Metabolite profiling of polyphenols in a Terminalia chebula Retzius ayurvedic decoction and evaluation of its chemopreventive activity

Pellati, Federica; Bruni, Renato; Righi, Davide; Grandini, Alessandro; Tognolini, Massimiliano; Prencipe, Francesco Pio; Poli, Ferruccio; Benvenuti, Stefania; Rio, Daniele Del; Rossi, Damiano

doi:10.1016/j.jep.2013.02.025

Cited by 51 publications

(31 citation statements)

References 40 publications

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“…The cytotoxic effect has been related to the presence of carvacrol, a phenolic compound. Presence of phenols in PAE has already been reported earlier in the preliminary phytochemical screening studies (Pellati et al, 2013;Rai et al, 2013). Yet another study has reported the IC 50 value of the crude extract from this plant to be 1382.8 µg/ml, in the same cell line (Hasibuan et al, 2013).…”

Section: Mtt Assay Of Phc and Pae With Mda-mb-231 Cell Linessupporting

confidence: 64%

A preliminary evaluation of anticancer and antioxidant potential of two traditional medicinal plants from Lamiaceae - Pogostemon heyneanus and Plectranthus amboinicus

Rai¹,

Pai²,

Kedilaya³

2016

J App Pharm Sci

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This study investigates the cytotoxic and antioxidant potential of crude leaf extracts of Pogostemon heyneanus (P. heyneanus) and Plectranthus amboinicus (P. amboinicus), medicinal plants from Lamiaceae family. Crude leaf extracts, with methanol, ethanol and chloroform were evaluated for cytotoxicity in two breast cancer cell lines of different grades, MCF-7 and MDA-MB-231, by MTT assay. The antioxidant activity of the extracts was evaluated by DPPH and H2O2 assays. The results of MTT assay revealed that chloroform extract of P. heyneanus and ethanol extract of P. amboinicus were effective against MCF-7 cells. The chloroform extract of P. heyneanus was more effective than P. amboinicus against MDA-MB-231. Effective antioxidant activity was shown by the ethanol extract of P. amboinicus which correlated positively with the total phenol content. The chloroform extract of P. heyneanus, was rich in steroids and saponins which correlated positively with the cytotoxic activity. In conclusion, chloroform soluble leaf extracts of P. heyneanus, which is rich in steroids and saponins shows effective cytotoxicity on both breast cancer lines studied and could be used for identification of a potential anticancer principle, while ethanol extract of P. amboinicus has better potential as an antioxidant.

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Section: Mtt Assay Of Phc and Pae With Mda-mb-231 Cell Linessupporting

confidence: 64%

A preliminary evaluation of anticancer and antioxidant potential of two traditional medicinal plants from Lamiaceae - Pogostemon heyneanus and Plectranthus amboinicus

Rai¹,

Pai²,

Kedilaya³

2016

J App Pharm Sci

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“…Currently, it is also reported that Chebulae Fructus possessed other various pleiotropic effects such as antioxidant, antidiabetic, renoprotective, hepatoprotective, immunomodulator and prokinetic effect (Chang & Lin 2012;Huang et al 2012;Sarkar et al 2012;Sasidharan et al 2012). Additionally, a number of chemical constituents have been isolated from the plant that include chebulin, ellagic acid, 2,4-chebulyl-D-glucopyranose, arjungenin, chebulinic acid, gallic acid, terflavin A, terchebin, luteolin and tannic acid (Pfundstein et al 2010;Pellati et al 2013;Singh & Kumar 2013). However, no systematic research has been carried out to examine the antidiarrhoeal activity of Chebulae Fructus extract so far.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the antidiarrhoeal properties of the aqueous extract and its soluble fractions of Chebulae Fructus (Terminalia chebula fruits)

