Metabolite profiling of mycorrhizal roots of Medicago truncatula

Schliemann, Willibald; Ammer, Christian; Strack, Dieter

doi:10.1016/j.phytochem.2007.06.032

Cited by 236 publications

(202 citation statements)

References 201 publications

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“…Veronica chamaedrys (Vc, Plantaginaceae) was included to allow betweengenus comparisons within the same plant family. Medicago truncatula (Mt, Fabaceae), for which the association with root symbionts is well characterized 8,25 , represented another dicot family, whereas Poa annua (Pa, Poaceae) was included as representative of the monocots. The chosen species are herbaceous with comparable statures and share largely similar habitats.…”

Section: Resultsmentioning

confidence: 99%

“…Third, arbuscular mycorrhizas are of considerable agricultural importance, as AMF enhance nutritional values and yields of crops, modulate plant pest and stress resistance, and influence global C and P cycling, ecosystem services, and primary productivity [15][16][17] . In contrast to the roots, whose metabolism under AM symbiosis is well characterized 8 , systemic effects of AMF, whose morphological structures are restricted to the roots, on shoot metabolic phenotypes are sparse and were mainly studied for certain targets or metabolomes of single species yet 6,[18][19][20][21][22][23] .…”

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confidence: 99%

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High specificity in plant leaf metabolic responses to arbuscular mycorrhiza

et al. 2014

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The chemical composition of plants (phytometabolome) is dynamic and modified by environmental factors. Understanding its modulation allows to improve crop quality and decode mechanisms underlying plant-pest interactions. Many studies that investigate metabolic responses to the environment focus on single model species and/or few target metabolites. However, comparative studies using environmental metabolomics are needed to evaluate commonalities of chemical responses to certain challenges. We assessed the specificity of foliar metabolic responses of five plant species to the widespread, ancient symbiosis with a generalist arbuscular mycorrhizal fungus. Here we show that plant species share a large 'core metabolome' but nevertheless the phytometabolomes are modulated highly species/taxon-specifically. Such a low conservation of responses across species highlights the importance to consider plant metabolic prerequisites and the long time of specific plant-fungus coevolution. Thus, the transferability of findings regarding phytometabolome modulation by an identical AM symbiont is severely limited even between closely related species.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

High specificity in plant leaf metabolic responses to arbuscular mycorrhiza

et al. 2014

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“…To get data for their structures, the Apo1-and Apo2-containing fractions were subjected to liquid chromatography electrospray ionization mass spectrometry (LC-ESI/MS) analyses. Unfortunately, Apo1 coeluted with a compound showing a MS fragmentation pattern identical to that of the known root saponin bayogenin dihexoside (Huhman and Sumner, 2002;Huhman et al, 2005;Schliemann et al, 2008a), precluding identification. However, it was possible to identify Apo2.…”

Section: Mtccd1-silenced Rootsmentioning

confidence: 99%

RNA Interference-Mediated Repression of MtCCD1 in Mycorrhizal Roots of Medicago truncatula Causes Accumulation of C27 Apocarotenoids, Shedding Light on the Functional Role of CCD1

