Metabolites from the Fungal Endophyte <i>Aspergillus austroafricanus</i> in Axenic Culture and in Fungal–Bacterial Mixed Cultures

Ebrahim, Weaam; El-Neketi, Mona; Lewald, Laura-Isabell; Orfali, Raha; Lin, Wenhan; Rehberg, Nidja; Kalscheuer, Rainer; Δαλέτος, Γεώργιος; Proksch, Peter

doi:10.1021/acs.jnatprod.5b00975

Cited by 84 publications

(46 citation statements)

References 39 publications

(74 reference statements)

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“…Gliomastin A (1) features a new carbon skeleton, which could be derived from two co-isolated known hydroquinone derivatives acremonin A (11) 24 and F-11334A 1 (13). 25,26 A biosynthetic pathway from the latter two compounds to 1 via Diels-Alder reaction is proposed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.

et al. 2017

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Eight new hydroquinone derivatives, gliomastins A-D (1-4), 9-O-methylgliomastin C (5), acremonin A 1-Ob-D-glucopyranoside (6), gliomastin E 1-O-b-D-glucopyranoside (7), and 6together with seven known analogues were isolated from the marine-derived fungus Gliomastix sp. Their structures were elucidated by extensive spectroscopic analysis including 1D and 2D NMR measurements aided by DFT NMR calculations as well as MS data. TDDFT-ECD and OR calculations were performed to determine the absolute configurations of 1 and the aglycones of 6 and 7. Compound 1 features a novel skeleton, biogenetically derived from a Diels-Alder reaction between derivatives of 11 and 13. Compound 2 represents a rare sulfur-containing alkaloid derived from the known hydroquinone 13.Compounds 1, 10 and 12 showed strong cytotoxicity against the L5178Y mouse lymphoma cell line with IC 50 values of 1.8, 1.0 and 1.1 mM, respectively. Compound 3 exhibited moderate antitubercular activity against Mycobacterium tuberculosis with a MIC value of 12.5 mM.

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Section: Resultsmentioning

confidence: 99%

Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.

et al. 2017

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“…There are a number of recent studies which have demonstrated that co-cultivation is a remarkably successful approach for the discovery of new bioactive natural products (Onaka et al, 2011; Rateb et al, 2013; Dashti et al, 2014; Ebrahim et al, 2016), and an overview is provided by Marmann et al (2014). Moreover, microbial co-cultivation has been shown to increase the titre of a specific metabolite in some cases, for example for the yew tree fungal endophyte Paraconiothyrium , responsible for production of the potent anticancer drug paclitaxel.…”

Section: Introductionmentioning

confidence: 99%

Dual Induction of New Microbial Secondary Metabolites by Fungal Bacterial Co-cultivation

et al. 2017

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The frequent re-isolation of known compounds is one of the major challenges in drug discovery. Many biosynthetic genes are not expressed under standard culture conditions, thus limiting the chemical diversity of microbial compounds that can be obtained through fermentation. On the other hand, the competition during co-cultivation of two or more different microorganisms in most cases leads to an enhanced production of constitutively present compounds or an accumulation of cryptic compounds that are not detected in axenic cultures of the producing strain under different fermentation conditions. Herein, we report the dual induction of newly detected bacterial and fungal metabolites by the co-cultivation of the marine-derived fungal isolate Aspergillus fumigatus MR2012 and two hyper-arid desert bacterial isolates Streptomyces leeuwenhoekii strain C34 and strain C58. Co-cultivation of the fungal isolate MR2012 with the bacterial strain C34 led to the production of luteoride D, a new luteoride derivative and pseurotin G, a new pseurotin derivative in addition to the production of terezine D and 11-O-methylpseurotin A which were not traced before from this fungal strain under different fermentation conditions. In addition to the previously detected metabolites in strain C34, the lasso peptide chaxapeptin was isolated under co-culture conditions. The gene cluster for the latter compound had been identified through genome scanning, but it had never been detected before in the axenic culture of strain C34. Furthermore, when the fungus MR2012 was co-cultivated with the bacterial strain C58, the main producer of chaxapeptin, the titre of this metabolite was doubled, while additionally the bacterial metabolite pentalenic acid was detected and isolated for the first time from this strain, whereas the major fungal metabolites that were produced under axenic culture were suppressed. Finally, fermentation of the MR2012 by itself led to the isolation of the new diketopiperazine metabolite named brevianamide X.

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“…Fungi are reported to be potential color producer [2]. Various natural dyes from fungi were reported.…”

Section: Introductionmentioning

confidence: 99%

Coloring Properties Assessment of Dyes Produced by MixedAspergillusandPaecilomyces

Suciatmih

Nurfianti

Magfirani

2018

IOP Conf. Ser.: Earth Environ. Sci.

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Metabolites from the Fungal Endophyte Aspergillus austroafricanus in Axenic Culture and in Fungal–Bacterial Mixed Cultures

Cited by 84 publications

References 39 publications

Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.

Hydroquinone derivatives from the marine-derived fungus Gliomastix sp.

Dual Induction of New Microbial Secondary Metabolites by Fungal Bacterial Co-cultivation

Coloring Properties Assessment of Dyes Produced by MixedAspergillusandPaecilomyces

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