Sheng

Yan

Zhang

et al. 2016

Pharmaceutical Biology

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Context Chebulae Fructus is used as an herbal remedy for diarrhoea in traditional Chinese medicine. However, there is no scientific evidence to support its antidiarrhoeal activity. Objective This study evaluates the antidiarrhoeal properties of Chebulae Fructus aqueous extract (CFAE) and determines the active fraction. Materials and methods The antidiarrhoeal effect of CFAE (200-800 mg/kg) was investigated by determining the wet dropping, intestinal transit in BALB/c mice and enteropooling in Wister rats. The protective effects of the CFAE on the intestinal and liver were tested by histopathological analyses. The antidiarrhoeal fraction was determined by castor oil-induced diarrhoea and its main constituents were identified by HPLC-ESI-MS.Results The extract at doses of 200, 400 and 800 mg/kg reduced the diarrhoea by 9.1, 40.0 and 58.2% and inhibited intestinal transit by 18.3, 24.1 and 35.7%, respectively. Additionally, the CFAE (200, 400 and 800 mg/kg) decreased the volume of enteropooling by 47.1, 58.8 and 64.7%, respectively. Mice treated with castor oil presented morphological alterations in the small intestine and the liver. However, the lesions of mice treated with CFAE were alleviated. Moreover, the ethyl acetate fraction was the active fraction of CFAE, the fraction (41.7, 83.4 and 166.8 mg/kg) reduced the diarrhoea by 9.1, 38.2 and 54.5%, respectively. The major components of the ethyl acetate fraction were tannins, including gallic acid, 3, 4, 6-tri-O-galloyl-b-D-Glc, corilagin and ellagic acid according to the HPLC-ESI-MS analysis. Conclusion The CFAE possessed antidiarrhoeal property and the ethyl acetate fraction was its main active fraction. ARTICLE HISTORY

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“…The decoction of T. chebula fruit was found to contain 3,4,6-tri-O-galloyl-d-glucose, chebulic acid, β-punicalagin, corilagin, α-punicalagin, chebulagic acid, gallic acid, 1,3,4,6-tri-Ogalloyl-β-d-glucose, chebulinic acid, 1,2,3,4,6-penta-O-galloyl-d-glucose, ellagic acid, 1,6-di-O-galloyl-d-glucose[38]. Flavonoids have also been reported to have significant antioxidant activity under in vivo as well as in vitro conditions[39].…”

mentioning

confidence: 99%

Antioxidant activity of polyphenolic extract ofTerminalia chebulaRetzius fruits

Saha

Verma

2016

Journal of Taibah University for Science

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The purpose of the present study was to investigate the in vitro antioxidant activities of polyphenolic extract of fruits of Terminalia chebula Retzius (Combretaceae). The polyphenolic extract of T. chebula fruits was evaluated for antioxidant activity by determining reducing power assay, total antioxidant capacity, DPPH radical (IC 50 14 µg/mL), nitric oxide radical (IC 50 30.51 µg/ml) and hydrogen peroxide scavenging assay (IC 50 265.53 µg/ml) under in vitro conditions. Moreover the phytochemical characterization of the extract was also measured by determining total phenolic, flavonoids, tannin and ascorbic acid contents. The characterization of the extract was also done by HPLC profiling with standard gallic acid. The present study demonstrated that extract have significant reducing capacity and scavenging of nitric oxide as well as hydrogen peroxide induced radicals. This activity of the extract may be due to the total polyphenolic contents present in it. The antioxidant activity of the extract was more significant than standard

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Metabolite profiling of polyphenols in a Terminalia chebula Retzius ayurvedic decoction and evaluation of its chemopreventive activity

Cited by 51 publications

References 40 publications

A preliminary evaluation of anticancer and antioxidant potential of two traditional medicinal plants from Lamiaceae - Pogostemon heyneanus and Plectranthus amboinicus

A preliminary evaluation of anticancer and antioxidant potential of two traditional medicinal plants from Lamiaceae - Pogostemon heyneanus and Plectranthus amboinicus

Assessment of the antidiarrhoeal properties of the aqueous extract and its soluble fractions of Chebulae Fructus (Terminalia chebula fruits)

Antioxidant activity of polyphenolic extract ofTerminalia chebulaRetzius fruits

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