Metabolite Profiling Reveals the Effect of Dietary Rubus coreanus Vinegar on Ovariectomy-Induced Osteoporosis in a Rat Model

Lee, Mee Youn; Kim, Hyang Yeon; Singh, Devendra; Yeo, Soo Hwan; Baek, Seong Yeol; Park, Yoo Kyoung; Lee, Choong Hwan

doi:10.3390/molecules21020149

“…Likewise, the significant increased levels of lysoPCs were also found in Liu’s study [40]. These lysoPCs likely enhanced oxidative stress by generating reactive oxygen species (ROS), the production of which disturbed the balance of the antioxidant–oxidation process, and the associated oxidative stress may lead to extensive bone loss and skeletal fragility [39]. Further, the disturbance of fatty acid metabolism also causes bone loss.…”

Section: Biological and Metabolic Significance Of The Biomarkersmentioning

confidence: 65%

“…Lee et al have shown metabolic differences between OVX SD rats and sham rats based on GC/MS spectra of plasma samples through data analysis using principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA). Three potential biomarkers were mainly identified as lower concentrations of phenylalanine, tryptophan and butyric acid [39]. Ma et al compared metabolic perturbations, as measured by GC/MS, between OVX SD rats and sham rats.…”

Section: The Biomarkers and Their Potential Values In Osteoporosismentioning

confidence: 99%

“…Vinegar, which is a rich source of minerals, could enhance intestinal calcium absorption and reduce bone turnover in preventing osteoporosis [67]. Lee et al found that the Rubus coreanus vinegar could be served as a potential dietary supplement to counter the progression of osteoporosis as for the metabolomics results revealed that the metabolites in the plasma returned to a normal level in OVX rats after the administration of Rubus coreanus vinegar [39]. …”

Section: The Biomarkers and Their Potential Values In Osteoporosismentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics and Its Application in the Development of Discovering Biomarkers for Osteoporosis Research

Lv

¹

,

Jiang

²

,

Guan

³

et al. 2016

IJMS

View full text Add to dashboard Cite

Osteoporosis is a progressive skeletal disorder characterized by low bone mass and increased risk of fracture in later life. The incidence and costs associated with treating osteoporosis cause heavy socio-economic burden. Currently, the diagnosis of osteoporosis mainly depends on bone mineral density and bone turnover markers. However, these indexes are not sensitive and accurate enough to reflect the osteoporosis progression. Metabolomics offers the potential for a holistic approach for clinical diagnoses and treatment, as well as understanding of the pathological mechanism of osteoporosis. In this review, we firstly describe the study subjects of osteoporosis and bio-sample preparation procedures for different analytic purposes, followed by illustrating the biomarkers with potentially predictive, diagnosis and pharmaceutical values when applied in osteoporosis research. Then, we summarize the published metabolic pathways related to osteoporosis. Furthermore, we discuss the importance of chronological data and combination of multi-omics in fully understanding osteoporosis. The application of metabolomics in osteoporosis could provide researchers the opportunity to gain new insight into the metabolic profiling and pathophysiological mechanisms. However, there is still much to be done to validate the potential biomarkers responsible for the progression of osteoporosis and there are still many details needed to be further elucidated.

show abstract

“…Here, the rats were 13 weeks old at the time of ovariectomy, which is reportedly acceptable for the osteoporosis model [45, 46]. Furthermore, Francisco et al compared the effects of ovariectomy in rats of different ages and found no related differences in bone mineral response [47].…”

Section: Discussionmentioning

confidence: 99%

Effect of the Lipoxygenase Inhibitor Baicalein on Muscles in Ovariectomized Rats

Saul

¹

,

Kling

²

,

Kosinsky

³

et al. 2016

Journal of Nutrition and Metabolism

View full text Add to dashboard Cite

Sarcopenia, a loss of muscle mass accompanying osteoporosis, leads to falls and fall-related injuries. Baicalein, as a phytochemical agent, has an antioxidative and anti-inflammatory effect in muscle. In this study, sixty-one female Sprague Dawley rats were divided into five groups: four groups were ovariectomized (OVX) and one control group was nonovariectomized (NON-OVX). Eight weeks after ovariectomy, three disparate concentrations (1 mg/kg body weight (BW), 10 mg/kg BW, and 100 mg/kg BW) of baicalein were applied subcutaneously daily in three OVX groups. Mm. soleus, gastrocnemius, and longissimus were extracted; their diameter, area, relation to body, and muscle weights as well as number of capillaries per fibre were recorded. In Mm. soleus and gastrocnemius, the baicalein effect (increasing number of capillaries per fibre) was proportional to the dose applied. The fibre diameters and area under baicalein treatment were significantly greater compared to OVX and NON-OVX groups. In M. longissimus, we observed a shift to type IIa fibres. Serum creatine kinase levels were significantly lower in highest baicalein concentration group. We conclude that baicalein can stimulate angiogenesis, though not fibre type-specific, in skeletal muscle and reduce the estrogen-related loss of fibre diameter and area in the skeletal muscle in rats. Therefore, a protective effect of baicalein on muscle cells can be assumed.

show abstract

“…Sprague Dawley rats are a valuable model for the induction of osteoporosis after hormonal depletion via ovariectomy [43,44]. Here, the rats were 13 weeks old at the time of ovariectomy, which is reportedly acceptable for the osteoporosis model [45,46]. Furthermore, Francisco et al compared the effects of ovariectomy in rats of different ages and found no related differences in bone mineral response [47].…”

Section: Discussionmentioning

confidence: 99%

Effect of the lipoxygenase inhibitor baicalein on muscles in ovariectomized rats

Saul¹

2017

Preprint

View full text Add to dashboard Cite

Sarcopenia, a loss of muscle mass accompanying osteoporosis, leads to falls and fall-related injuries. Baicalein, as a phytochemical agent, has an antioxidative and anti-inflammatory effect in muscle. In this study, sixty-one female Sprague Dawley rats were divided into five groups: four groups were ovariectomized (OVX) and one control group was nonovariectomized (NON-OVX). Eight weeks after ovariectomy, three disparate concentrations (1 mg/kg body weight (BW), 10 mg/kg BW, and 100 mg/kg BW) of baicalein were applied subcutaneously daily in three OVX groups. Mm. soleus, gastrocnemius, and longissimus were extracted; their diameter, area, relation to body, and muscle weights as well as number of capillaries per fibre were recorded. In Mm. soleus and gastrocnemius, the baicalein effect (increasing number of capillaries per fibre) was proportional to the dose applied. The fibre diameters and area under baicalein treatment were significantly greater compared to OVX and NON-OVX groups. In M. longissimus, we observed a shift to type IIa fibres. Serum creatine kinase levels were significantly lower in highest baicalein concentration group. We conclude that baicalein can stimulate angiogenesis, though not fibre type-specific, in skeletal muscle and reduce the estrogen-related loss of fibre diameter and area in the skeletal muscle in rats. Therefore, a protective effect of baicalein on muscle cells can be assumed.

show abstract

Metabolite Profiling Reveals the Effect of Dietary Rubus coreanus Vinegar on Ovariectomy-Induced Osteoporosis in a Rat Model

Cited by 31 publications

References 58 publications

Metabolomics and Its Application in the Development of Discovering Biomarkers for Osteoporosis Research

Metabolomics and Its Application in the Development of Discovering Biomarkers for Osteoporosis Research

Effect of the Lipoxygenase Inhibitor Baicalein on Muscles in Ovariectomized Rats

Effect of the lipoxygenase inhibitor baicalein on muscles in ovariectomized rats

Contact Info

Product

Resources

About