Metabolite profiling of Ricinus communis germination at different temperatures provides new insights into thermo-mediated requirements for successful seedling establishment

Ribeiro, Paulo R.; Willems, Leo A.J.; Mutimawurugo, Marie-Chantal; Fernandez, Luzimar Gonzaga; Castro, Renato Delmondez de; Ligterink, Wilco; Hilhorst, Henk W. M.

doi:10.1016/j.plantsci.2015.08.002

Cited by 20 publications

(21 citation statements)

References 44 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…radicle protrusion. This coincides with the thermo-sensitive window identified during seed germination in which high temperatures compromise seedling development (Ribeiro et al, 2015c).…”

Section: Mining For Similar Responses In Regulation Of Gene Expressiosupporting

confidence: 81%

“…We demonstrated that GABA levels were higher in 6 h imbibed seeds (6 hIS) samples imbibed at 20°C than at 35°C (Ribeiro et al, 2015c). Imbibing seeds possess very negative matrix potential because initially little or no water is left for uptake by the cells of the embryo.…”

Section: Additional Evidence To Support the Hypothesis That During Thmentioning

confidence: 87%

“…Therefore, using the concept that proximity means similarity, seeds that had imbibed at 20°C and 25°C seem to be closer related in terms of transcriptome, than seeds imbibed at 35°C. Principal component analysis applied to the overall variation in metabolite composition of R. communis seeds that germinated at 20°C, 25°C and 35°C indicated that most of the variation was attributed to differences in the developmental stage rather than to temperature (Ribeiro et al, 2015c). Therefore, transcriptome profiling provides additional and broader possibilities to identify the mechanisms underlying Ricinus communis thermotolerance during seed imbibition and germination.…”

Section: Pca Analysismentioning

confidence: 99%

“…The main reason to focus on the RP stage is because we have identified a temperature sensitive window during germination, i.e. before radicle protrusion, in which high temperatures compromise seedling development (Ribeiro et al, 2015c).…”

Section: Singular Enrichment Analysismentioning

confidence: 99%

“…The Brazilian National Program for Production and Use of Biodiesel has identified R. communis as the ideal oilseed crop to promote social development in the semi-arid region of Brazil because of its versatility as a productive (oil) crop in various environments (Cesar and Batalha, 2010). A thermo-sensitive window during R. communis seed germination in which high temperatures compromise seedling development has been previously identified (Ribeiro et al, 2015c). Nevertheless, there is a lack of information about the correlation between physiological events, such as germination and seedling establishment under high temperatures, and the associated transcriptome changes.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Ribeiro

Willems

Silva

et al. 2018

Industrial Crops and Products

Self Cite

View full text Add to dashboard Cite

A B S T R A C TRicinus communis seeds germinate to a high percentage and faster at 35°C than at lower temperatures, but with compromised seedling establishment and survival. However, seedlings are able to cope with high temperatures at later stages of seedling establishment if germination occurred at lower temperatures. The identification of this thermo-sensitive window during seed germination suggests that temperature disturb crucial mechanisms that support seedling establishment. We studied the molecular mechanisms that could explain this thermo-sensitive window with a genomics approach using microarray analysis to determine transcriptome changes during seed germination at 20, 25 and 35°C. Although temperature had a strong effect on the R. communis transcriptome, most of these differences occurred between 6 h of imbibition and the commencement of germination, i.e. radicle protrusion, which coincided with the identified thermo-sensitive window. We identified several responsive genes that might be involved in the thermotolerance of R. communis. Temperature had a major effect on genes involved in energy generating pathways, such as the Calvin-Benson-Bassham cycle, gluconeogenesis, and starch-and triacylglycerol degradation. Transcripts of ATP binding proteins, DNA binding proteins, RNA binding proteins, DNA-directed RNA polymerases, heat shock factor proteins, multiprotein-bridging factor proteins, and zinc finger proteins were also affected by temperature suggesting that transcriptional reprogramming mechanisms were disturbed. Among the downregulated transcripts, three were shared by all three stages: one oxidationrelated zinc finger 2, one F-box and wd40 domain protein, and one DNA binding protein/ MYB-like transcription factor. Among the upregulated transcripts, nine were shared by all three stages: one BET1P/SFT1P-like protein 14BB, one low-molecular-weight cysteine-rich protein LCR78, one WD-repeat protein, one GAST1 protein, one adenylate kinase 1/P-loop containing nucleoside triphosphate hydrolases superfamily protein, and four conserved hypothetical proteins. These genes constitute good candidate genes for further characterization of temperature-responsive molecular mechanisms in R. communis, which in turn will provide necessary tools for the exploitation of R. communis by small family farmers under the typical harsh conditions of semiarid regions worldwide.

