Metabolites of the pathogenic fungus Verticillium dahliae VI. Pentaketide metabolites and neutral lipids of virulent and avirulent strains

Ten, Leonid N.; Tyshchenko, A. A.; Stepanichenko, N. N.; Гусакова, С. Д.; Mukhamedzhanov, S. Z.; Otroshchenko, O. S.; Kas'yanenko, A. G.

doi:10.1007/bf00569579

Cited by 2 publications

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“…Aspergillus niger and six basidiomycetes do not synthesize DHN and apparently make a different type of melanin. Many studies have examined the pentaketide pathway of melanin synthesis in V. dahliae ; these studies have shown that this pathway is substrate specific and occurs during a certain developmental morphology (melanized microsclerotia) associated with the life cycle of the fungus (Bell et al ., ; Stipanovic and Bell, ; Ten et al ., ; Tokousbalides and Sisler, ; Wheeler et al ., ). Microsclerotia are the resting structures of V. dahliae and can endure desiccation and cold, thus facilitating the long‐term survival of V. dahliae without its host plant.…”

Section: Discussionmentioning

confidence: 99%

The fungal‐specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae

Luo

Mao

Wei

et al. 2016

Molecular Plant Pathology

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Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transcription factor-encoding gene Vdpf was shown to be associated with vegetative growth and virulence, with the highest transcript expression occurring during conidia formation in the V991 strain. The deletion mutants (ΔVdpf) and insertion mutants (IMΔVdpf) produced fewer conidia than did the wild-type (WT) fungi, which contributed to the reduced virulence. Unlike the WT, the complemented strains and IMΔVdpf, ΔVdpf formed swollen, thick-walled and hyaline mycelium rather than melanized microsclerotia. The ΔVdpf mutants were melanin deficient, with undetectable expression of melanin biosynthesis-related genes (Brn1, Brn2 and Scd1). The melanin deficiency was related to cyclic adenosine monophosphate (cAMP) and the G-protein-coupled signalling pathways in this study. Similar to the WT and complemented strains, the ΔVdpf and IMΔVdpf mutants could also successfully penetrate into cotton and tobacco roots, but displayed reduced virulence because of lower biomass in the plant roots and significantly reduced expression of pathogenicity-related genes in V. dahliae. In conclusion, these results provide insights into the role of Vdpf in melanized microsclerotia formation, conidia production and pathogenicity.

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