Metabolites of Oxytetracycline, Tetracycline, and Chlortetracycline and Their Distribution in Egg White, Egg Yolk, and Hen Plasma

Zurhelle, Georg; Petz, Michael; Mueller-Seitz, Erika; Siewert, Eike

doi:10.1021/jf000141k

Cited by 36 publications

(20 citation statements)

References 10 publications

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“…They found that the drugs were present in greater amounts in the egg yolk than in the white and also remained for longer in the yolk. Vandenberge et al (2012) and Zurhelle, Petz, Mueller-Seitz, and Siewert (2000) also reported similar results. Further, Alaboudi, Bash, and Musallam (2013) reported finding higher concentrations of chlortetracycline in egg white than in yolk.…”

Section: Toxicology and Chemical Food Safetysupporting

confidence: 64%

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Evaluation of Tilmicosin Contamination in Eggs Following Its Administration to Laying Hens and Subsequent Assessment of Dietary Risks to Chinese Consumers

Yang

Wang

et al. 2019

Journal of Food Science

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In the present study, we analyzed the tilmicosin residues in eggs as well as the dietary risks posed by the residual drug to Chinese consumers. Tilmicosin was administrated to laying hens via drinking water in doses of 100 mg/L and 300 mg/L (dose 1 and dose 2) for 5 days. Its residues distribution within egg matrices were detected using a QuEChERS method coupled with liquid chromatography–tandem mass spectrometry (LC‐MS/MS). The results indicated that the tilmicosin residue was the highest in the whole eggs and yolks on day 2 after medication and in the whites on day 5 of drug administration for dose 1 and dose 2 with the residues (µg/kg) being the following: 73.4 and 444.3 in the whole eggs; 152.0 and 1141.1 in the yolks; and 48.8 and 277.6 in the whites, respectively. During withdrawal phase, the tilmicosin levels declined to less than the limit of detection (LOD) after 29 days and 49 days for the whole eggs, after 24 days and 44 days for the yolks, and after 19 days and 49 days for the whites for doses 1 and 2, respectively. An assessment of the dietary risk for Chinese consumers revealed that the hazard quotients (HQ) values for tilmicosin consumption were less than 1 for dose 1 and greater than 1 for dose 2, with children (2 to 7 years) and adult males (over 65 years) being the high‐risk groups. These results suggest that the possible risk associated with tilmicosin contamination in eggs should not be ignored since the accidental or occasional misuse of tilmicosin in the case of egg‐laying hens may occur from time to time, particularly on small farms. Practical Application The modified QuEChERS method was used to analyze the tilmicosin residues in egg matrices and a subsequent dietary exposure assessment for Chinese consumers was performed, which can serve as a reference for the food safety risk posed by antibiotic misuse in egg layers.

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Section: Toxicology and Chemical Food Safetysupporting

confidence: 64%

“…Vandenberge et al. () and Zurhelle, Petz, Mueller‐Seitz, and Siewert () also reported similar results. Further, Alaboudi, Bash, and Musallam () reported finding higher concentrations of chlortetracycline in egg white than in yolk.…”

Section: Resultsmentioning

confidence: 59%

Evaluation of Tilmicosin Contamination in Eggs Following Its Administration to Laying Hens and Subsequent Assessment of Dietary Risks to Chinese Consumers

Yang

Wang

et al. 2019

Journal of Food Science

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“…When administrated to layer chickens, drug residues appear more firstly in albumen than in yolk egg but concentrations could reach higher levels and persist in yolk [36][37][38][39]. During the present study, albumen and yolk egg were not separated during screening process and this could be the reason of the weak level of tetracycline residues in eggs tested.…”

Section: Screening Of β-Lactam and Tetracycline Residues In Tissue Anmentioning

confidence: 72%

Practices of Usage of Antibiotics in Chicken Farming and Impact of Some of their Residues in Products Consumed in Yaoundé, Cameroon

