Metabolite profiling of sex developmental steroid conjugates reveals an association between decreased levels of steroid sulfates and adiposity in obese girls

Lee, Su Hyeon; Kim, Shin Hye; Lee, Won Yong; Chung, Bong Chul; Park, Mi Jung; Choi, Man Ho

doi:10.1016/j.jsbmb.2016.04.020

Cited by 8 publications

(14 citation statements)

References 27 publications

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“…Two studies specifically targeted steroid hormones. Lee et al 40 reported lower levels of DHEA‐S, pregnenolone sulfate, cholesterol sulfate among Korean girls with obesity, suggesting alteration to steroid phase II metabolism. Mauras et al 41 applied a targeted LC‐MS assay of 12 Estrogens and their metabolites in 35 prepubertal American girls.…”

Section: Resultsmentioning

confidence: 99%

“…Increases in steroid hormones, in particular androgens, were reported by multiple studies, including 4‐Androsten‐3b‐17b‐diol disulfate, 19 , 18 5a‐Androstan‐3b‐17b‐diol disulfate, 19 , 20 androsteroid monosulfate, 19 , 18 and DHEA‐S, 19 , 18 , 20 although one study also reported a decrease in DHEA‐S 40 . DHEA‐S was also reported to be decreased in a study of girls following weight‐loss 56 .…”

Section: Discussionmentioning

confidence: 98%

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A systematic review of metabolomic studies of childhood obesity: State of the evidence for metabolic determinants and consequences

et al. 2021

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Summary Childhood obesity has become a global epidemic and carries significant long‐term consequences to physical and mental health. Metabolomics, the global profiling of small molecules or metabolites, may reveal the mechanisms of development of childhood obesity and clarify links between obesity and metabolic disease. A systematic review of metabolomic studies of childhood obesity was conducted, following Preferred Reporting Items for Systematic Reviews (PRISMA) guidelines, searching across Scopus, Ovid, Web of Science and PubMed databases for articles published from January 1, 2005 to July 8, 2020, retrieving 1271 different records and retaining 41 articles for qualitative synthesis. Study quality was assessed using a modified Newcastle–Ottawa Scale. Thirty‐three studies were conducted on blood, six on urine, three on umbilical cord blood, and one on saliva. Thirty studies were primarily cross‐sectional, five studies were primarily longitudinal, and seven studies examined effects of weight‐loss following a life‐style intervention. A consistent metabolic profile of childhood obesity was observed including amino acids (particularly branched chain and aromatic), carnitines, lipids, and steroids. Although the use of metabolomics in childhood obesity research is still developing, the identified metabolites have provided additional insight into the pathogenesis of many obesity‐related diseases. Further longitudinal research is needed into the role of metabolic profiles and child obesity risk.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

A systematic review of metabolomic studies of childhood obesity: State of the evidence for metabolic determinants and consequences

et al. 2021

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“…These results obtained based on the ICH guidelines showed that the IT-SPME LC−MS/MS method has good linearity and precision. The method is simpler, more sensitive, and more specific than previously reported methods [ 6 , 7 , 16 , 17 , 18 , 19 , 20 , 21 , 25 ] and can be fully automated from extraction and concentration of the four sulfated steroid metabolites to their separation and analysis.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, some of these sulfated steroids are desulfated by steroid sulfatases (STSs), with these steroids returning to their free forms [ 1 , 3 ]. This cycle plays an important role in the regulation of total steroid content and bioactivity in vivo [ 4 , 5 ], and the lack of STSs is a major factor in the pathogenesis of STS deficiency, recessive X-linked ichthyosis (RXLI), and the metabolic syndrome [ 6 , 7 ]. The ability to accurately measure the concentrations of sulfated steroid metabolites in vivo can not only provide insight into the regulation of steroid metabolism by the balance between SULTs and STSs, but also lead to the diagnosis of various diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Chemical cleavage of sulfated steroid metabolites also results in the hydrolysis of other conjugates, and commercially available sulfatase enzymes, which enzymatically cleave these sulfated compounds, also possess glucuronidase activity, making it difficult to distinguish between steroid glucuronides and sulfated steroids [ 6 , 14 ]. In contrast, liquid chromatography–mass spectrometry (LC–MS) and LC–tandem mass spectrometry (MS/MS) exhibit excellent ionization properties via electrospray ionization and do not require derivatization of compounds, and therefore the specific fragmentation patterns of these compounds allow selective and sensitive analysis by MS/MS [ 1 , 2 , 3 , 4 , 6 , 7 , 10 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Online In-Tube Solid-Phase Microextraction Coupled with Liquid Chromatography–Tandem Mass Spectrometry for Automated Analysis of Four Sulfated Steroid Metabolites in Saliva Samples

Kataoka

Nakayama

2022

Molecules

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Accurate measurement of sulfated steroid metabolite concentrations can not only enable the elucidation of the mechanisms regulating steroid metabolism, but also lead to the diagnosis of various related diseases. The present study describes a simple and sensitive method for the simultaneous determination of four sulfated steroid metabolites in saliva, pregnenolone sulfate (PREGS), dehydroepiandrosterone sulfate (DHEAS), cortisol sulfate (CRTS), and 17β-estradiol-3-sulfate (E2S), by online coupling of in-tube solid-phase microextraction (IT-SPME) and stable isotope dilution liquid chromatography–tandem mass spectrometry (LC–MS/MS). These compounds were extracted and concentrated on Supel-Q PLOT capillary tubes by IT-SPME and separated and detected within 6 min by LC–MS/MS using an InertSustain swift C18 column and negative ion mode multiple reaction monitoring systems. These operations were fully automated by an online program. Calibration curves using their stable isotope-labeled internal standards showed good linearity in the range of 0.01–2 ng mL−1 for PREGS, DHEAS, and CRTS and of 0.05–10 ng mL−1 for E2S. The limits of detection (S/N = 3) of PREGS, DHEAS, CRTS, and E2S were 0.59, 0.30, 0.80, and 3.20 pg mL−1, respectively. Moreover, intraday and interday variations were lower than 11.1% (n = 5). The recoveries of these compounds from saliva samples were in the range of 86.6–112.9%. The developed method is highly sensitive and specific and can easily measure sulfated steroid metabolite concentrations in 50 μL saliva samples.

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Metabolomics prospect of obesity and metabolic syndrome; a systematic review

Payab

Tayanloo-Beik

Falahzadeh

et al. 2021

J Diabetes Metab Disord

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Metabolite profiling of sex developmental steroid conjugates reveals an association between decreased levels of steroid sulfates and adiposity in obese girls

Cited by 8 publications

References 27 publications

A systematic review of metabolomic studies of childhood obesity: State of the evidence for metabolic determinants and consequences

A systematic review of metabolomic studies of childhood obesity: State of the evidence for metabolic determinants and consequences

Online In-Tube Solid-Phase Microextraction Coupled with Liquid Chromatography–Tandem Mass Spectrometry for Automated Analysis of Four Sulfated Steroid Metabolites in Saliva Samples

Metabolomics prospect of obesity and metabolic syndrome; a systematic review

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