et al. 2008

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Tailoring carotenoids by plant carotenoid cleavage dioxygenases (CCDs) generates various bioactive apocarotenoids. Recombinant CCD1 has been shown to catalyze symmetrical cleavage of C 40 carotenoid substrates at 9,10 and 9#,10# positions. The actual substrate(s) of the enzyme in planta, however, is still unknown. In this study, we have carried out RNA interference (RNAi)-mediated repression of a Medicago truncatula CCD1 gene in hairy roots colonized by the arbuscular mycorrhizal (AM) fungus Glomus intraradices. As a consequence, the normal AM-mediated accumulation of apocarotenoids (C 13 cyclohexenone and C 14 mycorradicin derivatives) was differentially modified. Mycorradicin derivatives were strongly reduced to 3% to 6% of the controls, while the cyclohexenone derivatives were only reduced to 30% to 47%. Concomitantly, a yellow-orange color appeared in RNAi roots. Based on ultraviolet light spectra and mass spectrometry analyses, the new compounds are C 27 apocarotenoic acid derivatives. These metabolic alterations did not lead to major changes in molecular markers of the AM symbiosis, although a moderate shift to more degenerating arbuscules was observed in RNAi roots. The unexpected outcome of the RNAi approach suggests C 27 apocarotenoids as the major substrates of CCD1 in mycorrhizal root cells. Moreover, literature data implicate C 27 apocarotenoid cleavage as the general functional role of CCD1 in planta. A revised scheme of plant carotenoid cleavage in two consecutive steps is proposed, in which CCD1 catalyzes only the second step in the cytosol (C 27 / C 14 + C 13 ), while the first step (C 40 / C 27 + C 13 ) may be catalyzed by CCD7 and/or CCD4 inside plastids.

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“…The field of metabolomics is now routine for the identification of primary and secondary metabolites in plant taking advantage of mass spectrometry (MS) [3][4][5][6] and for reaching a metabolic fingerprinting in plant tissues by both nuclear magnetic resonance (NMR) spectroscopy [7] and MS [8]. Fingerprinting is often used as a complementary tool to differentiate genetically modified species from pools of modified species [9], or to follow changes in the entire metabolism brought about by a specific treatment [10]. The latter application was already proved to efficiently differentiate mycorrhiza-treated plants from control [10].…”

Section: Introductionmentioning

confidence: 99%

“…Fingerprinting is often used as a complementary tool to differentiate genetically modified species from pools of modified species [9], or to follow changes in the entire metabolism brought about by a specific treatment [10]. The latter application was already proved to efficiently differentiate mycorrhiza-treated plants from control [10]. However, most studies have been focused on the mechanisms by which microbial treatments affect plants preferentially from a genomic and transcriptomic standpoint [11,12], thereby indicating that there is still ample margin to develop the metabolomic approach.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Nebbioso¹,

Martino

Eltlbany

et al. 2016

Chem. Biol. Technol. Agric.

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Background: In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains of microorganisms (B1-B4) capable of positively affecting plant growth. The methanolic extracts from treated roots were analysed by reverse phase ultra-high-performance liquid chromatography hyphenated with ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites with that of control plants (B0). Results:We found, in both treated and control plants, several primary metabolites, such as fatty acids and coumaric acid, and other compounds associated with secondary metabolism pathways such as that of cyclopentaneoctanoic acid (CPOA) or hydroxyoctadecadienoic acid (HODE), and additional molecules which were not characterizable with the available data. A semiquantitative assessment of all metabolites became the basis for further processing the metabolic results by principal component analysis, which highlighted significant differences in the PC1 and PC2 components. The PC1 was particularly affected by the presence of arachidonic acid, myristic acid, and two unidentified metabolites. It effectively differentiated control plants from all bioeffectors treatments, and, in particular, the B4 treatment from the rest (B1-B3). The PC2 was mainly affected by palmitic acid, heptadecanoic acid, two CPOAs, one HODE and two unidentified metabolites. These metabolites successfully differentiated the B0 control from all the bioeffectors treatments, and, especially, showed a difference between B1 and B2. Conclusions:Our findings suggest that changes in secondary pathways of lipid metabolism may underlie the biostimulation exerted by the four microbial bioeffectors of this study, and that LC-MS coupled by multivariate analysis can easily fingerprint the metabolic alterations induced by bioeffectors in tomato roots.

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Metabolite profiling of mycorrhizal roots of Medicago truncatula

Cited by 236 publications

References 201 publications

High specificity in plant leaf metabolic responses to arbuscular mycorrhiza

High specificity in plant leaf metabolic responses to arbuscular mycorrhiza

RNA Interference-Mediated Repression of MtCCD1 in Mycorrhizal Roots of Medicago truncatula Causes Accumulation of C27 Apocarotenoids, Shedding Light on the Functional Role of CCD1

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

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