Metabolites in cultured cells of Ibervillea sonorae (S. Watson) Greene display increased hypoglycemic activity compared to that seen in plant roots

Anti-hyperglycemic activity of aqueous extracts of cells produced in Ibervillea sonorae(S. Watson) Green cell culture on murine model

“…We also obtained a total flavonoid content of 1.26 ± 0.01 mg Rutin/g of extract at 21 days of culture. These values of flavonoids are similar to those obtained by Arciniega-Carreón et al (2020), from I. sonorae suspended cell cultures in a flask (from stem explant), in which they obtained 1.10 ± 0.03 mg Rutin/g. In other studies, the concentration of flavonoids was higher than that obtained with other Cucurbitaceae in callus extracts from Momordia charantia L. where 2.90 mg/g of flavonoids biomass (kaempferol, quercetin, and luteolin) were produced at 42 days of culture (Ahmed et al, 2015), such as -tocopherol (42.93 µg/g FW), and total folate (0.72 µg/g FW) (Saini & Keum, 2017).…”

“…Once the stems and leaves grow, some stem explants were disinfected according to the procedure modified by Estrada-Zúñiga et al (2012). were cultured in a stirred tank bioreactor of 0.5 L with a B5 medium (Gamborg et al, 1968), according to the methodology described by Arciniega-Carreón et al (2020), 28 days, cells cultures from stem explants of I. sonorae were called CCSE-Is. At the end of kinetics, CCSE-Is were lyophilized (Labconco-051145636-A) at 0.0360 mbar vacuum, upper temperature: -10°C, lower temperature: -80°C and 0.0360 mbar, for 24 h for further studies.…”

“…The production of fatty acids of callus of leaf explants in vitro cultures of I. sonorae was obtained, and its anti-oxidant activity was evaluated (Estrada-Zúñiga et al, 2012). Our group has tested that in the extracts of I. sonorae cell cultures, the flask stimulated glucose uptake is more efficacious than plant roots extract (Arciniega-Carreón et al, 2020).…”

“…Various studies regarding the I. sonorae root extract have identified the presence of metabolites with pharmacological activity such as alkaloids, coumarins, flavonoids, saponins, quinones, fatty acids (Morales & Siles, 2013;Zapata-Bustos et al, 2014), cucurbitacines (Achenbach et al, 1993;Torres-Moreno et al, 2015;Tripathi et al, 2019;Torres-Moreno et al, 2020), and kinoin A, B (Jardón-Delgado et al, 2014). The production of phenols and flavonoids has been quantified in flask cell cultures of explants stem cultured (Arciniega-Carreón et al, 2020). Therefore, this study aimed to establish cell cultures from explant stems of I. sonorae in a 0.5 L stirred tank bioreactor to obtain fatty acids, phenols, and flavonoids, as an alternative to establishing a consistent source of these kinds of metabolites; as well as the evaluation of the antihyperglycemic activity of the total cell extracts cultured from the bioreactor in a murine model with streptozotocin-induced Diabetes.…”