Metabolite Variation in Lean and Obese Streptozotocin (STZ)-Induced Diabetic Rats via 1H NMR-Based Metabolomics Approach

Sajak, Azliana Abu Bakar; Mediani, Ahmed; Maulidiani, M.; Ismail, Amin; Abas, Faridah

doi:10.1007/s12010-016-2352-9

Cited by 23 publications

(12 citation statements)

References 39 publications

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“…In animal studies, high levels of glucose are shown in the metabolic profile. For example, Abu Bakar Sajak et al [26] and Mulidiani et al [25] detected and quantified glucose in urine in streptozotocin (STZ)-induced diabetic rats. In recent clinical studies, glucose is significantly increased in adults with risk to develop MetS or related diseases [95].…”

Section: Glucosementioning

confidence: 99%

“…BCAAs and AAAs have been related to MetS, obesity, and T2D in animal models and in human studies, both longitudinal and cross-sectional studies, having in common the usage of NMR metabolic profiles. For example, elevated levels of BCAAs and AAAs have been reported between diabetes and control group in STZ rat model [25,26]. In a longitudinal human studies, BCAAs were associated with higher glycaemia and IR and post-challenge glucose levels using NMR approach [32].…”

Section: Bcaasmentioning

confidence: 99%

“…In STD-rats, allantoin levels in urine stand out, among other metabolites, in T2D and obesity risk factor [196]. In a project characterizing biomarkers associated with T2D in eighteen biological matrices in db/db mouse model, allantoin was elevated in urine and plasma [26]. In other study characterizing the urine metabolome between lean and overweight dogs during a feed-challenge, overweight dogs had higher postprandial allantoin concentrations compared with lean dogs [76].…”

Section: Uric Acid and Allantoinmentioning

confidence: 99%

“…Then we will discuss the involvement of new biomarkers in the early stages of MetS distributed in the following list of molecular clusters: carbohydrate metabolism, dyslipidemia, inflammation, oxidative stress, and gut microbiota dysbiosis. [25,26] [ 19,27,28] Lactate Increased Serum, urine Carbohydrate disruption Gluconeogenesis, Pyruvate metabolism [29,30] [27, 31,32] Increased Urine Gut microbiota metabolism [33] Uric [81,82] Branched chain aminoacids (BCAAs); aromatic aminoacids (AAAs); trimethylamine N-oxide (TMAO); trimethylamine (TMA); dimethylamine (DMA); lysophospholipids (LPCs); N-acetylglycoproteins (NAG); saturated fatty acids (SFA); monounsaturated fatty acids (MUFAs); polyunsaturated fatty acids (PUFAs); eicosapentaenoic acid (EPA); docosahexaenoic acid (DHA); arachidonic acid (ARA); arachidonic acid (AA).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

et al. 2020

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The metabolic syndrome is a multifactorial disease developed due to accumulation and chronification of several risk factors associated with disrupted metabolism. The early detection of the biomarkers by NMR spectroscopy could be helpful to prevent multifactorial diseases. The exposure of each risk factor can be detected by traditional molecular markers but the current biomarkers have not been enough precise to detect the primary stages of disease. Thus, there is a need to obtain novel molecular markers of pre-disease stages. A promising source of new molecular markers are metabolomics standing out the research of biomarkers in NMR approaches. An increasing number of nutritionists integrate metabolomics into their study design, making nutrimetabolomics one of the most promising avenues for improving personalized nutrition. This review highlight the major five risk factors associated with metabolic syndrome and related diseases including carbohydrate dysfunction, dyslipidemia, oxidative stress, inflammation, and gut microbiota dysbiosis. Together, it is proposed a profile of metabolites of each risk factor obtained from NMR approaches to target them using personalized nutrition, which will improve the quality of life for these patients.