2019

ANFS

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Background: Veterinary antibiotics are drugs widely used against bacterial infections in animals such as chickens, for infections prevention or treatment. But it was noticed that foodstuffs contaminated with antimicrobial residues may cause diverse side effects to consumers, when their levels are exceeding the standards and this occurs when antibiotics are poorly used. To date, data regarding antimicrobial usage in chicken farming and their levels in chicken products are not almost available. A survey was conducted to a hundred of farmers in twenty seven localities of Yaoundé, the political capital of Cameroon to describe the usage practices of antibiotics. Tissue and egg samples were randomly collected in five markets of the same town to appraise their compliance for tetracyclines and β-lactams with standards (not exceeding maximum residues limits) using the radio-receptor technique (Charm II Test). Results: The results showed that 92.1 % of farms were using antibiotics via drinking water administration. Among the antibiotics used, tetracycline’s represent 66.7 %, quinolones 44.4 %, β-lactams 22.2 % and nitro furans 11.1 %. Diseases were recorded in the majority of the farms during the survey and they include cough (92.1 %), gomboro (27.7 %), parasitosis (25.7 %) and pullorose (25.7 %).The withdrawal period was not following by 35.6 % of the farmers, 43.5 % of farms were not respecting the administration dose while 50.5 % were not having an appropriate place for the storage of antibiotics. 61.9 % of liver, 38.1 % of gizzard, 33.3 % of muscle and 8.8 % of egg samples contained tetracycline residues above the maximum residue limits (MRLs) (200 µg /Kg for tetracycline residue and 50 µg /Kg for β-lactam). Contrarily, none of the samples were none compliance for β-lactam residues. Conclusion: These results suggest that, poorly use of antibiotics in rearing of chickens in Yaoundé (Cameroon) presents a risk for consumers. It is therefore important to inform the farmers about recommended standards for the use of these antibiotics in order to guarantee consumer food safety

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“…Previous results showed that oxytetracycline breaks down to several metabolites, including 4-epi-oxytetracycline. For example, 4-epi-oxytetracycline and N-demethyloxytetracycline were detected as metabolites of oxytetracycline in egg and hen plasma [11]. In another study, 4-epioxytetracycline was also detected in bones (femur, breastbone, fibula, and tibia) from broilers treated with oxytetracycline [12].…”

Section: Discussionmentioning

confidence: 96%

Evaluation of Oxytetracycline Metabolites Cross-Reactivity with Oxytetracycline Enzyme-Linked Immunosorbent Assay (ELISA)

Hijaz

Killiny

2020

Antibiotics

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Antibiotics have been successfully used for the control of several plant diseases for many years. Recently, streptomycin and oxytetracycline have been approved for the treatment of Huanglongbing (HLB) in Florida. The enzyme-linked immunosorbent assay (ELISA) is the most commonly used assay for the detection of these antibiotics because it is quick, simple, and can be used to analyze many samples at the same time. However, ELISA can react with the metabolites of the parent compound and its structurally related compounds. In this study, we investigated the cross-reactivity of the oxytetracycline ACCEL ELISA kit TM with three of oxytetracycline metabolites (4-epi-oxytetracycline, α-apo-oxytetracycline, and β-apo-oxytetracycline). The α-apo-oxytetracycline and β-apo-oxytetracycline metabolite did not show any cross-reactivity in the linear range (1.5-50 ng mL −1 ) of the assay. Whereas 4-epi-oxytetracycline showed high cross-reactivity, and its response was similar to oxytetracycline. Our results indicated that the oxytetracycline ELISA kits estimate the level of oxytetracycline as well as its main metabolite, 4-epi-oxytetracycline.

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Metabolites of Oxytetracycline, Tetracycline, and Chlortetracycline and Their Distribution in Egg White, Egg Yolk, and Hen Plasma

Cited by 36 publications

References 10 publications

Evaluation of Tilmicosin Contamination in Eggs Following Its Administration to Laying Hens and Subsequent Assessment of Dietary Risks to Chinese Consumers

Evaluation of Tilmicosin Contamination in Eggs Following Its Administration to Laying Hens and Subsequent Assessment of Dietary Risks to Chinese Consumers

Practices of Usage of Antibiotics in Chicken Farming and Impact of Some of their Residues in Products Consumed in Yaoundé, Cameroon

Evaluation of Oxytetracycline Metabolites Cross-Reactivity with Oxytetracycline Enzyme-Linked Immunosorbent Assay (ELISA)

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