Metabolite profiling of Panax notoginseng using UPLC–ESI-MS

Dan, Mo; Su, Mingming; Gao, Xianfu; Zhao, Tengteng; Zhao, Aihua; Xie, Guoxiang; Qiu, Yunping; Zhou, Mingmei; Zhong, Liu; Wang, Jia

doi:10.1016/j.phytochem.2008.04.015

Cited by 102 publications

(60 citation statements)

References 38 publications

(37 reference statements)

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“…So far, more than 80 ginsenosides have been isolated from over 10 Panax taxa, and most of these are derived from 4 types of aglycones: protopanaxadiol, protopanaxatriol, oleanolic acid, and ocotillol [9] . Biological activities, such as neuroprotective effects, antitumoral activity, and cardiac therapeutic effects have been documented for these ginsenosides [1,7,10] . The different sugar moieties in ginsenosides are assumed to provide specificity for the diverse therapeutic effects of variable ginsenosides.…”

Section: Introductionmentioning

confidence: 99%

Effect of sugar positions in ginsenosides and their inhibitory potency on Na+/K+-ATPase activity

Chen¹,

Chung²,

et al. 2008

Acta Pharmacol Sin

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Aim: To determine whether ginsenosides with various sugar attachments may act as active components responsible for the cardiac therapeutic effects of ginseng and sanqi (the roots of Panax ginseng and Panax notoginseng) via the same molecular mechanism triggered by cardiac glycosides, such as ouabain and digoxin. Methods: The structural similarity between ginsenosides and ouabain was analyzed. The inhibitory potency of ginsenosides and ouabain on Na+/K+-ATPase activity was examined and compared. Molecular modeling was exhibited for the docking of ginsenosides to Na+/K+-ATPase. Results: Ginsenosides with sugar moieties attached only to the C-3 position of the steroid-like structure, equivalent to the sugar position in cardiac glycosides, and possessed inhibitory potency on Na+/K+-ATPase activity. However, their inhibitory potency was significantly reduced or completely abolished when a monosaccharide was linked to the C-6 or C-20 position of the steroid-like structure; replacement of the monosaccharide with a disaccharide molecule at either of these positions caused the disappearance of the inhibitory potency. Molecular modeling and docking confirmed that the difference in Na+/K+-ATPase inhibitory potency among ginsenosides was due to the steric hindrance of sugar attachment at the C-6 and C-20 positions of the steroid-like structure. Conclusion: The cardiac therapeutic effects of ginseng and sanqi should be at least partly attributed to the effective inhibition of Na+/K+-ATPase by their metabolized ginsenosides with sugar moieties attached only to the C-3 position of the steroid-like structure

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Section: Introductionmentioning

confidence: 99%

Effect of sugar positions in ginsenosides and their inhibitory potency on Na+/K+-ATPase activity

Chen¹,

Chung²,

et al. 2008

Acta Pharmacol Sin

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“…Over the last few years, in many papers UHPLC fingerprints of herbal products were developed rather than the conventional HPLC approach [100][101][102][103][104][105][106][107][108][109][110][111][112]. Comparative studies between HPLC and UHPLC analyses on Hoodia, Cocoa, and Niu Huang Jie Du Pill samples reported a decreased analysis time by a factor up to eight without loss of information [102,111,112].…”

Section: Ultra-high Performance Liquid Chromatographymentioning

confidence: 99%

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

Tistaert

Dejaegher

Heyden

2011

Analytica Chimica Acta

320

275

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“…The UPLC separation is 5-10 times faster and has much better resolution than the normal analytical HPLC. 18 Combining UPLC with Q TOF-MS detector fulfills key requirements in terms of rapidity, sensitivity, selectivity, peak-assignment and quantification of the analytes at low concentrations in complex matrices. 19 Therefore, the present study was carried out to compare the potential in vitro produced culture of C. sinensis of Indian variety with natural drug in terms of the concentrations of kaempferol, isorhamnetin and cordycepin as determined by UPLC-QTOF-MS. …”

Section: Graphical Abstractmentioning

confidence: 99%

Quantification of Flavonoids and Nucleoside by UPLC-MS in Indian Cordyceps sinensis and its invitro Cultures

Khan¹,

Khan²,

Manickam³

et al. 2015

IJPER

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Background: Cordyceps sinensis, a comestible mushroom growing in Himalayan regions, is extensively familar in traditional system of medicine. The paper presents the results of quantification of some of the flavonoids and nucleosides (kaempferol, isorhamnetin and cordycepin) in the Indian variety of Cordyceps sinensis. Since, the plant is known for its high therapeutic potential and not easily available, hence it was also tissue cultured in the laboratory as an alternative source of medicine. Methods: Ultra Performance Liquid Chromatography (UPLC) along with Q-TOF-MS detector was used for the characterization and quantification of active components in the aqueous extracts of both the samples of Cordyceps sinensis (natural as well as in vitro cultured). Results: Results indicate that both the extracts have almost similar concentration of the kaempferol, isorhamnetin and cordycepin which supports use of in vitro biomass as an alternative source of medicine.

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Metabolite profiling of Panax notoginseng using UPLC–ESI-MS

Cited by 102 publications

References 38 publications

Effect of sugar positions in ginsenosides and their inhibitory potency on Na+/K+-ATPase activity

Effect of sugar positions in ginsenosides and their inhibitory potency on Na+/K+-ATPase activity

Chromatographic separation techniques and data handling methods for herbal fingerprints: A review

Quantification of Flavonoids and Nucleoside by UPLC-MS in Indian Cordyceps sinensis and its invitro Cultures

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