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Section: Glucosementioning

confidence: 99%

Section: Bcaasmentioning

confidence: 99%

Section: Uric Acid and Allantoinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

et al. 2020

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“…Furthermore, inhibition of IRS-1 can suppress the activation of protein kinase B (AKT), thus, affecting the glucose transport and protein synthesis and causing IR in skeletal muscle via negative feedback. One study found that the level of BCAA increased significantly in the plasma of T2DM rats (Sajak, Mediani, Ismail, & Abas, 2017).…”

Section: Discussionmentioning

confidence: 99%

Zanthoxylum alkylamides improve amino acid metabolism in type 2 diabetes mellitus rats

et al. 2020

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To investigate the effect of Zanthoxylum alkylamides (ZA) on amino acid metabolism of type 2 diabetes mellitus (T2DM), the present study was performed with T2DM rats model induced with high fat and sugar fodder combined with low-dose of streptozotocin. ZA were fed to rats at three different doses of 2, 4, and 8 mg/kg for 28 days and metformin was fed to rats at 135 mg/kg as positive control. The results showed that compared with the normal control, the amino acid levels and the expression of related carrier genes were disordered in T2DM rats. Compared with the model, different doses of ZA could significantly resist (p < .05) the decrease in body weight of T2DM rats and improve hyperglycemia, with the best result observed with the high dose (8 mg/kg). Different doses of ZA could ameliorate the levels of 19 kinds of amino acid in the plasma, jejunum, liver, and skeletal muscle of T2DM rats by regulating the expression of related amino acid transporters including LAT1, SNAT2, CAT1, et al. to thereby ameliorating amino acid metabolism disorder in T2DM rats. Practical applications Previous studies showed that Zanthoxylum alkylamides (ZA) could promote the amino acid metabolism in the jejunum of healthy SD rats, improve protein metabolism disorder of type 1 diabetic rats, and also reduce the risk of metabolic syndrome in fat rats model. Herein, we investigated the effect of ZA on amino acid metabolism in type 2 diabetes mellitus (T2DM) rats. The results indicated that ZA could remarkably improve the abnormal expression of amino acid carriers in the jejunum, liver, and skeletal muscle, thereby ameliorating the disorder of amino acid metabolism in the plasma, jejunum, liver, and skeletal muscle of T2DM rats. Therefore, ZA are potential antidiabetic food/medicine product for the T2DM treatment.

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Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats

Majid

Hamid

Salleh

et al. 2019

Phytochemical Analysis

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Introduction Natural products are obtaining much acceptance as ergogenic aid, not only among athletes but also among the general population including people with excess body fat. Under normal circumstances, an obese person will have the desire and ability to exercise reduced; mainly because they are easily fatigued. Thus, they need to boost their energy production so that they can be more active and healthier. Objective In this present work, Morinda citrifolia L. leaf extract (MLE) which is believed to possess ergogenic property, was evaluated on its effect on an obese animal model using 1H‐NMR based metabolomics. Material and Methods Rats were fed with high fat diet (HFD) for 12 weeks for obese development. Once this was achieved, all the rats underwent endurance exercise (forced swimming test) every 2 weeks for 8 weeks together with treatment. The time to exhaustion was recorded for each rat. Three different dosages of MLE: 50 mg/kg, 100 mg/kg and 200 mg/kg of body weight were used together with two positive controls: 5 mg/kg caffeine and 100 mg/kg green tea. Blood was collected before and after treatments for metabolomics study. Results Findings showed that feeding the rats at a dose of 200 mg/kg body weight MLE significantly prolonged the exhaustive swimming time of the rats, and altered the metabolites present in their serum. Discriminating metabolites involved were the product of various metabolic pathways, including carbohydrate, lipids metabolism and energy metabolism. Treatment with 200 mg/kg body weight MLE resulted in significant improvement in the metabolic perturbations where the proximity of the obese exercised treated group to that of normal exercised group in the partial least squares discriminant analysis score plot was observed. Conclusion The present work demonstrated ergogenic property of MLE based on the improved metabolic perturbation in exercised obese rats.

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Metabolite Variation in Lean and Obese Streptozotocin (STZ)-Induced Diabetic Rats via 1H NMR-Based Metabolomics Approach

Cited by 23 publications

References 39 publications

Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

Detection of Early Disease Risk Factors Associated with Metabolic Syndrome: A New Era with the NMR Metabolomics Assessment

Zanthoxylum alkylamides improve amino acid metabolism in type 2 diabetes mellitus rats

Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats

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