show abstract

“…radicle protrusion. This coincides with the thermo-sensitive window identified during seed germination in which high temperatures compromise seedling development (Ribeiro et al, 2015c).…”

Section: Mining For Similar Responses In Regulation Of Gene Expressiosupporting

confidence: 81%

Section: Additional Evidence To Support the Hypothesis That During Thmentioning

confidence: 87%

Section: Pca Analysismentioning

confidence: 99%

Section: Singular Enrichment Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Ribeiro

Willems

Silva

et al. 2018

Industrial Crops and Products

Self Cite

View full text Add to dashboard Cite

show abstract

Genome‐wide characterization of the terpene synthase gene family in Ricinus communis and its transcriptional regulation under heat stress

et al. 2022

View full text Add to dashboard Cite

Terpene synthase (TPS) genes are responsible for producing highly specialized metabolites that assist in the plant's response to biotic and abiotic stresses, ecological signaling, and the regulation of homeostasis. Ricinus communis L. is an important oilseed crop that can grow in harsh environments and still display good yield. Thus, we aimed at characterizing the TPS gene family in R. communis and its transcriptional regulation under heat stress. For that, bioinformatics and transcriptomics were used to characterize the R. communis TPS (RcTPS) family and to pinpoint potential candidate genes for further genomics studies focused on stress-related agronomic problems. The R. communis genome possesses 46 TPS genes that were characterized by extensive bioinformatics, including phylogenetic analysis, subcellular localization, exon and intron distribution, and transcriptional regulatory regions. All 46 genes contained C-and N-terminal Pfam domains (PF01397 and PF03936) characteristics of the TPS enzymes. These genes also showed the conserved motifs DDXXD, DXDD, NSE/DTE, and RRX8W. The TPS sequences were aligned with several model plant species, and the presence of the canonical motif DDXXD amongst them indicates that TPS genes were highly conserved during plant evolution. Transcriptomics indicated that 37 RcTPS genes are readily responsive to high-temperature stress because they showed higher expression levels in seedlings growing at 35 ˚C compared with those growing at 20 and 25 ˚C. Altogether, TPS genes are potential candidates for genome editing techniques to develop abiotic stress-tolerant crops.

show abstract

Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed

Guo

Klinkesorn

Lorjaroenphon

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

Sprouts with higher levels of nutrients and lower content of antinutritional substances have been gained a growing interest in the influence on the human's health. The study of the influence of germination temperature and time on the metabolite profiles of sunflower seed was studied by a metabolomics approach based on gas chromatography–flame ionization detection (GC‐FID). Samples were extracted and fractionated covering a wide range of lipophilic and hydrophilic spectra. A total of 90 metabolites were identified by comparison with reference standards. Principal component analysis (PCA) revealed distinct dynamic changes in metabolites with the germinating time. Heatmap and agglomerative hierarchical clustering analysis revealed the differences and similarities among the samples. The germinating sunflower seeds clustered into three major groups. For instance, group I with a high content of sterols, monosaccharide, and amino acids, indicating the germination process, resulted in an increase in amino acids and monosaccharide. Group II had a high content of FAME and FFA. Relative targeted quantification of metabolites visually depicted by heatmap showed decreases in fatty acid methyl ester (FAME) and free fatty acid (FFA), and increases in amino acids, α‐tocopherol, sterols, and γ‐aminobutyric acid (GABA) during germination. Sunflower seeds germinated at 25°C were better for the accumulation of α‐tocopherol, stigmasterol, leucine, proline, methionine, glutamine, and GABA compared with those at 35°C. These results help to better understand how germination conditions change the nutritional quality of germinated sunflower seeds from a metabolite profile view, allowing for the rational screening and usage of germinated sunflower seeds in the food industry.

show abstract

Metabolite profiling of Ricinus communis germination at different temperatures provides new insights into thermo-mediated requirements for successful seedling establishment

Cited by 20 publications

References 44 publications

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Transcriptome profiling of Ricinus communis L. provides new insights underlying the mechanisms towards thermotolerance during seed imbibition and germination

Genome‐wide characterization of the terpene synthase gene family in Ricinus communis and its transcriptional regulation under heat stress

Effects of germinating temperature and time on metabolite profiles of sunflower (Helianthus annuus L.) seed

Contact Info

Product

Resources

About