogens were noticed, which was associated with secretion of several antifungal compounds including longifolene, 1-butanol 2-methyl, cedrene, caryophyllene, and cuprenene, which are involved in the biosynthetic pathways of the sesquiterpenoid and alkane, and the degradation pathway of trimethylamine. What is more, 1-pentanol, 1-hexanol, myristonyl pantothenate, bisabolol, d-Alanine, and diethyl trisulphide draws attention as a plant-growth promoting and unique antimicrobial compounds (Sridharan et al, 2020(Sridharan et al, , 2021.…”

Section: Discussionmentioning

confidence: 99%

“…Superior to other fungi, endophytic Trichoderma spp. produce a novel array of metabolites that exhibit different bioactivities with diverse applications in pharmaceutical, industrial, plant growth regulation, and many others (De Silva et al, 2019;Sridharan et al, 2020Sridharan et al, , 2021. Therefore, this study is a trial to shed some light and share some information about such a limited-explored area.…”

Section: Introductionmentioning

confidence: 94%

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A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

et al. 2022

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Plant residuals comprise the natural habitat of the plant pathogen; therefore, attention is currently focusing on biological-based bioprocessing of biomass residuals into benefit substances. The current study focused on the biodegradation of peanut plant residual (PNR) into citric acid (CA) through a mathematical modeling strategy. Novel endophytic Trichoderma longibrachiatum WKA55 (GenBank accession number: MZ014020.1), having lytic (cellulase, protease, and polygalacturonase) activity, and tricalcium phosphate (TCP) solubilization ability were isolated from peanut seeds and used during the fermentation process. As reported by HPLC, the maximum CA (5505.1 μg/g PNR) was obtained after 9 days in the presence of 15.49 mg TCP, and 15.68 mg glucose. GC–MS analysis showed other bioactive metabolites in the filtrate of the fermented PNR. Practically, the crude product (40%) fully inhibited (100%) the growth and spore germination of three mycotoxinogenic fungi. On peanuts, it improved the seed germination (91%), seedling features, and vigor index (70.45%) with a reduction of abnormal seedlings (9.33%). The current study presents the fundamentals for large-scale production in the industry for the sustainable development of PNR biomass as a natural source of bioactive metabolites, and safe consumption of lignocellulosic-proteinaceous biomass, as well. T. longibrachiatum WKA55 was also introduced as a novel CA producer specified on PNR. Application of the resulting metabolite is encouraged on a large scale.

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“…and Phytophthora spp. [ 19 ]. Adding Trichoderma to fumigated soil can prolong the fumigant’s control of soil-borne pathogens when populations increase over time, compared with fumigation alone which becomes ineffective against pathogens as concentrations decline over time [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Beneficial effect on the soil microenvironment of Trichoderma applied after fumigation for cucumber production

Zhu

Zhang

et al. 2022

PLoS ONE

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Biocontrol agents applied after fumigation play an important role to the soil microenvironment. We studied the effect of Trichoderma applied after dimethyl disulfide (DMDS) plus chloropicrin (PIC) fumigation on the cucumber growth, soil physicochemical properties, enzyme activity, taxonomic diversity, and yield through laboratory and field experiments. The results confirmed that Trichoderma applied after fumigation significantly improved soil physicochemical properties, cucumber growth, soil-borne pathogens, and soil enzyme activity. Genetic analysis indicated that Trichoderma applied after fumigation significantly increased the relative abundance of Pseudomonas, Humicola and Chaetomium, and significantly decreased the relative abundance of the pathogens Fusarium spp. and Gibberella spp., which may help to control pathogens and enhanced the ecological functions of the soil. Moreover, Trichoderma applied after fumigation obviously improved cucumber yield (up to 35.6%), and increased relative efficacy of soil-borne pathogens (up to 99%) and root-knot nematodes (up to 96%). Especially, we found that Trichoderma applied after fumigation increased the relative abundance of some beneficial microorganisms (such as Sodiomyces and Rhizophlyctis) that can optimize soil microbiome. It is worth noting that with the decline in the impact of the fumigant, these beneficial microorganisms still maintain a higher abundance when the cucumber plants were uprooted. Importantly, we found one tested biocontrol agent Trichoderma 267 identified and stored in our laboratory not only improved cucumber growth, reduced soil-borne diseases in late cucumber growth stages but also optimized micro-ecological environment which may have good application prospect and help to keep environmental healthy and sustainable development.

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Metabolites of Trichoderma longibrachiatum EF5 inhibits soil borne pathogen, Macrophomina phaseolina by triggering amino sugar metabolism

Cited by 29 publications

References 35 publications

In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

Beneficial effect on the soil microenvironment of Trichoderma applied after fumigation for cucumber production

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